ZebraFET/CITATION.cff at main · MoschenHTS/ZebraFET · GitHub

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cff-version: 1.2.0
message: "If you use ZebraFET in your research, please cite it using the metadata below."
type: software
title: "ZebraFET — Standardized Zebrafish Embryo Toxicity Test Assistant (OECD TG 236)"
version: "2.2.0"
date-released: "2026-07-30"
doi: "10.5281/zenodo.20183712"
license: GPL-3.0
repository-code: "https://github.com/MoschenHTS/ZebraFET"
url: "https://doi.org/10.5281/zenodo.20183712"
authors:
  - family-names: Moschen
    given-names: Henrique Tamanini S.
    orcid: "https://orcid.org/0000-0002-1920-8915"
    email: henriquetamanini@icloud.com
    affiliation: "Toxinology Laboratory, Center for Molecular Biotechnology, University of Brasília (UnB), Brasília, DF, Brazil"
  - family-names: Zimerman
    given-names: Jeanini
    affiliation: "Laboratory of Bioenergetics and Metabolism, Department of Genetics and Morphology, Institute of Biology, University of Brasília (UnB), Brasília, DF, Brazil"
  - family-names: Matos
    given-names: Vinicius de Faria
    affiliation: "Laboratory of Bioenergetics and Metabolism, Department of Genetics and Morphology, Institute of Biology, University of Brasília (UnB), Brasília, DF, Brazil"
  - family-names: Liberal
    given-names: Clara Helena Cubas
    affiliation: "Laboratory of Bioenergetics and Metabolism, Department of Genetics and Morphology, Institute of Biology, University of Brasília (UnB), Brasília, DF, Brazil"
  - family-names: Bellozi
    given-names: Paula Maria Quaglio
    affiliation: "Laboratory of Bioenergetics and Metabolism, Department of Genetics and Morphology, Institute of Biology, University of Brasília (UnB), Brasília, DF, Brazil"
  - family-names: de Bem
    given-names: Andreza Fabro
    affiliation: "Brazilian National Institute of Science and Technology on Neuroimmunomodulation, Oswaldo Cruz Foundation, Rio de Janeiro, RJ, Brazil"
  - family-names: Goulart
    given-names: Jair Trapé
    affiliation: "Laboratory of Bioenergetics and Metabolism, Department of Genetics and Morphology, Institute of Biology, University of Brasília (UnB), Brasília, DF, Brazil"
keywords:
  - zebrafish
  - Danio rerio
  - fish embryo toxicity
  - FET test
  - OECD TG 236
  - ecotoxicology
  - aquatic toxicology
  - regulatory toxicology
  - LC50
  - NOEC
  - LOEC
  - dose-response
  - teratogenicity
  - sublethal endpoints
  - Python
  - desktop application
  - research software
abstract: "ZebraFET is a free, open-source desktop application for conducting, managing, and analyzing acute zebrafish (Danio rerio) embryo toxicity tests — the Fish Embryo Acute Toxicity (FET) test — following OECD Test Guideline 236. It covers the full experimental workflow in one interface: project setup and concentration planning, plate layout, daily well scoring, photo documentation, and water-quality logging. Analysis covers lethal endpoints (LC50 by logistic regression with bootstrap confidence intervals, a distribution-free trimmed Spearman-Karber LC50, the LC50 refitted at each daily timepoint, NOEC/LOEC with Holm-Bonferroni correction, and Cochran-Armitage trend tests) and sublethal ones (per-endpoint malformation testing with false-discovery-rate control, a sublethal NOEC/LOEC, and a teratogenic index), with odds ratios and Wilson confidence intervals throughout. Test validity is assessed against every OECD TG 236 Section 9 criterion the recorded data supports. Results export as CSV tables and as a formatted Word report. ZebraFET runs on Windows, macOS, and Linux, and is released under the GNU General Public License v3.0."