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- segment: a centerline piece between two junctions or a junction and an endpoint
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- filament: a whole connected vessel path or connected component of the skeleton
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- curvature: mean angular change along the skeleton trace per step
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- tortuosity: ratio of actual path length to end-to-end straight-line distance (segment_length / e2e_distance)
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- straightness: inverse of tortuosity (e2e_distance / segment_length)
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- lacunarity: measures the gappiness or heterogeneity of a spatial pattern. Computed by sliding a box of varying sizes across the image and measuring the variance in pixel occupancy. High lacunarity = large, uneven gaps (clumpy, patchy coverage). Low lacunarity = small, evenly distributed gaps (homogeneous, uniform coverage). Two patterns can have the same area fraction (i.e. same vessel density) but very different lacunarity depending on how the gaps are distributed.
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- hgu: hyphal growth unit = total vessel length / endpoint count (μm). Represents the average uninterrupted fibre length before hitting an endpoint; higher HGU means longer fibre stretches with fewer breaks
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- box_counting_fractal_dimension: fractal dimension of the fibre mask computed by counting how many boxes of varying sizes are needed to cover the pattern. Ranges ~1–2 for 2D images. Values near 2 mean fibres nearly fill the plane; values near 1 mean sparse, line-like coverage
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- high_density_matrix: fraction of image pixels (0–1) classified as especially dense ECM. An extra intensity threshold is applied on top of the fibre mask to isolate the brightest/most tightly packed fibre regions from the general mask.
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- alignment_coherency: percentage (0–100%) measuring how uniformly fibres point in the same direction. 0% = isotropic (fibres point in all directions equally with no preferred orientation, like scattered toothpicks). 100% = all fibres are perfectly parallel (like combed hair). Computed via OrientationJ dominant direction analysis.
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- gap_area: area of an inscribed circle (px) fitted into the empty spaces between fibres. Captures the size of holes in the fibre network. Reported as summary statistics (mean, sd, 5th/50th/95th percentiles) and as a per-gap array suitable for histograms or distribution analysis

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