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Two images broken (Error instantiating pixel buffer) #1

@dominikl

Description

@dominikl

https://idr.openmicroscopy.org/webclient/img_detail/13425387/?dataset=15003
( /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack.ome.tif)

https://idr.openmicroscopy.org/webclient/img_detail/13425427/?dataset=15005
(/uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-TPR/02_638i100_640i150_405i50_x20_1_MMStack2.ome.tif)

The problem: This files reference other tifs which are not there (have been renamed). I don't know why this error shows up now, and didn't do so when the submission was imported initially. However, I tried to fix by creating symlinks with the expected file name pointing to the renamed tifs:

cd /nfs/ebi/bioimage/drop/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2
ln -s 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_10.ome.tif 01_638i100_640i150_405i50_x20_1_MMStack_10.ome.tif
ln -s 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_11.ome.tif 01_638i100_640i150_405i50_x20_1_MMStack_11.ome.tif
ln -s 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_12.ome.tif 01_638i100_640i150_405i50_x20_1_MMStack_12.ome.tif
ln -s 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_13.ome.tif 01_638i100_640i150_405i50_x20_1_MMStack_13.ome.tif
ln -s 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_1.ome.tif 01_638i100_640i150_405i50_x20_1_MMStack_1.ome.tif
ln -s 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_2.ome.tif 01_638i100_640i150_405i50_x20_1_MMStack_2.ome.tif
ln -s 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_3.ome.tif 01_638i100_640i150_405i50_x20_1_MMStack_3.ome.tif
ln -s 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_4.ome.tif 01_638i100_640i150_405i50_x20_1_MMStack_4.ome.tif
ln -s 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_5.ome.tif 01_638i100_640i150_405i50_x20_1_MMStack_5.ome.tif
ln -s 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_6.ome.tif 01_638i100_640i150_405i50_x20_1_MMStack_6.ome.tif
ln -s 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_7.ome.tif 01_638i100_640i150_405i50_x20_1_MMStack_7.ome.tif
ln -s 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_8.ome.tif 01_638i100_640i150_405i50_x20_1_MMStack_8.ome.tif
ln -s 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_9.ome.tif 01_638i100_640i150_405i50_x20_1_MMStack_9.ome.tif
ln -s 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack.ome.tif 01_638i100_640i150_405i50_x20_1_MMStack.ome.tif
cd /nfs/ebi/bioimage/drop/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-TPR
ln -s 02_638i100_640i150_405i50_x20_1_MMStack2_10.ome.tif 02_638i100_640i150_405i50_x20_1_MMStack_10.ome.tif
ln -s 02_638i100_640i150_405i50_x20_1_MMStack2_11.ome.tif 02_638i100_640i150_405i50_x20_1_MMStack_11.ome.tif
ln -s 02_638i100_640i150_405i50_x20_1_MMStack2_12.ome.tif 02_638i100_640i150_405i50_x20_1_MMStack_12.ome.tif
ln -s 02_638i100_640i150_405i50_x20_1_MMStack2_13.ome.tif 02_638i100_640i150_405i50_x20_1_MMStack_13.ome.tif
ln -s 02_638i100_640i150_405i50_x20_1_MMStack2_7.ome.tif 02_638i100_640i150_405i50_x20_1_MMStack_7.ome.tif
ln -s 02_638i100_640i150_405i50_x20_1_MMStack2_8.ome.tif 02_638i100_640i150_405i50_x20_1_MMStack_8.ome.tif
ln -s 02_638i100_640i150_405i50_x20_1_MMStack2_9.ome.tif 02_638i100_640i150_405i50_x20_1_MMStack_9.ome.tif

But the images are still broken. So I tried to reimport them, which also doesn't work. For example frist image:

Bioformats showinf doesn't have a problem:

>  BF_MAX_MEM=10G showinf -nopix /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack.ome.tif
Checking file format [OME-TIFF]
Initializing reader
OMETiffReader initializing /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack.ome.tif
Initialization took 95.05s

Reading core metadata
filename = /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack.ome.tif
Used files:
        /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_MMStack.ome.tif
        /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_MMStack_1.ome.tif
        /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_MMStack_2.ome.tif
        /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_MMStack_3.ome.tif
        /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_MMStack_4.ome.tif
        /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_MMStack_5.ome.tif
        /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_MMStack_6.ome.tif
        /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_MMStack_7.ome.tif
        /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_MMStack_8.ome.tif
        /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_MMStack_9.ome.tif
        /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_MMStack_10.ome.tif
        /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_MMStack_11.ome.tif
        /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_MMStack_12.ome.tif
        /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_MMStack_13.ome.tif
Series count = 1
Reading IFDs
Populating metadata
Series #0 :
        Image count = 171578
        RGB = false (1)
        Interleaved = false
        Indexed = false (false color)
        Width = 330
        Height = 512
        SizeZ = 1
        SizeT = 171578
        SizeC = 1
        Tile size = 336 x 512
        Thumbnail size = 82 x 128
        Endianness = intel (little)
        Dimension order = XYCZT (certain)
        Pixel type = uint16
        Valid bits per pixel = 16
        Metadata complete = true
        Thumbnail series = false
        -----
        Plane #0 <=> Z 0, C 0, T 0
        Plane #85787 <=> Z 0, C 0, T 85787
        Plane #85788 <=> Z 0, C 0, T 85788
        Plane #85789 <=> Z 0, C 0, T 85789
        Plane #85790 <=> Z 0, C 0, T 85790
        Plane #85791 <=> Z 0, C 0, T 85791
        Plane #171577 <=> Z 0, C 0, T 171577


Reading global metadata

Reading metadata

But omero import doesn't find a import:

>/opt/omero/server/OMERO.server/bin/omero import --transfer=ln_s /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack.ome.tif
Created session for demo@localhost:4064. Idle timeout: 10 min. Current group: Public
2025-04-08 13:29:34,594 2316       [      main] INFO          ome.formats.importer.ImportConfig - OMERO.blitz Version: 5.7.4
2025-04-08 13:29:34,630 2352       [      main] INFO          ome.formats.importer.ImportConfig - Bioformats version: 7.3.1 revision: 41e12f630ad7349caa0293cba23948c17d22732b date: 11 July 2024
2025-04-08 13:29:38,757 6479       [      main] INFO   formats.importer.cli.CommandLineImporter - Setting transfer to ln_s
2025-04-08 13:29:38,761 6483       [      main] INFO   formats.importer.cli.CommandLineImporter - Log levels -- Bio-Formats: ERROR OMERO.importer: INFO
2025-04-08 13:29:39,373 7095       [      main] INFO      ome.formats.importer.ImportCandidates - Depth: 4 Metadata Level: MINIMUM
2025-04-08 13:32:59,699 207421     [      main] INFO      ome.formats.importer.ImportCandidates - 1 file(s) parsed into 0 group(s) with 1 call(s) to setId in 200300ms. (200326ms total) [0 unknowns]
2025-04-08 13:32:59,777 207499     [      main] INFO       ome.formats.OMEROMetadataStoreClient - Attempting initial SSL connection to localhost:4064
2025-04-08 13:33:01,315 209037     [      main] INFO       ome.formats.OMEROMetadataStoreClient - Insecure connection requested, falling back
2025-04-08 13:33:01,627 209349     [      main] INFO       ome.formats.OMEROMetadataStoreClient - Pinging session every 300s.
2025-04-08 13:33:01,637 209359     [      main] INFO       ome.formats.OMEROMetadataStoreClient - Server: 5.6.13
2025-04-08 13:33:01,638 209360     [      main] INFO       ome.formats.OMEROMetadataStoreClient - Client: 5.7.4
2025-04-08 13:33:01,638 209360     [      main] INFO       ome.formats.OMEROMetadataStoreClient - Java Version: 23.0.2-internal
2025-04-08 13:33:01,638 209360     [      main] INFO       ome.formats.OMEROMetadataStoreClient - OS Name: Linux
2025-04-08 13:33:01,638 209360     [      main] INFO       ome.formats.OMEROMetadataStoreClient - OS Arch: amd64
2025-04-08 13:33:01,638 209360     [      main] INFO       ome.formats.OMEROMetadataStoreClient - OS Version: 5.14.0-503.26.1.el9_5.x86_64
No imports found

But all the files are there:

> ls -lah /uod/idr/filesets/idr0108-sabinina-nuclearporecomplex/20210426-Globus/SNAP-RANBP2/ | grep 01_638i100_640i150_405i50_x20_1_MM
lrwxrwxrwx.  1  11447 idr-users    73 Mar 24 10:45 01_638i100_640i150_405i50_x20_1_MMStack_10.ome.tif -> 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_10.ome.tif
lrwxrwxrwx.  1  11447 idr-users    73 Mar 24 10:45 01_638i100_640i150_405i50_x20_1_MMStack_11.ome.tif -> 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_11.ome.tif
lrwxrwxrwx.  1  11447 idr-users    73 Mar 24 10:45 01_638i100_640i150_405i50_x20_1_MMStack_12.ome.tif -> 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_12.ome.tif
lrwxrwxrwx.  1  11447 idr-users    73 Mar 24 10:45 01_638i100_640i150_405i50_x20_1_MMStack_13.ome.tif -> 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_13.ome.tif
-rw-rw----.  1 idrftp idr-users  4.1G Apr 28  2021 01_638i100_640i150_405i50_x20_1_MMStack_1.ome.tif
-rw-rw----.  1 idrftp idr-users  4.1G Apr 28  2021 01_638i100_640i150_405i50_x20_1_MMStack_2.ome.tif
-rw-rw----.  1 idrftp idr-users  4.1G Apr 28  2021 01_638i100_640i150_405i50_x20_1_MMStack_3.ome.tif
-rw-rw----.  1 idrftp idr-users  4.1G Apr 28  2021 01_638i100_640i150_405i50_x20_1_MMStack_4.ome.tif
-rw-rw----.  1 idrftp idr-users  4.1G Apr 28  2021 01_638i100_640i150_405i50_x20_1_MMStack_5.ome.tif
-rw-rw----.  1 idrftp idr-users  4.1G Apr 28  2021 01_638i100_640i150_405i50_x20_1_MMStack_6.ome.tif
-rw-rw----.  1 idrftp idr-users  3.6G Apr 29  2021 01_638i100_640i150_405i50_x20_1_MMStack_7.ome.tif
lrwxrwxrwx.  1  11447 idr-users    72 Mar 24 10:45 01_638i100_640i150_405i50_x20_1_MMStack_8.ome.tif -> 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_8.ome.tif
lrwxrwxrwx.  1  11447 idr-users    72 Mar 24 10:45 01_638i100_640i150_405i50_x20_1_MMStack_9.ome.tif -> 01_638i100_640i150_405i50_x20_1_locprec_10_z35_LL1-6_1_MMStack_9.ome.tif
-rw-rw----.  1 idrftp idr-users 1020M Apr 29  2021 01_638i100_640i150_405i50_x20_1_MMStack_metadata.txt
-rw-rw----.  1 idrftp idr-users  4.1G Apr 28  2021 01_638i100_640i150_405i50_x20_1_MMStack.ome.tif

/cc @sbesson Any more ideas?

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