improvements to the Data module

[aryarm]

input files

would be nice if we could support the following inputs

• Path objects representing paths to the files
  • and files ending in gz
• sys.stdout and sys.stdin
  • this might be harder for the Genotypes class b/c I think cyvcf2 only accepts strings to paths (see Read VCF From StringIO or Buffer? brentp/cyvcf2#47)
• TextIO objects
  • This definitely won't be possible for the Genotypes class but we could do it for the Phenotypes and Covariates classes?

one strategy would be to create a function in the Data abstract class that could detect each of these cases and handle them appropriately?

• we should also ensure that most of the classes can work appropriately on streams of data
  • and rewrite Genotypes.read to allow it to read data line by line

informative warnings

• would also be nice if we could warn users when the regions or samples that they provided encompass zero variants
  • and tell them to check that the chroms prefix matches up or attempt to fix it ourselves
• for all warnings and errors, use the Logger module instead of raising assertions?

additional classes

• for covariates (as a table of samples x covariates)

filtering of variants

• by whether they're multi-allelic
• automatically by the subset of samples contained in the intersection of the genotype and phenotype files
  • note that this might be something we should only do within the code that utilizes the data module (for ex: happler)
• by MAF

subclasses for different kinds of genotyping data

or just some way to type-hint the specific kind that you need

• phased vs no restriction on phasing
• biallelic vs no restriction on allele number
  • filterable for above a certain MAF (only applies to biallelic)
• contains TRs (potentially handled by trtools - see support for a TR-based GenotypesPLINK class #73)

new functions

• iterate() - a generator function that iterates over each line bit by bit and yields named tuples where each entry is a property of the module but having values just for a single row

[aryarm]

For the Genotypes class

• create some way to quickly obtain the index of a variant based on its ID
  • add a dictionary
• remove aaf from Genotypes.variants
  • it was never really useful to begin with, anyway - just a bad idea from the start
  • we can make it into a method, instead
• also, remove the Genotypes.to_MAC() method
• add subset() function to the Genotypes class
  • by default, return a new Genotypes instance unless the inplace parameter is set to True
• numpy-based subsetting and indexing
  • implement __getitem__()
  • implement __setitem__()
  • implement __delitem__()?
• a method to generate fake Genotypes
• Compare genotypes read when _prephased=True and _prephased=False in the GenotypesPLINK class. Figure out why they're different
  • update: I made an attempt at doing this in test: hidden phasing property in GenotypesPLINK class #153 but couldn't reproduce the issue
• create a QC method for running all of the QC steps?
• reduce memory usage by explicitly freeing memory after loading every chunk of a PGEN file
  Right after this line of code, add the following lines:

  del data
  gc.collect()
  

  (source and source)
  Update: current progress on this

For the Phenotypes class

• allow for storing multiple phenotypes in the Phenotypes object
• support PLINK2-style .pheno files
  • writing
  • reading
• make the Covariates class into a subclass of the Phenotypes object
• add a method to generate fake Phenotypes
• add subset() function for choosing a subset of samples
• change the type of the samples argument for the Phenotypes class to a set (refactor: data.Phenotypes samples parameter to be of type set #152)

For the Haplotype and Haplotypes classes

• use Genotypes.subset() within the transform() methods
  • remove the samples arguments from each of the transform() methods
• do not require an empty GenotypesRefAlt class as input to Haplotypes.transform()
• add subset() function for choosing a subset of haplotypes after loading them
• require that the haplotypes parameter of the Haplotypes.read() method be a set instead of a list

For the Haplotype and Variant classes

• make it easier to extend the classes, so that extras don't have to be declared ahead of time? pros: it would make it easier to read files with multiple different sets of extra fields; but cons: it puts the burden of handling all of that on us, which could potentially be difficult to take on in the future

For the Haplotype class

• create a method that will update the start and end coordinates according to the stored variants