Question regarding the self-targeting

[Dfvandenberg]

Hi,

I am wanting to use your tool to explore self-targeting, however, I did a known control: CP009676.1 (Staphylococcus schleiferi strain 5909-02) and found that all spacers are self-targeting, while originally there were only 3 found and crisprminer2 finds 6 self-targeting ones. I was wondering if there is a possibility to make the self-targeting more strict in order to give the same result as crisprminer2.

Also, the -st virus function gives some strange errors:
###################################################################################
########## CRISPR: Master Script #########
###################################################################################

Traceback (most recent call last):
File "../CRISPRloci-1.0.0/CRISPRloci_standalone.py", line 1513, in
crispr_virus()
File "../CRISPRloci-1.0.0/CRISPRloci_standalone.py", line 257, in crispr_virus
dirname_virus = curPath + '/' + dirname_virus
TypeError: unsupported operand type(s) for +: 'PosixPath' and 'str'

And when using the full path, it is able to build the protein database, but :
Traceback (most recent call last):
File "CRISPRVirusSpacers.py", line 163, in
Map_Sortname_FullnameDB = read_map("Map_Sortname_FullnameDB.fa")
File "CRISPRVirusSpacers.py", line 71, in read_map
Map = open(cd_path + "/NewDB/" + file_name, "r")

With kind regards,
Daan