Describe the issue:
I was running into a Exception: Resource temporarily unavailable error when opening Bruker .ome.tif files on a compute cluster. I'm not sure why I was getting that error in the first place but this bug report is about my attempted workaround.
I tried to force suite2p to use tifffile.imread with force_sktiff = True however the same exception was generated the same way, so I think it is still trying to use scanimage-tiff-reader. I believe this line in tiff.ome_to_binary() may be the culprit, since it's just checking if scanimage-tiff-reader is installed and not regarding the db dictionary:
|
use_sktiff = not HAS_SCANIMAGE |
I'm not sure if a similar issue arises from other methods in the tiff module as well. I got around it by uninstalling scanimage-tiff-reader, and files are loading just fine using tifffile.
Reproduce the code example:
import suite2p
datadir = ...
database = {
'data_path': [datadir],
'input_format': 'bruker',
'nchannels': 1,
'nplanes': 1,
'batch_size': 2000,
'functional_chan': 2,
'force_sktiff': 1,
}
settings = suite2p.default_settings()
output_ops = suite2p.run_s2p(settings=settings, db=database)
Example input dataset used to reproduce the issue:
No response
Error message:
Traceback (most recent call last):
File "<stdin>", line 1, in <module>
File "/network/rit/lab/turner_lab/miniconda/envs/turnersuite/lib/python3.10/site-packages/suite2p/run_s2p.py", line 438, in run_s2p
dbs = files_to_binary[db["input_format"]](dbs, settings, files, files_chan2)
File "/network/rit/lab/turner_lab/miniconda/envs/turnersuite/lib/python3.10/site-packages/suite2p/io/tiff.py", line 396, in ome_to_binary
im = read_tiff(file, tif, Ltif, ix, batch_size, use_sktiff)
File "/network/rit/lab/turner_lab/miniconda/envs/turnersuite/lib/python3.10/site-packages/suite2p/io/tiff.py", line 126, in read_tiff
im = tif.data(beg=ix, end=ix + nfr)
File "/network/rit/lab/turner_lab/miniconda/envs/turnersuite/lib/python3.10/site-packages/ScanImageTiffReader/__init__.py", line 309, in data
raise Exception(self.__ctx.log.decode("utf-8","strict"))
Exception: Resource temporarily unavailable
Version information:
suite2p v1.1.0
Context for the issue:
No response
Describe the issue:
I was running into a
Exception: Resource temporarily unavailableerror when opening Bruker .ome.tif files on a compute cluster. I'm not sure why I was getting that error in the first place but this bug report is about my attempted workaround.I tried to force suite2p to use tifffile.imread with
force_sktiff = Truehowever the same exception was generated the same way, so I think it is still trying to use scanimage-tiff-reader. I believe this line in tiff.ome_to_binary() may be the culprit, since it's just checking if scanimage-tiff-reader is installed and not regarding the db dictionary:suite2p/suite2p/io/tiff.py
Line 323 in 90be895
I'm not sure if a similar issue arises from other methods in the tiff module as well. I got around it by uninstalling scanimage-tiff-reader, and files are loading just fine using tifffile.
Reproduce the code example:
Example input dataset used to reproduce the issue:
No response
Error message:
Version information:
suite2p v1.1.0
Context for the issue:
No response