diff --git a/.github/workflows/configurations/matlab_release_matrix_strategy.yml b/.github/workflows/configurations/matlab_release_matrix_strategy.yml
index caf18bcad..d0a1e5b12 100644
--- a/.github/workflows/configurations/matlab_release_matrix_strategy.yml
+++ b/.github/workflows/configurations/matlab_release_matrix_strategy.yml
@@ -21,4 +21,10 @@
   skip-pynwb-compatibilty-test-for-tutorial: '0'
 - matlab-version: R2024b
   python-version: '3.11'
+  skip-pynwb-compatibilty-test-for-tutorial: '0'
+- matlab-version: R2025a
+  python-version: '3.12'
+  skip-pynwb-compatibilty-test-for-tutorial: '0'
+- matlab-version: R2025b
+  python-version: '3.13'
   skip-pynwb-compatibilty-test-for-tutorial: '0'
\ No newline at end of file
diff --git a/.github/workflows/prepare_release.yml b/.github/workflows/prepare_release.yml
index 3fbbad163..08193d1ba 100644
--- a/.github/workflows/prepare_release.yml
+++ b/.github/workflows/prepare_release.yml
@@ -56,6 +56,12 @@ jobs:
           - matlab-version: R2024b
             python-version: '3.11'
             skip-pynwb-compatibilty-test-for-tutorial: '0'
+          - matlab-version: R2025a
+            python-version: '3.12'
+            skip-pynwb-compatibilty-test-for-tutorial: '0'
+          - matlab-version: R2025b
+            python-version: '3.13'
+            skip-pynwb-compatibilty-test-for-tutorial: '0'
     steps:
       - name: Check out repository
         uses: actions/checkout@v4
diff --git a/.github/workflows/run_tests.yml b/.github/workflows/run_tests.yml
index 9d5ce308b..0b7a42e77 100644
--- a/.github/workflows/run_tests.yml
+++ b/.github/workflows/run_tests.yml
@@ -9,6 +9,7 @@ on:
       - "*.md"
       - "*.codespellrc"
       - ".github/**"
+      - "!.github/workflows/run_tests.yml"
       - "docs/**"
   push:
     branches: