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README.md

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@@ -21,7 +21,7 @@ Clone the Repository: Clone the new repository to your local machine, choosing t
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### Step 2: Configure workflow
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A) #### Have input RawCountFile_rsemgenes.txt available in test folder.
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1) #### Have input RawCountFile_rsemgenes.txt available in test folder.
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-Example of input RawCountFile_rsemgenes.txt
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| gene_id | Sample1 | Sample2 | Sample3 | Sample4 | Sample5 | Sample6 |
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|----------------------------|---------|---------|---------|---------|---------|---------|
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| ENSG00000005955.13_ACTN2 | 500 | 530 | 460 | 550 | 540 | 570 |
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| ENSG00000006062.15_RPS6 | 600 | 650 | 580 | 700 | 680 | 720 |
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B) #### Modify the contrasts.txt file in config folder.
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2) #### Modify the contrasts.txt file in config folder.
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Add sample condition contrast pairs.
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Example:
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| parent | control |
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|--------|---------|
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C) #### Modify the sampleinfo.txt file in config folder.
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3) #### Modify the sampleinfo.txt file in config folder.
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Add sample details keeping the same format such as:
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| samplename | condition | batch |
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|------------|-----------|-------|
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| Sample5 | control | b2 |
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| Sample6 | control | b2 |
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D) #### In config.yaml:
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4) #### In config.yaml:
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Give full path to raw_counts data
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Give full path to results directory
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Select the kegg_organism. For example: "mmu" for mouse
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E) #### In Snakefile:
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5) #### In Snakefile:
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Give full path to configfile
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Give full path to Snakemake rule files
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F) #### In the common.smk:
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6) #### In the common.smk:
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Give full path to configfile
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G) #### Ensure full paths to the Rscripts in snakemake (.smk) files under 'shell' or "params' for the following:
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7) #### Ensure full paths to the Rscripts in snakemake (.smk) files under 'shell' or "params' for the following:
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deseq2.smk
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pathway.smk
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limma_voom.smk
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H) #### Ensure full path to R libraries as .libPaths in pathway.R
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8) #### Ensure full path to R libraries as .libPaths in pathway.R
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### Step 3: Load the snakemake version 8 or above
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