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Copy file name to clipboardExpand all lines: docs/balsamic_methods.rst
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Target Genome Analysis
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~~~~~~~~~~~~~~~~~~~~~~
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BALSAMIC :superscript:`1` (**version** = 18.0.0) was used to analyze the data from raw FASTQ files.
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BALSAMIC :superscript:`1` (**version** = 18.0.1) was used to analyze the data from raw FASTQ files.
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We first quality controlled FASTQ files using FastQC v0.11.9 :superscript:`2`.
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Adapter sequences are trimmed using fastp v0.23.2 :superscript:`3` and then UMI sequences are extracted using the UMI extract tool from sentieon-tools (version 202308.03) :superscript:`15` and finally low-quality bases were trimmed using fastp v0.23.2 :superscript:`3`.
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Trimmed reads were mapped to the reference genome hg19 using sentieon-tools :superscript:`15`.
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Whole Genome Analysis
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~~~~~~~~~~~~~~~~~~~~~
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BALSAMIC :superscript:`1` (**version** = 18.0.0) was used to analyze the data from raw FASTQ files.
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BALSAMIC :superscript:`1` (**version** = 18.0.1) was used to analyze the data from raw FASTQ files.
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We first quality controlled FASTQ files using FastQC v0.11.9 :superscript:`2`.
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Adapter sequences and low-quality bases were trimmed using fastp v0.23.2 :superscript:`3`.
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Trimmed reads were mapped to the reference genome hg19 using sentieon-tools 202308.03 :superscript:`15`.
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UMI workflow
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~~~~~~~~~~~~~~~~~~~~~
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BALSAMIC :superscript:`1` (**version** = 18.0.0) was used to analyze the data from raw FASTQ files.
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BALSAMIC :superscript:`1` (**version** = 18.0.1) was used to analyze the data from raw FASTQ files.
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We first quality controlled FASTQ files using FastQC v0.11.9 :superscript:`2`.
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Adapter sequences were trimmed using fastp v0.23.2 :superscript:`3`.
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UMI tag extraction and alignment and consensus-calling of UMI groups were performed using Sentieon tools 202308.03 :superscript:`15`.
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