Protein intensities appear to depend on the order of ions

[mhamaneh]

Hi
I can run directlfq (using a generic input file) without a problem, but the resulting protein intensities change when I shuffle the lines in the input file that are related to the same protein. This sounds strange to me as the protein intensities should not depend on the order of ions related to the same protein. Could you please take look at this issue? I run directlfq within a python script using the following command:

lfq_manager.run_lfq("direct_lfq.aq_reformat.tsv")

Thank you for your help

[ammarcsj]

Hi, thanks a lot for making us aware of this. This was due to a sorting issue, we fixed this in the PR mentioned above that and will release it soon.

[mhamaneh]

Hi
Thank you for fixing the issue. When will the new version be released?

[ammarcsj]

Hi, the new version is up under "releases" :)

[mhamaneh]

OK, thank you very much.

[mhamaneh]

Hello again,

I installed version 0.3.1, but I am still observing the same strange behavior. Am I missing something?

Thanks

[mschwoer]

apologies, there was some internal miscommunication. The fix is in this branch: https://github.com/MannLabs/directlfq/tree/fix_intensity_issues , not part of 0.3.1, but will be release soon.