@@ -27130,3 +27130,280 @@ resources:
2713027130 url:
2713127131 - https://zenodo.org/records/19352622
2713227132 - https://doi.org/10.5281/zenodo.19352622
27133+ - authors:
27134+ - Wetzker, Cornelia
27135+ - Ahmadi, Mohsen
27136+ - "Kroo\xDF, Ksenia"
27137+ - Wendt, Jens
27138+ - Boissonnet, Tom
27139+ description: 'The slides are part of a hands-on session that demonstrates the usage
27140+ of OMERO.figure to create overviews of microscopy images and datasets presented
27141+ at the Workshop Data Stewardship in Microscopy at the Trends in Microscopy 2025.
27142+
27143+ Usage of the material in self learning:
27144+
27145+ To be able to follow the hands-on material, find the 3 example datasets of the
27146+ workshop openly accessible in a public user space of an OMERO instance at the
27147+ University of Münster via this link: https://omero-tim.gerbi-gmb.de/webclient/?show=dataset-25739
27148+
27149+ Alternatively, open the tool omero.figure in this instance directly (https://omero-tim.gerbi-gmb.de/figure/)
27150+ and add one of the datasets by providing its id: 105986. (see the slides for details)
27151+
27152+ Origin of the microscopy dataset:
27153+
27154+ The datasets originate from a project dealing with fluorescence lifetime imaging
27155+ microscopy (FLIM) and its possible application to distinguish spectrally overlapping
27156+ fluorophores based on their lifetimes. The scientific context and further
27157+ processing of the image datasets are described in the following publication: https://doi.org/10.1111/jmi.70036
27158+
27159+ The complete bioimage dataset including experimental, microscopy and image analysis
27160+ metadata can be found on the BioImage Archive: https://www.ebi.ac.uk/biostudies/bioimages/studies/S-BIAD1967'
27161+ license: cc-by-4.0
27162+ name: '[TiM2025] Hands-on: Creating Bio-image Overviews with OMERO.figure'
27163+ num_downloads: 0
27164+ publication_date: '2026-06-09'
27165+ submission_date: '2026-06-09T13:45:54.139699'
27166+ url:
27167+ - https://zenodo.org/records/20610539
27168+ - https://doi.org/10.5281/zenodo.20610539
27169+ - authors:
27170+ - Moore, Josh
27171+ - Schmidt, Christian
27172+ - Haase, Robert
27173+ - Mallm, Jan-Philipp
27174+ - Fortmann-Grote, Carsten
27175+ - Figge, Thilo
27176+ - Tischer, Christian
27177+ - Sun, Yi
27178+ - Zobel, Thomas
27179+ - Wendt, Jens
27180+ - "Gr\xFCning, Bj\xF6rn"
27181+ - Kunis, Susanne
27182+ - Blank-Burian, Markus
27183+ - Trivedi, Ami
27184+ - Hanne, Janina
27185+ - von Suchodoletz, Dirk
27186+ - Scherle, Michael
27187+ - Massei, Riccardo
27188+ - Wetzker, Cornelia
27189+ - "M\xFCller, Maximilian"
27190+ - Baldenius, Marie
27191+ - Mohr, Inga
27192+ - Fuchs, Vanessa
27193+ - Gottschall, Tobias
27194+ - Bortolomeazzi, Michele
27195+ - Gerst, Ruman
27196+ - Ahmadi, Mohsen
27197+ - Chen, Jianxu
27198+ - Becker, Markus
27199+ - Ferrando-May, Elisa
27200+ - Weidtkamp-Peters, Stefanie
27201+ description: 'Interim Progress Report of the Consortium NFDI4BIOIMAGEPart 1 published
27202+ at: https://www.dfg.de/en/research-funding/funding-initiative/nfdi/progress-reportsPart
27203+ 2 published here.
27204+
27205+
27206+
27207+ Biological imaging is a core pillar of modern life science, enabling quantitative
27208+ insight across scales and disciplines, from molecular biology to medicine. Increasingly,
27209+ imaging is combined with other data modalities such as genomics and proteomics,
27210+ positioning bioimaging at the heart of phenomics and integrative research. At
27211+ the same time, bioimaging experiments are among the largest and most heterogeneous
27212+ produced in academia, often surpassing terabyte scale. Without shared standards,
27213+ scalable infrastructure, and coordinated community practices, much of this data
27214+ risks remaining siloed or non-reusable, limiting its scientific value for the
27215+ wider research community. NFDI4BIOIMAGE was established to address these
27216+ challenges by embedding the German bioimaging community within a strong international
27217+ ecosystem and contributing to long-standing global goals around data acquisition,
27218+ management, discovery, and publication. Three years into its five-year funding
27219+ period, NFDI4BIOIMAGE has become a visible and trusted actor in shaping how bioimaging
27220+ data are standardized, shared, analyzed, and sustained, both nationally and internationally[1].
27221+ At its inception, the consortium defined four closely linked objectives, spanning
27222+ the full bioimaging data lifecycle and aligning national efforts with international
27223+ developments (Figure 1).
27224+
27225+ A central objective of NFDI4BIOIMAGE has been to champion open, community-driven
27226+ standards (Obj. 1) that enable data to be reused across tools, institutions, and
27227+ scientific domains. The consortium has led the development and international adoption
27228+ of a Next-Generation File Format (NGFF) through a Request for Comments (RFC) process.
27229+ This work directly addresses the needs of modern, cloud-scale, and multimodal
27230+ imaging and has attracted sustained engagement from global partners, including
27231+ follow-on funding from international sources such as Wellcome and Biohub. NGFF,
27232+ however, goes beyond a file format, by enabling systematic extension in other
27233+ ecosystems, like the emerging SpatialData standard for the specific needs of spatially
27234+ resolved omics. This serves as a blueprint for domain-specific specialization
27235+ from a shared core standard. In collaboration with the BioImage Archive (BIA)
27236+ at EBI, the consortium is contributing to interoperable metadata specifications
27237+ that move bioimaging closer to FAIR Digital Objects (FDOs), or “FAIR Image
27238+ Objects” (FAIR-IO). Additional contributions include promotion of REMBI
27239+ (Recommended Metadata for Biological Images) as a de facto metadata standard,
27240+ RO-Crate–based packaging approaches, and expanding our data ambassador model
27241+ into FAIR data champions who drive community adoption.
27242+
27243+ To make standards usable in practice, NFDI4BIOIMAGE has invested heavily in scalable,
27244+ interoperable infrastructure (Obj. 2) supporting both national and international
27245+ workflows. This includes cloud-ready analysis environments based on Jupyter, object
27246+ storage, Desktop-as-a-Service (DaaS) and workflow platforms such as Galaxy, enabling
27247+ reproducible analysis of large imaging datasets across infrastructures. The consortium
27248+ has supported the migration of major international resources, including the Image
27249+ Data Resource (IDR), to scalable cloud-based access models and has worked closely
27250+ with EMBL-EBI to convert BioImage Archive holdings into REMBI-annotated NGFFs,
27251+ namely OME-Zarr, substantially improving online accessibility and reuse.
27252+
27253+ At the software level, NFDI4BIOIMAGE has strengthened tools for maximizing reproducible
27254+ bioimage analysis (Obj. 3), such as Galaxy and JIPipe, adding features including
27255+ RO-Crate packaging, OME-Zarr support, and OMERO integration to bridge experimental
27256+ analysis pipelines with FAIR data publication. The BioImage ANalysis Desktop (BAND)
27257+ has served as a key transitional DaaS platform, bringing users’ experience
27258+ closer to their large-scale imaging data in the cloud while maintaining an interactive,
27259+ reproducible computational environment, which is particularly effective for training
27260+ and lowering barriers to advanced analysis. The consortium has also engaged with
27261+ emerging AI ecosystems like Hugging Face, aligning bioimaging workflows with broader
27262+ developments in machine learning and reproducible research. In parallel, the consortium
27263+ has supported local data stewardship and storage solutions at multiple German
27264+ institutions and the operation of federated OMERO services, ensuring national
27265+ capacity grows in step with international alignment.
27266+
27267+ A defining strength of NFDI4BIOIMAGE is its emphasis on capacitating researchers
27268+ through training and data stewardship (Obj. 4). Over the past three years, the
27269+ consortium has delivered a broad program of training and capacity building, including
27270+ institutional workshops, national courses, and contributing to major international
27271+ conferences, and enhanced access to open educational resources for bioimaging
27272+ RDM. Flagship initiatives such as RDM4mic and I3D:bio have significantly raised
27273+ awareness and competence around research data management in bioimaging by giving
27274+ the community a platform to actively engage. NFDI4BIOIMAGE has, furthermore, helped
27275+ professionalize data stewardship roles through helpdesks and cross-consortia collaboration,
27276+ supported data submission to the BioImage Archive, and improved discoverability
27277+ through catalogs of tools, services, and use cases integrated into institutional
27278+ research structures.
27279+
27280+ In addition, NFDI4BIOIMAGE contributes actively beyond its consortium. The speaker
27281+ has chaired the NFDI Konsortialversammlung while members participate broadly in
27282+ relevant NFDI Sections, notably those focused on metadata (including ontologies,
27283+ search, and knowledge graphs) and on common infrastructure and data integration.
27284+ Internationally, consortium members contribute to ISO standardization, provide
27285+ leadership within the Open Microscopy Environment (OME), and represent bioimaging
27286+ interests in the European Open Science Cloud (EOSC). Engagement in initiatives
27287+ such as IO-FAST and foundingGIDE, together with close ties to Global BioImaging,
27288+ further amplify Germany’s visibility in the global imaging landscape. For
27289+ the final two years of funding, NFDI4BIOIMAGE’s priorities include the formal
27290+ standardization of widely adopted practices for bioimage data, establishment of
27291+ a national OMERO framework in coordination with de.NBI (underpinned by an MoU),
27292+ and the development of sustainable operational models for bioimaging data services.
27293+ While challenges remain, particularly around long-term sustainability and scaling,
27294+ the consortium has laid a robust foundation. By combining standards, infrastructure,
27295+ tools, and people, NFDI4BIOIMAGE has positioned Germany as a central contributor
27296+ to a FAIR, reproducible, globally connected bioimaging ecosystem.
27297+
27298+
27299+
27300+
27301+ [1] https://youtu.be/7nj2BkRJ8Q0
27302+
27303+
27304+ '
27305+ license: cc-by-4.0
27306+ name: NFDI4BIOIMAGE Interim Progress Report (03/2023 - 02/2026)
27307+ num_downloads: 16
27308+ publication_date: '2026-06-02'
27309+ submission_date: '2026-06-09T13:45:54.584477'
27310+ url:
27311+ - https://zenodo.org/records/20509378
27312+ - https://doi.org/10.5281/zenodo.20509378
27313+ - authors:
27314+ - Haase, Robert
27315+ description: 'In this presentation we learn about the impact of generative
27316+ artificial intelligence (AI), and in particular large language models (LLMs),
27317+ on the field of bio image analysis. We dive deeper into data analysis code-generation
27318+ and AI systems built around LLMs integrating code generation in our workflows
27319+ conveniently:
27320+
27321+
27322+ bia-bob
27323+
27324+ git-bob
27325+
27326+ sand-bob
27327+
27328+
27329+ Finally, we take a look at how these systems can be used on sovereign infrastructure
27330+ to support privacy in a scientific context.'
27331+ license: cc-by-4.0
27332+ name: How Generative Artificial Intelligence impacts Bio-image Data Science
27333+ num_downloads: 182
27334+ publication_date: '2026-06-02'
27335+ submission_date: '2026-06-09T13:45:55.083802'
27336+ url:
27337+ - https://zenodo.org/records/20512957
27338+ - https://doi.org/10.5281/zenodo.20512957
27339+ - authors:
27340+ - Moore, Josh
27341+ description: '40 Minute talk presented to the BINA Insights and Educational Tools
27342+ from Industry Series
27343+
27344+ https://www.bioimagingnorthamerica.org/events/insights-and-educational-tools-from-industry-series-registration/'
27345+ license: cc-by-4.0
27346+ name: '[BINA] Understanding the Standardization Runway of OME-Zarr'
27347+ num_downloads: 49
27348+ publication_date: '2026-05-20'
27349+ submission_date: '2026-06-09T13:45:55.545974'
27350+ url:
27351+ - https://zenodo.org/records/20275730
27352+ - https://doi.org/10.5281/zenodo.20275730
27353+ - authors:
27354+ - Kemmer, Isabel
27355+ - Euro-BioImaging ERIC
27356+ description: 'Abstract: Euro-BioImaging Image Data Services includes the FAIR Data
27357+ Toolkit to support its network of Nodes and their research communities in implementing
27358+ robust bioimage data handling. This toolkit includes customizable data management
27359+ plan templates, metadata & deposition guides, repository decision trees and
27360+ a collection of training resources and consultation opportunities. All of these
27361+ are designed to provide guidance around the entire bioimage data lifecycle, from
27362+ acquisition to reuse. Beneath the surface of these resources, OME standards and
27363+ tools provide the technical foundations for interoperability. Whether it’s
27364+ OME metadata standards, Bio-Formats, OMERO, OME-Zarr, or the IDR, all of them
27365+ are woven into the toolkit and shape our trainings and consultations. To enable
27366+ broad adoption of OME-Zarr, we’re also creating open-source tools that streamline
27367+ conversion and work with it. Through this OME integration, Euro-BioImaging turns
27368+ FAIR community solutions into everyday practice by equipping Nodes with ready-to-use
27369+ resources for their staff and researchers. Nodes adapt and distribute the toolkit
27370+ locally, amplifying standardized, reproducible, and interoperable data handling
27371+ across Europe’s bioimaging community.
27372+
27373+ Note: this digital version of the poster was updated on 2026-05-27 with the latest
27374+ numbers for Euro-BioImaging facilities and Image Data Community Days and is therefore
27375+ slightly different than the one presented at the meeting.'
27376+ license: cc-by-4.0
27377+ name: '[OME2026] Poster:Putting FAIR Principles into Action - Euro-BioImaging''s
27378+ FAIR Data Toolkit'
27379+ num_downloads: 29
27380+ publication_date: '2026-04-28'
27381+ submission_date: '2026-06-09T13:46:20.979002'
27382+ url:
27383+ - https://zenodo.org/records/20152438
27384+ - https://doi.org/10.5281/zenodo.20152438
27385+ - authors:
27386+ - Theart, Rensu
27387+ - Levet, Florian
27388+ - Hernandez-Herrera
27389+ - Leterrier, Christophe
27390+ - de la Ballina, Laura R
27391+ description: 'Raw, processed and annotated images associated with the manuscript
27392+ "Presynaptic Actin Nanostructures: A Reproducibility Case Study".
27393+
27394+ This work is part of an initiative of the Global BioImage Analysts'' Society (GloBIAS)
27395+ to assess reproducibility of published bioimage analysis workflows.
27396+
27397+ We present our attempt to reproduce Figure 7 of the article Presynapses contain
27398+ distinct actin nanostructures. J Cell Biol 2 October 2023; 222 (10): e202208110.
27399+ doi: https://doi.org/10.1083/jcb.202208110. Original images and annotations
27400+ were kindly provided by their authors.'
27401+ license: cc-by-4.0
27402+ name: 'Sample image data and annotations associated to Presynaptic Actin Nanostructures:
27403+ A Reproducibility Case Study'
27404+ num_downloads: 27
27405+ publication_date: '2026-05-26'
27406+ submission_date: '2026-06-09T13:47:01.007353'
27407+ url:
27408+ - https://zenodo.org/records/20386408
27409+ - https://doi.org/10.5281/zenodo.20386408
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