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Merge pull request #771 from NFDI4BIOIMAGE/haesleinhuepf-patch-6
removed duplicate
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resources/nfdi4bioimage.yml

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- https://zenodo.org/records/8252039
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- https://doi.org/10.5281/zenodo.8252039
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- authors:
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- Luke Sorensen
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- Ayame Saito
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- Sabrina Poon
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- Myat Noe Han
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- Adam Humenick
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- Peter Neckel
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- Keith Mutunduwe
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- Christie Glennan
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- Narges Mahdavian
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- Simon JH Brookes
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- Rachel M McQuade
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- Jaime PP Foong
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- Sebastian K. King
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- "Estibaliz G\xF3mez-de-Mariscal"
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- "Arrate Mu\xF1oz-Barrutia"
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- Robert Haase
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- Simona Carbone
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- Nicholas A. Veldhuis
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- Daniel P. Poole
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- Pradeep Rajasekhar
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description: "This upload is associated with the software, Gut Analysis Toolbox (GAT).\n\
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If you use it please cite:\nSorensen et al. Gut Analysis Toolbox: Automating\
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\ quantitative analysis of enteric neurons. J Cell Sci 2024; jcs.261950.\
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\ doi: https://doi.org/10.1242/jcs.261950\nThe upload contains StarDist models\
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\ for segmenting enteric neurons in 2D, enteric neuronal subtypes in 2D and FPN+ResNet101\
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\ model for enteric ganglia in 2D in gut wholemount tissue. GAT is implemented in\
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\ Fiji, but the models can be used in any software that supports StarDist and the\
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\ use of 2D UNet models. The files here also consist of Python notebooks\
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\ (Google Colab), training and test data as well as reports on model performance.\n\
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Note: The enteric ganglia model is has been updated to v3 which uses pytorch and\
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\ is a different architecture (FPN+ResNet101).\nThe model files are located in the\
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\ respective folders as zip files. The folders have also been zipped:\n\nNeuron\
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\ (Hu; StarDist model):\n\nMain folder: 2D_enteric_neuron_model_QA.zip\nStarDist\
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\ Model File:2D_enteric_neuron_v4_1.zip \nDeepImageJ compatible model: 2D_enteric_neuron.bioimage.io.model.zip\
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\ (used currently in GAT)\n\n\nNeuronal subtype (StarDist model): \n\n\
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Main folder: 2D_enteric_neuron_subtype_model_QA.zip\nModel File: 2D_enteric_neuron_subtype_v4.zip\n\
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DeepImageJ compatible model: 2D_enteric_neuron_subtype.bioimage.io.model.zip (used\
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\ currently in GAT)\n\n\nEnteric ganglia (2D FPN_ResNet101; Use in FIJI with deepImageJ)\n\
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\nMain folder: 2D_enteric_ganglia_v3_training.zip\nModel File: 2D_Ganglia_RGB_v3.bioimage.io.model.zip\
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\ (used currently in GAT)\n\n\n\nFor the all models, files included are:\n\nModel\
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\ for segmenting cells or ganglia in 2D FIJI. StarDist or 2D UNet.\nTraining and\
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\ Test datasets used for training.\nGoogle Colab notebooks used for training and\
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\ quality assurance (ZeroCost DL4Mic notebooks).\nPython notebook and code for training\
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\ ganglia model with QA.\nQuality assurance reports generated from above notebooks.\n\
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StarDist model exported for use in QuPath.\n\nThe model files can be used within\
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\ can be used within the software, StarDist. They are intended to be used\
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\ within FIJI or QuPath, but can be used in any software that supports the implementation\
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\ of StarDist in 2D.\nData:\nAll the images were collected from 4 different research\
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\ labs and a public database (SPARC database) to account for variations in image\
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\ acquisition, sample preparation and immunolabelling.\nFor enteric neurons the\
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\ pan-neuronal marker, Hu has been used and the  2D wholemounts images\
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\ from mouse, rat and human tissue.\nFor enteric neuronal subtypes, 2D images for\
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\ nNOS, MOR, DOR, ChAT, Calretinin, Calbindin, Neurofilament, CGRP and SST from\
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\ mouse tissue have been used..\n25 images were used from the following entries\
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\ in the SPARC database:\n\nHoward, M. (2021). 3D imaging of enteric neurons\
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\ in mouse (Version 1) [Data set]. SPARC Consortium. \nGraham, K. D., Huerta-Lopez,\
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\ S., Sengupta, R., Shenoy, A., Schneider, S., Wright, C. M., Feldman, M., Furth,\
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\ E., Lemke, A., Wilkins, B. J., Naji, A., Doolin, E., Howard, M., & Heuckeroth,\
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\ R. (2020). Robust 3-Dimensional visualization of human colon enteric nervous system\
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\ without tissue sectioning (Version 1) [Data set]. SPARC Consortium.\nWang, L.,\
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\ Yuan, P.-Q., Gould, T. and Tache, Y. (2021). Antibodies Tested in theColon –\
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\ Mouse (Version 1) [Data set]. SPARC Consortium. doi:10.26275/i7dl-58h\n\nAdditional\
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\ images for new ganglia model:\n\nHamnett, R., Dershowitz, L. B., Sampathkumar,\
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\ V., Wang, Z., Gomez-Frittelli, J., De Andrade, V., Kasthuri, N., Druckmann, S.\
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\ and Kaltschmidt, J. A. (2022b). Regional cytoarchitecture of the adult and developing\
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\ mouse enteric nervous system. Curr. Biol. 32, 4483-4492.e5.\n\nThe images have\
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\ been acquired using a combination different microscopes. The images for the mouse\
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\ tissue were acquired using: \n\n\nLeica TCS-SP8 confocal system (20x HC PL\
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\ APO NA 1.33, 40 x HC PL APO NA 1.3) \n\n\nLeica TCS-SP8 lightning confocal\
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\ system (20x HC PL APO NA 0.88) \n\n\nZeiss Axio Imager M2 (20X HC PL APO\
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\ NA 0.3) \n\n\nZeiss Axio Imager Z1 (10X HC PL APO NA 0.45) \n\n\nHuman\
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\ tissue images were acquired using: \n\n\nIX71 Olympus microscope (10X HC\
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\ PL APO NA 0.3) \n\n\nFor more information, visit the Documentation website.\n\
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NOTE: The images for enteric neurons and neuronal subtypes have been rescaled to\
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\ 0.568 µm/pixel for mouse and rat. For human neurons, it has been rescaled\
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\ to 0.9 µm/pixel . This is to ensure the neuronal cell bodies have similar\
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\ pixel area across images. The area of cells in pixels can vary based on resolution\
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\ of image, magnification of objective used, animal species (larger animals ->\
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\ larger neurons) and potentially how the tissue is stretched during wholemount\
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\ preparation \nAverage neuron area for neuronal model: 701.2 ±\
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\ 195.9 pixel2 (Mean ± SD, 6267 cells)\nAverage neuron area for neuronal\
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\ subtype model: 880.9 ± 316 pixel2 (Mean ± SD, 924 cells)\n\
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Software References:\nStardist\nSchmidt, U., Weigert, M., Broaddus, C., & Myers,\
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\ G. (2018, September). Cell detection with star-convex polygons. In International\
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\ Conference on Medical Image Computing and Computer-Assisted Intervention (pp.\
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\ 265-273). Springer, Cham.\ndeepImageJ\nGómez-de-Mariscal, E., García-López-de-Haro,\
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\ C., Ouyang, W., Donati, L., Lundberg, E., Unser, M., Muñoz-Barrutia, A.\
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\ and Sage, D., 2021. DeepImageJ: A user-friendly environment to run deep learning\
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\ models in ImageJ. Nature Methods, 18(10), pp.1192-1195.\nZeroCost DL4Mic\n\
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von Chamier, L., Laine, R.F., Jukkala, J., Spahn, C., Krentzel, D., Nehme, E., Lerche,\
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\ M., Hernández-Pérez, S., Mattila, P.K., Karinou, E. and Holden,\
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\ S., 2021. Democratising deep learning for microscopy with ZeroCostDL4Mic. Nature\
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\ communications, 12(1), pp.1-18."
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license: cc-by-4.0
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name: 'Gut Analysis Toolbox: Training data and 2D models for segmenting enteric neurons,
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neuronal subtypes and ganglia'
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num_downloads: 695
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publication_date: '2025-05-01'
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submission_date: '2025-05-29T19:38:39.675784'
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tags:
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- AI-ready
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type:
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- Data
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url:
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- https://zenodo.org/records/15314214
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- https://doi.org/10.5281/zenodo.15314214
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1306612958
- authors:
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- Johanna Jukkala
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- Guillaume Jacquemet

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