- Download peak calling tool: https://github.com/FredHutch/SEACR
- Set tool path, project path, fastq path as following:
cores, projPath, fastqdir, seacr, trimmomaticpath, ref, spikeInRef, chromSize
- Submit the pipeline as following:
sbatch -p himem -J cutrun --export=ALL -N 1 -n 5 --mem 80G -t 1-0 --wrap "bash CUT-RUN.pipeline.sh K27me3"
Zheng Y et al (2020). Protocol.io