test(analyses snapshots): partial tip protocols

analyses-snapshot-testing/files/protocols/Flex_S_2_20_96_None_COLUMN_HappyPath.py

@@ -0,0 +1,121 @@
+from opentrons.protocol_api import COLUMN
+
+metadata = {
+    "protocolName": "96Channel COLUMN Happy Path",
+    "description": "96 channel pipette and a COLUMN partial tip configuration.",
+}
+
+requirements = {
+    "robotType": "Flex",
+    "apiLevel": "2.20",
+}
+
+
+def run(protocol):
+
+    trash = protocol.load_trash_bin("A3")  # must load trash bin
+
+    partial_tip_rack = protocol.load_labware(
+        load_name="opentrons_flex_96_tiprack_1000ul",
+        label="Partial Tip Rack",
+        location="B2",
+    )
+
+    pipette = protocol.load_instrument(instrument_name="flex_96channel_1000")
+
+    # Which nozzle to use on the pipette
+    # Think of the nozzles from above like you are looking down on a 96-well plate
+    # start="A1" Means the West most nozzle column will pickup from the East most column of the tip rack
+    # start="A12" Means the East most nozzle column will pickup from the West most column of the tip rack
+    pipette.configure_nozzle_layout(
+        style=COLUMN,
+        start="A1",
+        tip_racks=[partial_tip_rack],
+    )
+
+    source_labware = protocol.load_labware(
+        load_name="nest_96_wellplate_100ul_pcr_full_skirt",
+        label="Liquid Transfer - Source Labware",
+        location="C2",
+    )
+
+    destination_labware = protocol.load_labware(
+        load_name="nest_96_wellplate_100ul_pcr_full_skirt",
+        label="Liquid Transfer - Destination Labware",
+        location="C3",
+    )
+
+    volume = 10  # Default volume for actions that require it
+
+    #############################
+    # Pipette do work
+
+    # consolidate does not work with the COLUMN configuration
+    # consolidate only has a single well as a destination
+
+    # transfer the row
+    pipette.transfer(volume, source_labware["A1"], destination_labware["A1"])
+
+    # distribute does not work with the COLUMN configuration
+    # distribute only has a single well as a source
+
+    pipette.pick_up_tip()
+    pipette.touch_tip(source_labware["A2"])
+    pipette.drop_tip()
+    pipette.pick_up_tip()
+    pipette.home()
+    pipette.drop_tip()
+
+    pipette.pick_up_tip()
+    well = source_labware["A3"]
+    # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate
+    pipette.move_to(well.bottom(z=2))
+    pipette.mix(10, 10)
+    pipette.move_to(well.top(z=5))
+    pipette.blow_out()
+    pipette.prepare_to_aspirate()
+    pipette.move_to(well.bottom(z=2))
+    pipette.aspirate(10, well.bottom(z=2))
+    pipette.dispense(10)
+    pipette.drop_tip()
+
+    #############################
+    # Change the pipette configuration
+
+    pipette.configure_nozzle_layout(
+        style=COLUMN,
+        start="A12",  # Which nozzle to start with
+        tip_racks=[partial_tip_rack],
+    )
+
+    #############################
+    # Pipette do work
+
+    # consolidate does not work with the COLUMN configuration
+    # consolidate only has a single well as a destination
+
+    # transfer the row
+    pipette.transfer(volume, source_labware["A5"], destination_labware["A5"])
+
+    # distribute does not work with the COLUMN configuration
+    # distribute only has a single well as a source
+
+    pipette.pick_up_tip()
+    pipette.touch_tip(source_labware["A6"])
+    pipette.drop_tip()
+    pipette.pick_up_tip()
+    pipette.home()
+    pipette.drop_tip()
+
+    pipette.pick_up_tip()
+    well = source_labware["A7"]
+    # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate
+    pipette.move_to(well.bottom(z=2))
+    pipette.mix(10, 10)
+    pipette.move_to(well.top(z=5))
+    pipette.blow_out()
+    pipette.prepare_to_aspirate()
+    pipette.move_to(well.bottom(z=2))
+    pipette.aspirate(10, well.bottom(z=2))
+    pipette.dispense(10)
+    pipette.drop_tip()

analyses-snapshot-testing/files/protocols/Flex_S_2_20_96_None_PARTIAL_COLUMN_HappyPath.py

@@ -0,0 +1,125 @@
+from opentrons.protocol_api import PARTIAL_COLUMN
+
+metadata = {
+    "protocolName": "96Channel PARTIAL_COLUMN Happy Path",
+    "description": "96 channel pipette and a PARTIAL_COLUMN partial tip configuration.",
+}
+
+requirements = {
+    "robotType": "Flex",
+    "apiLevel": "2.20",
+}
+
+
+def run(protocol):
+
+    trash = protocol.load_trash_bin("A3")  # must load trash bin
+
+    partial_tip_rack = protocol.load_labware(
+        load_name="opentrons_flex_96_tiprack_1000ul",
+        label="Partial Tip Rack",
+        location="D2",
+    )
+
+    pipette = protocol.load_instrument(instrument_name="flex_96channel_1000")
+
+    # Which nozzle to use on the pipette
+    # Think of the nozzles from above like you are looking down on a 96-well plate
+    # Picking up partial columns with the backmost nozzles is currently not supported. Setting
+    # ``style=PARTIAL_COLUMN`` and either ``start="A1"`` or ``start="A12"`` will raise an error.
+    # H1 means pipette overhang NE
+    # H12 means pipette overhang NW
+    pipette.configure_nozzle_layout(
+        style=PARTIAL_COLUMN,
+        start="H1",
+        end="D1",  # pick up the first 5
+        tip_racks=[partial_tip_rack],
+    )
+
+    source_labware = protocol.load_labware(
+        load_name="nest_96_wellplate_100ul_pcr_full_skirt",
+        label="Liquid Transfer - Source Labware",
+        location="C2",
+    )
+
+    destination_labware = protocol.load_labware(
+        load_name="nest_96_wellplate_100ul_pcr_full_skirt",
+        label="Liquid Transfer - Destination Labware",
+        location="C3",
+    )
+
+    volume = 10  # Default volume for actions that require it
+
+    #############################
+    # Pipette do work
+
+    # consolidate does not work with the PARTIAL_COLUMN configuration
+    # consolidate only has a single well as a destination
+
+    # transfer the row
+    pipette.transfer(volume, source_labware["D1"], destination_labware["D1"])
+
+    # distribute does not work with the PARTIAL_COLUMN configuration
+    # distribute only has a single well as a source
+
+    pipette.pick_up_tip()
+    pipette.touch_tip(source_labware["D2"])
+    pipette.drop_tip()
+    pipette.pick_up_tip()
+    pipette.home()
+    pipette.drop_tip()
+
+    pipette.pick_up_tip()
+    well = source_labware["D3"]
+    # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate
+    pipette.move_to(well.bottom(z=2))
+    pipette.mix(10, 10)
+    pipette.move_to(well.top(z=5))
+    pipette.blow_out()
+    pipette.prepare_to_aspirate()
+    pipette.move_to(well.bottom(z=2))
+    pipette.aspirate(10, well.bottom(z=2))
+    pipette.dispense(10)
+    pipette.drop_tip()
+
+    #############################
+    # Change the pipette configuration
+
+    pipette.configure_nozzle_layout(
+        style=PARTIAL_COLUMN,
+        start="H1",  # Which nozzle to start with
+        end="G1",  # 2 - the remaining 2 in the column
+        tip_racks=[partial_tip_rack],
+    )
+
+    #############################
+    # Pipette do work
+
+    # consolidate does not work with the PARTIAL_COLUMN configuration
+    # consolidate only has a single well as a destination
+
+    # transfer the row
+    pipette.transfer(volume, source_labware["B5"], destination_labware["B5"])
+
+    # distribute does not work with the PARTIAL_COLUMN configuration
+    # distribute only has a single well as a source
+
+    pipette.pick_up_tip()
+    pipette.touch_tip(source_labware["B6"])
+    pipette.drop_tip()
+    pipette.pick_up_tip()
+    pipette.home()
+    pipette.drop_tip()
+
+    pipette.pick_up_tip()
+    well = source_labware["B7"]
+    # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate
+    pipette.move_to(well.bottom(z=2))
+    pipette.mix(10, 10)
+    pipette.move_to(well.top(z=5))
+    pipette.blow_out()
+    pipette.prepare_to_aspirate()
+    pipette.move_to(well.bottom(z=2))
+    pipette.aspirate(10, well.bottom(z=2))
+    pipette.dispense(10)
+    pipette.drop_tip()

analyses-snapshot-testing/files/protocols/Flex_S_2_20_96_None_ROW_HappyPath.py

@@ -0,0 +1,121 @@
+from opentrons.protocol_api import ROW
+
+metadata = {
+    "protocolName": "96Channel ROW Happy Path",
+    "description": "96 channel pipette and a ROW partial tip configuration.",
+}
+
+requirements = {
+    "robotType": "Flex",
+    "apiLevel": "2.20",
+}
+
+
+def run(protocol):
+
+    trash = protocol.load_trash_bin("A3")  # must load trash bin
+
+    partial_tip_rack = protocol.load_labware(
+        load_name="opentrons_flex_96_tiprack_1000ul",
+        label="Partial Tip Rack",
+        location="B1",
+    )
+
+    pipette = protocol.load_instrument(instrument_name="flex_96channel_1000")
+
+    # Which nozzle to use on the pipette
+    # Think of the nozzles from above like you are looking down on a 96-well plate
+    # start="A1" Means the North most nozzle row will pickup from the South most row of the tip rack
+    # start="H1" Means the South most nozzle row will pickup from the North most row of the tip rack
+    pipette.configure_nozzle_layout(
+        style=ROW,
+        start="A1",
+        tip_racks=[partial_tip_rack],
+    )
+
+    source_labware = protocol.load_labware(
+        load_name="nest_96_wellplate_100ul_pcr_full_skirt",
+        label="Liquid Transfer - Source Labware",
+        location="C2",
+    )
+
+    destination_labware = protocol.load_labware(
+        load_name="nest_96_wellplate_100ul_pcr_full_skirt",
+        label="Liquid Transfer - Destination Labware",
+        location="C3",
+    )
+
+    volume = 10  # Default volume for actions that require it
+
+    #############################
+    # Pipette do work
+
+    # consolidate is does not work with the ROW configuration
+    # consolidate only has a single well as a destination
+
+    # transfer the row
+    pipette.transfer(volume, source_labware["A1"], destination_labware["A1"])
+
+    # distribute does not work with the ROW configuration
+    # distribute only has a single well as a source
+
+    pipette.pick_up_tip()
+    pipette.touch_tip(source_labware["B1"])
+    pipette.drop_tip()
+    pipette.pick_up_tip()
+    pipette.home()
+    pipette.drop_tip()
+
+    pipette.pick_up_tip()
+    well = source_labware["D1"]
+    # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate
+    pipette.move_to(well.bottom(z=2))
+    pipette.mix(10, 10)
+    pipette.move_to(well.top(z=5))
+    pipette.blow_out()
+    pipette.prepare_to_aspirate()
+    pipette.move_to(well.bottom(z=2))
+    pipette.aspirate(10, well.bottom(z=2))
+    pipette.dispense(10)
+    pipette.drop_tip()
+
+    #############################
+    # Change the pipette configuration
+
+    pipette.configure_nozzle_layout(
+        style=ROW,
+        start="H1",  # Which nozzle to start with
+        tip_racks=[partial_tip_rack],
+    )
+
+    #############################
+    # Pipette do work
+
+    # consolidate is does not work with the ROW configuration
+    # consolidate only has a single well as a destination
+
+    # transfer the row
+    pipette.transfer(volume, source_labware["E1"], destination_labware["E1"])
+
+    # distribute does not work with the ROW configuration
+    # distribute only has a single well as a source
+
+    pipette.pick_up_tip()
+    pipette.touch_tip(source_labware["F1"])
+    pipette.drop_tip()
+    pipette.pick_up_tip()
+    pipette.home()
+    pipette.drop_tip()
+
+    pipette.pick_up_tip()
+    well = source_labware["G1"]
+    # directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate
+    pipette.move_to(well.bottom(z=2))
+    pipette.mix(10, 10)
+    pipette.move_to(well.top(z=5))
+    pipette.blow_out()
+    pipette.prepare_to_aspirate()
+    pipette.move_to(well.bottom(z=2))
+    pipette.aspirate(10, well.bottom(z=2))
+    pipette.dispense(10)
+    pipette.drop_tip()