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@@ -9,20 +9,7 @@ R2 Implantation System
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The **R2 system** is an open-source implantation system for silicon probes. R2 stands for Recover and Reuse those silicon probes, on average for 3 chronic experiments. This defining feature is enabled by sacrificing the lower end of the implant during explantation. As a result, the expensive payload is safely recovered while the inexpensive, replaceable part is disposed of.
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.. important::
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When using the R2 System, please cite the following paper:
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Mihály Vöröslakos, Peter C Petersen, Balázs Vöröslakos, György Buzsáki (2021)
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Metal microdrive and head cap system for silicon probe recovery in freely moving rodent eLife 10:e65859.
Please also mention 3Dneuro as the manufacturer of the R2 system in your methods section.
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The **R2 system** is an open-source implantation system for silicon probes. R2 stands for Recover and Reuse those silicon probes, on average for 3 chronic experiments. This defining feature is enabled by sacrificing the lower end of the implant during explantation. As a result, the expensive payload is safely recovered while the inexpensive, replaceable part can be disposed of.
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Components
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* Metal parts ensure performance and durability for key steps such as probe recovery
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* Probe recovery rate 70-80% initially, 100% with experience; Probe reuse 3+ times, until the impedance degrades
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* Ability to move the probe post-implantation with microdrive \- 7mm travel distance
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* Lightweight: Microdrive \= 0.47g; Mouse cap \=1.57g; Rat cap \=8.3g
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* Lightweight: Microdrive \= 0.47g; Mouse cap \=2.2g; Rat cap \=11g
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* Components form a fully integrated system but can be used independently
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* Extensive documentation \- 4 published protocols
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* Extensive documentation, with 4 published protocols
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R2drive stands out by the abundance of protocols available in peer-reviewed journals, which reflect its continuous development. In addition to the original methods paper by `Vöröslakos et al. <https://doi.org/10.7554/eLife.65859>`__, the `Buzsaki lab <https://buzsakilab.com/wp/>`__ at NYU also published further protocols that describe the use of a `base plate system <https://doi.org/10.21769/BioProtoc.4137>`__ and of `μLED probes for optogenetic perturbation <https://doi.org/10.1016/j.xpro.2023.102570>`__. 3Dneuro participated in the design of `a novel head-fixation and headgear system <https://doi.org/10.3791/66867>`__, and is continuously improving the design and manufacturing process based on user feedback. Several research papers using the R2 system have already been published (e.g. `Huszár et al. 2022 <https://www.nature.com/articles/s41593-022-01138-x>`__, `Watkins de Jong et al. 2023 <https://www.sciencedirect.com/science/article/abs/pii/S0960982223003202>`__, `Soula et al. 2023 <https://www.pnas.org/doi/10.1073/pnas.2302676120>`__, `Ye et al 2024 <https://www.biorxiv.org/content/10.1101/2023.08.23.554527v3>`__).
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.. important::
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When using the R2 System, please cite the following paper:
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Mihály Vöröslakos, Peter C Petersen, Balázs Vöröslakos, György Buzsáki (2021)
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Metal microdrive and head cap system for silicon probe recovery in freely moving rodent eLife 10:e65859.
R2drive stands out by the abundance of protocols available in peer-reviewed journals, which reflect its continuous development. In addition to the original methods paper by `Vöröslakos et al. <https://doi.org/10.7554/eLife.65859>`__ (the reference about the R2 system, please cite if you use it), the `Buzsaki lab <https://buzsakilab.com/wp/>`__ at NYU also published further protocols that describe the use of a `base plate system <https://doi.org/10.21769/BioProtoc.4137>`__ and of `μLED probes for optogenetic perturbation <https://doi.org/10.1016/j.xpro.2023.102570>`__. 3Dneuro participated in the design of `a novel head-fixation and headgear system <https://doi.org/10.3791/66867>`__, and is continuously improving the design and manufacturing process based on user feedback. Several research papers using the R2 system have also been published (e.g. `Huszár et al. 2022 <https://www.nature.com/articles/s41593-022-01138-x>`__, `Watkins de Jong et al. 2023 <https://www.sciencedirect.com/science/article/abs/pii/S0960982223003202>`__, `Soula et al. 2023 <https://www.pnas.org/doi/10.1073/pnas.2302676120>`__, `Ye et al 2024 <https://www.biorxiv.org/content/10.1101/2023.08.23.554527v3>`__)
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Please also consider mentioning 3Dneuro in your methods section as the supplier of the R2 system.
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