|
| 1 | +import argparse |
| 2 | +import json |
| 3 | +from typing import ( |
| 4 | + Any, |
| 5 | + Callable, |
| 6 | +) |
| 7 | + |
| 8 | +import giatools |
| 9 | +import numpy as np |
| 10 | +import scipy.ndimage as ndi |
| 11 | +from skimage.morphology import disk |
| 12 | + |
| 13 | + |
| 14 | +def image_astype(img: giatools.Image, dtype: np.dtype) -> giatools.Image: |
| 15 | + return giatools.Image( |
| 16 | + data=img.data.astype(dtype), |
| 17 | + axes=img.axes, |
| 18 | + original_axes=img.original_axes, |
| 19 | + metadata=img.metadata, |
| 20 | + ) |
| 21 | + |
| 22 | + |
| 23 | +filters = { |
| 24 | + 'gaussian': lambda img, sigma, order=0, axis=None: ( |
| 25 | + apply_2d_filter( |
| 26 | + ndi.gaussian_filter, |
| 27 | + img if order == 0 else image_astype(img, float), |
| 28 | + sigma=sigma, |
| 29 | + order=order, |
| 30 | + axes=axis, |
| 31 | + ) |
| 32 | + ), |
| 33 | + 'uniform': lambda img, size: ( |
| 34 | + apply_2d_filter(ndi.uniform_filter, img, size=size) |
| 35 | + ), |
| 36 | + 'median': lambda img, radius: ( |
| 37 | + apply_2d_filter(ndi.median_filter, img, footprint=disk(radius)) |
| 38 | + ), |
| 39 | + 'prewitt': lambda img, axis: ( |
| 40 | + apply_2d_filter(ndi.prewitt, img, axis=axis) |
| 41 | + ), |
| 42 | + 'sobel': lambda img, axis: ( |
| 43 | + apply_2d_filter(ndi.sobel, img, axis=axis) |
| 44 | + ), |
| 45 | +} |
| 46 | + |
| 47 | + |
| 48 | +def apply_2d_filter( |
| 49 | + filter_impl: Callable[[np.ndarray, Any, ...], np.ndarray], |
| 50 | + img: giatools.Image, |
| 51 | + **kwargs: Any, |
| 52 | +) -> giatools.Image: |
| 53 | + """ |
| 54 | + Apply the 2-D filter to the 2-D/3-D, potentially multi-frame and multi-channel image. |
| 55 | + """ |
| 56 | + result_data = None |
| 57 | + for qtzc in np.ndindex( |
| 58 | + img.data.shape[ 0], # Q axis |
| 59 | + img.data.shape[ 1], # T axis |
| 60 | + img.data.shape[ 2], # Z axis |
| 61 | + img.data.shape[-1], # C axis |
| 62 | + ): |
| 63 | + sl = np.s_[*qtzc[:3], ..., qtzc[3]] # noqa: E999 |
| 64 | + arr = img.data[sl] |
| 65 | + assert arr.ndim == 2 # sanity check, should always be True |
| 66 | + |
| 67 | + # Perform 2-D filtering |
| 68 | + res = filter_impl(arr, **kwargs) |
| 69 | + if result_data is None: |
| 70 | + result_data = np.empty(img.data.shape, res.dtype) |
| 71 | + result_data[sl] = res |
| 72 | + |
| 73 | + # Return results |
| 74 | + return giatools.Image(result_data, img.axes) |
| 75 | + |
| 76 | + |
| 77 | +def apply_filter( |
| 78 | + input_filepath: str, |
| 79 | + output_filepath: str, |
| 80 | + filter_type: str, |
| 81 | + **kwargs: Any, |
| 82 | +): |
| 83 | + # Read the input image |
| 84 | + img = giatools.Image.read(input_filepath) |
| 85 | + |
| 86 | + # Perform filtering |
| 87 | + filter_impl = filters[filter_type] |
| 88 | + res = filter_impl(img, **kwargs).normalize_axes_like(img.original_axes) |
| 89 | + |
| 90 | + # Adopt metadata and write the result |
| 91 | + res.metadata = img.metadata |
| 92 | + res.write(output_filepath, backend='tifffile') |
| 93 | + |
| 94 | + |
| 95 | +if __name__ == "__main__": |
| 96 | + parser = argparse.ArgumentParser() |
| 97 | + parser.add_argument('input', type=str, help='Input image filepath') |
| 98 | + parser.add_argument('output', type=str, help='Output image filepath (TIFF)') |
| 99 | + parser.add_argument('params', type=str) |
| 100 | + args = parser.parse_args() |
| 101 | + |
| 102 | + # Read the config file |
| 103 | + with open(args.params) as cfgf: |
| 104 | + cfg = json.load(cfgf) |
| 105 | + |
| 106 | + apply_filter( |
| 107 | + args.input, |
| 108 | + args.output, |
| 109 | + **cfg, |
| 110 | + ) |
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