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Merge pull request #1204 from NFDI4BIOIMAGE/git-bob-mod-1x3TnUwFOg
Add content from communities: nfdi4bioimage, gerbi, euro-bioimaging, neubias, bio-formats, globias, rdm4mic
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resources/nfdi4bioimage.yml

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url:
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- https://zenodo.org/records/19352622
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- https://doi.org/10.5281/zenodo.19352622
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- authors:
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- Wetzker, Cornelia
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- Ahmadi, Mohsen
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- "Kroo\xDF, Ksenia"
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- Wendt, Jens
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- Boissonnet, Tom
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description: 'The slides are part of a hands-on session that demonstrates the usage
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of OMERO.figure to create overviews of microscopy images and datasets presented
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at the Workshop Data Stewardship in Microscopy at the Trends in Microscopy 2025.
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Usage of the material in self learning:
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To be able to follow the hands-on material, find the 3 example datasets of the
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workshop openly accessible in a public user space of an OMERO instance at the
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University of Münster via this link: https://omero-tim.gerbi-gmb.de/webclient/?show=dataset-25739
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Alternatively, open the tool omero.figure in this instance directly (https://omero-tim.gerbi-gmb.de/figure/)
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and add one of the datasets by providing its id: 105986. (see the slides for details)
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Origin of the microscopy dataset:
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The datasets originate from a project dealing with fluorescence lifetime imaging
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microscopy (FLIM) and its possible application to distinguish spectrally overlapping
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fluorophores based on their lifetimes. The scientific context and further
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processing of the image datasets are described in the following publication: https://doi.org/10.1111/jmi.70036
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The complete bioimage dataset including experimental, microscopy and image analysis
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metadata can be found on the BioImage Archive: https://www.ebi.ac.uk/biostudies/bioimages/studies/S-BIAD1967'
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license: cc-by-4.0
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name: '[TiM2025] Hands-on: Creating Bio-image Overviews with OMERO.figure'
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num_downloads: 0
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publication_date: '2026-06-09'
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submission_date: '2026-06-09T13:45:54.139699'
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url:
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- https://zenodo.org/records/20610539
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- https://doi.org/10.5281/zenodo.20610539
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- authors:
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- Moore, Josh
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- Schmidt, Christian
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- Haase, Robert
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- Mallm, Jan-Philipp
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- Fortmann-Grote, Carsten
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- Figge, Thilo
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- Tischer, Christian
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- Sun, Yi
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- Zobel, Thomas
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- Wendt, Jens
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- "Gr\xFCning, Bj\xF6rn"
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- Kunis, Susanne
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- Blank-Burian, Markus
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- Trivedi, Ami
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- Hanne, Janina
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- von Suchodoletz, Dirk
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- Scherle, Michael
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- Massei, Riccardo
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- Wetzker, Cornelia
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- "M\xFCller, Maximilian"
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- Baldenius, Marie
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- Mohr, Inga
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- Fuchs, Vanessa
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- Gottschall, Tobias
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- Bortolomeazzi, Michele
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- Gerst, Ruman
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- Ahmadi, Mohsen
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- Chen, Jianxu
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- Becker, Markus
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- Ferrando-May, Elisa
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- Weidtkamp-Peters, Stefanie
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description: 'Interim Progress Report of the Consortium NFDI4BIOIMAGEPart 1 published
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at: https://www.dfg.de/en/research-funding/funding-initiative/nfdi/progress-reportsPart
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2 published here.
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Biological imaging is a core pillar of modern life science, enabling quantitative
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insight across scales and disciplines, from molecular biology to medicine. Increasingly,
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imaging is combined with other data modalities such as genomics and proteomics,
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positioning bioimaging at the heart of phenomics and integrative research. At
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the same time, bioimaging experiments are among the largest and most heterogeneous
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produced in academia, often surpassing terabyte scale. Without shared standards,
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scalable infrastructure, and coordinated community practices, much of this data
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risks remaining siloed or non-reusable, limiting its scientific value for the
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wider research community. NFDI4BIOIMAGE was established to address these
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challenges by embedding the German bioimaging community within a strong international
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ecosystem and contributing to long-standing global goals around data acquisition,
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management, discovery, and publication. Three years into its five-year funding
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period, NFDI4BIOIMAGE has become a visible and trusted actor in shaping how bioimaging
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data are standardized, shared, analyzed, and sustained, both nationally and internationally[1].
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At its inception, the consortium defined four closely linked objectives, spanning
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the full bioimaging data lifecycle and aligning national efforts with international
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developments (Figure 1).
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A central objective of NFDI4BIOIMAGE has been to champion open, community-driven
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standards (Obj. 1) that enable data to be reused across tools, institutions, and
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scientific domains. The consortium has led the development and international adoption
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of a Next-Generation File Format (NGFF) through a Request for Comments (RFC) process.
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This work directly addresses the needs of modern, cloud-scale, and multimodal
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imaging and has attracted sustained engagement from global partners, including
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follow-on funding from international sources such as Wellcome and Biohub. NGFF,
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however, goes beyond a file format, by enabling systematic extension in other
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ecosystems, like the emerging SpatialData standard for the specific needs of spatially
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resolved omics. This serves as a blueprint for domain-specific specialization
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from a shared core standard. In collaboration with the BioImage Archive (BIA)
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at EBI, the consortium is contributing to interoperable metadata specifications
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that move bioimaging closer to FAIR Digital Objects (FDOs), or “FAIR Image
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Objects” (FAIR-IO). Additional contributions include promotion of REMBI
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(Recommended Metadata for Biological Images) as a de facto metadata standard,
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RO-Crate–based packaging approaches, and expanding our data ambassador model
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into FAIR data champions who drive community adoption.
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To make standards usable in practice, NFDI4BIOIMAGE has invested heavily in scalable,
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interoperable infrastructure (Obj. 2) supporting both national and international
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workflows. This includes cloud-ready analysis environments based on Jupyter, object
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storage, Desktop-as-a-Service (DaaS) and workflow platforms such as Galaxy, enabling
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reproducible analysis of large imaging datasets across infrastructures. The consortium
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has supported the migration of major international resources, including the Image
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Data Resource (IDR), to scalable cloud-based access models and has worked closely
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with EMBL-EBI to convert BioImage Archive holdings into REMBI-annotated NGFFs,
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namely OME-Zarr, substantially improving online accessibility and reuse.
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At the software level, NFDI4BIOIMAGE has strengthened tools for maximizing reproducible
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bioimage analysis (Obj. 3), such as Galaxy and JIPipe, adding features including
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RO-Crate packaging, OME-Zarr support, and OMERO integration to bridge experimental
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analysis pipelines with FAIR data publication. The BioImage ANalysis Desktop (BAND)
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has served as a key transitional DaaS platform, bringing users’ experience
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closer to their large-scale imaging data in the cloud while maintaining an interactive,
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reproducible computational environment, which is particularly effective for training
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and lowering barriers to advanced analysis. The consortium has also engaged with
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emerging AI ecosystems like Hugging Face, aligning bioimaging workflows with broader
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developments in machine learning and reproducible research. In parallel, the consortium
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has supported local data stewardship and storage solutions at multiple German
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institutions and the operation of federated OMERO services, ensuring national
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capacity grows in step with international alignment.
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A defining strength of NFDI4BIOIMAGE is its emphasis on capacitating researchers
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through training and data stewardship (Obj. 4). Over the past three years, the
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consortium has delivered a broad program of training and capacity building, including
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institutional workshops, national courses, and contributing to major international
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conferences, and enhanced access to open educational resources for bioimaging
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RDM. Flagship initiatives such as RDM4mic and I3D:bio have significantly raised
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awareness and competence around research data management in bioimaging by giving
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the community a platform to actively engage. NFDI4BIOIMAGE has, furthermore, helped
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professionalize data stewardship roles through helpdesks and cross-consortia collaboration,
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supported data submission to the BioImage Archive, and improved discoverability
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through catalogs of tools, services, and use cases integrated into institutional
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research structures.
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In addition, NFDI4BIOIMAGE contributes actively beyond its consortium. The speaker
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has chaired the NFDI Konsortialversammlung while members participate broadly in
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relevant NFDI Sections, notably those focused on metadata (including ontologies,
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search, and knowledge graphs) and on common infrastructure and data integration.
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Internationally, consortium members contribute to ISO standardization, provide
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leadership within the Open Microscopy Environment (OME), and represent bioimaging
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interests in the European Open Science Cloud (EOSC). Engagement in initiatives
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such as IO-FAST and foundingGIDE, together with close ties to Global BioImaging,
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further amplify Germany’s visibility in the global imaging landscape. For
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the final two years of funding, NFDI4BIOIMAGE’s priorities include the formal
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standardization of widely adopted practices for bioimage data, establishment of
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a national OMERO framework in coordination with de.NBI (underpinned by an MoU),
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and the development of sustainable operational models for bioimaging data services.
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While challenges remain, particularly around long-term sustainability and scaling,
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the consortium has laid a robust foundation. By combining standards, infrastructure,
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tools, and people, NFDI4BIOIMAGE has positioned Germany as a central contributor
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to a FAIR, reproducible, globally connected bioimaging ecosystem.
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[1] https://youtu.be/7nj2BkRJ8Q0
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'
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license: cc-by-4.0
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name: NFDI4BIOIMAGE Interim Progress Report (03/2023 - 02/2026)
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num_downloads: 16
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publication_date: '2026-06-02'
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submission_date: '2026-06-09T13:45:54.584477'
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url:
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- https://zenodo.org/records/20509378
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- https://doi.org/10.5281/zenodo.20509378
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- authors:
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- Haase, Robert
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description: 'In  this presentation we learn  about the impact of generative
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artificial intelligence (AI), and in particular large language models (LLMs),
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on the field of bio image analysis. We dive deeper into data analysis code-generation
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and AI systems built around LLMs integrating code generation in our workflows
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conveniently:
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bia-bob
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git-bob
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sand-bob
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Finally, we take a look at how these systems can be used on sovereign infrastructure
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to support privacy in a scientific context.'
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license: cc-by-4.0
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name: How Generative Artificial Intelligence impacts Bio-image Data Science
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num_downloads: 182
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publication_date: '2026-06-02'
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submission_date: '2026-06-09T13:45:55.083802'
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url:
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- https://zenodo.org/records/20512957
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- https://doi.org/10.5281/zenodo.20512957
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- authors:
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- Moore, Josh
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description: '40 Minute talk presented to the BINA Insights and Educational Tools
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from Industry Series
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https://www.bioimagingnorthamerica.org/events/insights-and-educational-tools-from-industry-series-registration/'
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license: cc-by-4.0
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name: '[BINA] Understanding the Standardization Runway of OME-Zarr'
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num_downloads: 49
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publication_date: '2026-05-20'
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submission_date: '2026-06-09T13:45:55.545974'
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url:
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- https://zenodo.org/records/20275730
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- https://doi.org/10.5281/zenodo.20275730
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- authors:
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- Kemmer, Isabel
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- Euro-BioImaging ERIC
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description: 'Abstract: Euro-BioImaging Image Data Services includes the FAIR Data
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Toolkit to support its network of Nodes and their research communities in implementing
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robust bioimage data handling. This toolkit includes customizable data management
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plan templates, metadata & deposition guides, repository decision trees and
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a collection of training resources and consultation opportunities. All of these
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are designed to provide guidance around the entire bioimage data lifecycle, from
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acquisition to reuse. Beneath the surface of these resources, OME standards and
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tools provide the technical foundations for interoperability. Whether it’s
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OME metadata standards, Bio-Formats, OMERO, OME-Zarr, or the IDR, all of them
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are woven into the toolkit and shape our trainings and consultations. To enable
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broad adoption of OME-Zarr, we’re also creating open-source tools that streamline
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conversion and work with it. Through this OME integration, Euro-BioImaging turns
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FAIR community solutions into everyday practice by equipping Nodes with ready-to-use
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resources for their staff and researchers. Nodes adapt and distribute the toolkit
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locally, amplifying standardized, reproducible, and interoperable data handling
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across Europe’s bioimaging community.
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Note: this digital version of the poster was updated on 2026-05-27 with the latest
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numbers for Euro-BioImaging facilities and Image Data Community Days and is therefore
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slightly different than the one presented at the meeting.'
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license: cc-by-4.0
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name: '[OME2026] Poster:Putting FAIR Principles into Action - Euro-BioImaging''s
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FAIR Data Toolkit'
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num_downloads: 29
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publication_date: '2026-04-28'
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submission_date: '2026-06-09T13:46:20.979002'
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url:
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- https://zenodo.org/records/20152438
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- https://doi.org/10.5281/zenodo.20152438
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- authors:
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- Theart, Rensu
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- Levet, Florian
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- Hernandez-Herrera
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- Leterrier, Christophe
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- de la Ballina, Laura R
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description: 'Raw, processed and annotated images associated with the manuscript
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"Presynaptic Actin Nanostructures: A Reproducibility Case Study".
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This work is part of an initiative of the Global BioImage Analysts'' Society (GloBIAS)
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to assess reproducibility of published bioimage analysis workflows.
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We present our attempt to reproduce Figure 7 of the article Presynapses contain
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distinct actin nanostructures. J Cell Biol 2 October 2023; 222 (10): e202208110.
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doi: https://doi.org/10.1083/jcb.202208110. Original images and annotations
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were kindly provided by their authors.'
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license: cc-by-4.0
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name: 'Sample image data and annotations associated to Presynaptic Actin Nanostructures:
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A Reproducibility Case Study'
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num_downloads: 27
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publication_date: '2026-05-26'
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submission_date: '2026-06-09T13:47:01.007353'
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url:
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- https://zenodo.org/records/20386408
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- https://doi.org/10.5281/zenodo.20386408

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