Values of the deconvolved trace in GUI vs in the spks.npy trace

[harshidapancholi]

Hello,

I have observed that the spks.npy values are scaled down from what I see in the GUI. For example, at a specific frame, the GUI shows a value of ~450, whereas the .npy file when loaded using Spyder shows a value of ~150. Is there a scaling factor somewhere of which I am unaware? How does that effect my interpretation of data? I work with very noisy (low res, sparse labeling) 2P imaging data.

Best,
Harshida