@@ -12955,114 +12955,6 @@ resources:
1295512955 - https://zenodo.org/records/8252039
1295612956 - https://doi.org/10.5281/zenodo.8252039
1295712957
12958- - authors:
12959- - Luke Sorensen
12960- - Ayame Saito
12961- - Sabrina Poon
12962- - Myat Noe Han
12963- - Adam Humenick
12964- - Peter Neckel
12965- - Keith Mutunduwe
12966- - Christie Glennan
12967- - Narges Mahdavian
12968- - Simon JH Brookes
12969- - Rachel M McQuade
12970- - Jaime PP Foong
12971- - Sebastian K. King
12972- - "Estibaliz G\xF3mez-de-Mariscal"
12973- - "Arrate Mu\xF1oz-Barrutia"
12974- - Robert Haase
12975- - Simona Carbone
12976- - Nicholas A. Veldhuis
12977- - Daniel P. Poole
12978- - Pradeep Rajasekhar
12979- description: "This upload is associated with the software, Gut Analysis Toolbox (GAT).\n\
12980- If you use it please cite:\nSorensen et al. Gut Analysis Toolbox: Automating\
12981- \ quantitative analysis of enteric neurons. J Cell Sci 2024; jcs.261950.\
12982- \ doi: https://doi.org/10.1242/jcs.261950\nThe upload contains StarDist models\
12983- \ for segmenting enteric neurons in 2D, enteric neuronal subtypes in 2D and FPN+ResNet101\
12984- \ model for enteric ganglia in 2D in gut wholemount tissue. GAT is implemented in\
12985- \ Fiji, but the models can be used in any software that supports StarDist and the\
12986- \ use of 2D UNet models. The files here also consist of Python notebooks\
12987- \ (Google Colab), training and test data as well as reports on model performance.\n\
12988- Note: The enteric ganglia model is has been updated to v3 which uses pytorch and\
12989- \ is a different architecture (FPN+ResNet101).\nThe model files are located in the\
12990- \ respective folders as zip files. The folders have also been zipped:\n\nNeuron\
12991- \ (Hu; StarDist model):\n\nMain folder: 2D_enteric_neuron_model_QA.zip\nStarDist\
12992- \ Model File:2D_enteric_neuron_v4_1.zip \nDeepImageJ compatible model: 2D_enteric_neuron.bioimage.io.model.zip\
12993- \ (used currently in GAT)\n\n\nNeuronal subtype (StarDist model): \n\n\
12994- Main folder: 2D_enteric_neuron_subtype_model_QA.zip\nModel File: 2D_enteric_neuron_subtype_v4.zip\n\
12995- DeepImageJ compatible model: 2D_enteric_neuron_subtype.bioimage.io.model.zip (used\
12996- \ currently in GAT)\n\n\nEnteric ganglia (2D FPN_ResNet101; Use in FIJI with deepImageJ)\n\
12997- \nMain folder: 2D_enteric_ganglia_v3_training.zip\nModel File: 2D_Ganglia_RGB_v3.bioimage.io.model.zip\
12998- \ (used currently in GAT)\n\n\n\nFor the all models, files included are:\n\nModel\
12999- \ for segmenting cells or ganglia in 2D FIJI. StarDist or 2D UNet.\nTraining and\
13000- \ Test datasets used for training.\nGoogle Colab notebooks used for training and\
13001- \ quality assurance (ZeroCost DL4Mic notebooks).\nPython notebook and code for training\
13002- \ ganglia model with QA.\nQuality assurance reports generated from above notebooks.\n\
13003- StarDist model exported for use in QuPath.\n\nThe model files can be used within\
13004- \ can be used within the software, StarDist. They are intended to be used\
13005- \ within FIJI or QuPath, but can be used in any software that supports the implementation\
13006- \ of StarDist in 2D.\nData:\nAll the images were collected from 4 different research\
13007- \ labs and a public database (SPARC database) to account for variations in image\
13008- \ acquisition, sample preparation and immunolabelling.\nFor enteric neurons the\
13009- \ pan-neuronal marker, Hu has been used and the 2D wholemounts images\
13010- \ from mouse, rat and human tissue.\nFor enteric neuronal subtypes, 2D images for\
13011- \ nNOS, MOR, DOR, ChAT, Calretinin, Calbindin, Neurofilament, CGRP and SST from\
13012- \ mouse tissue have been used..\n25 images were used from the following entries\
13013- \ in the SPARC database:\n\nHoward, M. (2021). 3D imaging of enteric neurons\
13014- \ in mouse (Version 1) [Data set]. SPARC Consortium. \nGraham, K. D., Huerta-Lopez,\
13015- \ S., Sengupta, R., Shenoy, A., Schneider, S., Wright, C. M., Feldman, M., Furth,\
13016- \ E., Lemke, A., Wilkins, B. J., Naji, A., Doolin, E., Howard, M., & Heuckeroth,\
13017- \ R. (2020). Robust 3-Dimensional visualization of human colon enteric nervous system\
13018- \ without tissue sectioning (Version 1) [Data set]. SPARC Consortium.\nWang, L.,\
13019- \ Yuan, P.-Q., Gould, T. and Tache, Y. (2021). Antibodies Tested in theColon –\
13020- \ Mouse (Version 1) [Data set]. SPARC Consortium. doi:10.26275/i7dl-58h\n\nAdditional\
13021- \ images for new ganglia model:\n\nHamnett, R., Dershowitz, L. B., Sampathkumar,\
13022- \ V., Wang, Z., Gomez-Frittelli, J., De Andrade, V., Kasthuri, N., Druckmann, S.\
13023- \ and Kaltschmidt, J. A. (2022b). Regional cytoarchitecture of the adult and developing\
13024- \ mouse enteric nervous system. Curr. Biol. 32, 4483-4492.e5.\n\nThe images have\
13025- \ been acquired using a combination different microscopes. The images for the mouse\
13026- \ tissue were acquired using: \n\n\nLeica TCS-SP8 confocal system (20x HC PL\
13027- \ APO NA 1.33, 40 x HC PL APO NA 1.3) \n\n\nLeica TCS-SP8 lightning confocal\
13028- \ system (20x HC PL APO NA 0.88) \n\n\nZeiss Axio Imager M2 (20X HC PL APO\
13029- \ NA 0.3) \n\n\nZeiss Axio Imager Z1 (10X HC PL APO NA 0.45) \n\n\nHuman\
13030- \ tissue images were acquired using: \n\n\nIX71 Olympus microscope (10X HC\
13031- \ PL APO NA 0.3) \n\n\nFor more information, visit the Documentation website.\n\
13032- NOTE: The images for enteric neurons and neuronal subtypes have been rescaled to\
13033- \ 0.568 µm/pixel for mouse and rat. For human neurons, it has been rescaled\
13034- \ to 0.9 µm/pixel . This is to ensure the neuronal cell bodies have similar\
13035- \ pixel area across images. The area of cells in pixels can vary based on resolution\
13036- \ of image, magnification of objective used, animal species (larger animals ->\
13037- \ larger neurons) and potentially how the tissue is stretched during wholemount\
13038- \ preparation \nAverage neuron area for neuronal model: 701.2 ±\
13039- \ 195.9 pixel2 (Mean ± SD, 6267 cells)\nAverage neuron area for neuronal\
13040- \ subtype model: 880.9 ± 316 pixel2 (Mean ± SD, 924 cells)\n\
13041- Software References:\nStardist\nSchmidt, U., Weigert, M., Broaddus, C., & Myers,\
13042- \ G. (2018, September). Cell detection with star-convex polygons. In International\
13043- \ Conference on Medical Image Computing and Computer-Assisted Intervention (pp.\
13044- \ 265-273). Springer, Cham.\ndeepImageJ\nGómez-de-Mariscal, E., García-López-de-Haro,\
13045- \ C., Ouyang, W., Donati, L., Lundberg, E., Unser, M., Muñoz-Barrutia, A.\
13046- \ and Sage, D., 2021. DeepImageJ: A user-friendly environment to run deep learning\
13047- \ models in ImageJ. Nature Methods, 18(10), pp.1192-1195.\nZeroCost DL4Mic\n\
13048- von Chamier, L., Laine, R.F., Jukkala, J., Spahn, C., Krentzel, D., Nehme, E., Lerche,\
13049- \ M., Hernández-Pérez, S., Mattila, P.K., Karinou, E. and Holden,\
13050- \ S., 2021. Democratising deep learning for microscopy with ZeroCostDL4Mic. Nature\
13051- \ communications, 12(1), pp.1-18."
13052- license: cc-by-4.0
13053- name: 'Gut Analysis Toolbox: Training data and 2D models for segmenting enteric neurons,
13054- neuronal subtypes and ganglia'
13055- num_downloads: 695
13056- publication_date: '2025-05-01'
13057- submission_date: '2025-05-29T19:38:39.675784'
13058- tags:
13059- - AI-ready
13060- type:
13061- - Data
13062- url:
13063- - https://zenodo.org/records/15314214
13064- - https://doi.org/10.5281/zenodo.15314214
13065-
1306612958- authors:
1306712959 - Johanna Jukkala
1306812960 - Guillaume Jacquemet
0 commit comments