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关于step3的报错 #3

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@kikokhalifa

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@kikokhalifa

您好,在使用Rscript /home/bin/singlecell.r -i /mnt/d/scTEST/config.ini 命令后,出现以下报错。
请问该如何解决呢
2023-12-17 10:11:41 INFO::savefig at /mnt/d/outfile/step3/umap/plotby_ident
2023-12-17 10:11:43 INFO::savefig at /mnt/d/outfile/step3/umap/plotall_ident
Error in geom_point():
! Problem while computing aesthetics.
ℹ Error occurred in the 1st layer.
Caused by error in FUN():
! object 'UMAP_1' not found
Backtrace:

  1. ├─global savepdf(...)
  2. │ └─ggplot2::ggsave(...)
  3. │ ├─grid::grid.draw(plot)
  4. │ └─ggplot2:::grid.draw.ggplot(plot)
  5. │ ├─base::print(x)
  6. │ └─ggplot2:::print.ggplot(x)
  7. │ ├─ggplot2::ggplot_build(x)
  8. │ └─ggplot2:::ggplot_build.ggplot(x)
  9. │ └─ggplot2:::by_layer(...)
  10. │ ├─rlang::try_fetch(...)
  11. │ │ ├─base::tryCatch(...)
  12. │ │ │ └─base (local) tryCatchList(expr, classes, parentenv, handlers)
  13. │ │ │ └─base (local) tryCatchOne(expr, names, parentenv, handlers[[1L]])
  14. │ │ │ └─base (local) doTryCatch(return(expr), name, parentenv, handler)
  15. │ │ └─base::withCallingHandlers(...)
  16. │ └─ggplot2 (local) f(l = layers[[i]], d = data[[i]])
  17. │ └─l$compute_aesthetics(d, plot)
  18. │ └─ggplot2 (local) compute_aesthetics(..., self = self)
  19. │ └─ggplot2:::scales_add_defaults(...)
  20. │ └─base::lapply(aesthetics[new_aesthetics], eval_tidy, data = data)
  21. │ └─rlang (local) FUN(X[[i]], ...)
  22. └─base::.handleSimpleError(...)
  23. └─rlang (local) h(simpleError(msg, call))
  24. └─handlers[[1L]](cnd)
    
  25.   └─cli::cli_abort(...)
    
  26.     └─rlang::abort(...)
    

Warning messages:
1: In dir.create(paste(outdir, "step3", sep = "/"), recursive = T) :
'/mnt/d/outfile/step3' already exists
2: In dir.create(paste(outdir, "step3", "pca", sep = "/")) :
'/mnt/d/outfile/step3/pca' already exists
3: In ElbowPlot(pbmcall, csc$step3$elbowdims) :
The object only has information for 50 reductions
4: In dir.create(paste(outdir, "step3", cscumap, sep = "/")) :
'/mnt/d/outfile/step3/umap' already exists
Execution halted

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