@@ -21,7 +21,7 @@ Clone the Repository: Clone the new repository to your local machine, choosing t
2121
2222### Step 2: Configure workflow
2323
24- A ) #### Have input RawCountFile_rsemgenes.txt available in test folder.
24+ 1 ) #### Have input RawCountFile_rsemgenes.txt available in test folder.
2525-Example of input RawCountFile_rsemgenes.txt
2626| gene_id | Sample1 | Sample2 | Sample3 | Sample4 | Sample5 | Sample6 |
2727| ----------------------------| ---------| ---------| ---------| ---------| ---------| ---------|
@@ -34,13 +34,13 @@ A) #### Have input RawCountFile_rsemgenes.txt available in test folder.
3434| ENSG00000005955.13_ACTN2 | 500 | 530 | 460 | 550 | 540 | 570 |
3535| ENSG00000006062.15_RPS6 | 600 | 650 | 580 | 700 | 680 | 720 |
3636
37- B ) #### Modify the contrasts.txt file in config folder.
37+ 2 ) #### Modify the contrasts.txt file in config folder.
3838Add sample condition contrast pairs.
3939Example:
4040| parent | control |
4141| --------| ---------|
4242
43- C ) #### Modify the sampleinfo.txt file in config folder.
43+ 3 ) #### Modify the sampleinfo.txt file in config folder.
4444Add sample details keeping the same format such as:
4545| samplename | condition | batch |
4646| ------------| -----------| -------|
@@ -51,7 +51,7 @@ Add sample details keeping the same format such as:
5151| Sample5 | control | b2 |
5252| Sample6 | control | b2 |
5353
54- D ) #### In config.yaml:
54+ 4 ) #### In config.yaml:
5555Give full path to raw_counts data
5656
5757Give full path to results directory
@@ -64,15 +64,15 @@ Select the organism_db accordingly. For example: "org.Mm.eg.db" for mouse
6464
6565Select the kegg_organism. For example: "mmu" for mouse
6666
67- E ) #### In Snakefile:
67+ 5 ) #### In Snakefile:
6868Give full path to configfile
6969
7070Give full path to Snakemake rule files
7171
72- F ) #### In the common.smk:
72+ 6 ) #### In the common.smk:
7373Give full path to configfile
7474
75- G ) #### Ensure full paths to the Rscripts in snakemake (.smk) files under 'shell' or "params' for the following:
75+ 7 ) #### Ensure full paths to the Rscripts in snakemake (.smk) files under 'shell' or "params' for the following:
7676deseq2.smk
7777
7878pathway.smk
@@ -85,7 +85,7 @@ rmarkdown.smk
8585
8686limma_voom.smk
8787
88- H ) #### Ensure full path to R libraries as .libPaths in pathway.R
88+ 8 ) #### Ensure full path to R libraries as .libPaths in pathway.R
8989
9090### Step 3: Load the snakemake version 8 or above
9191
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