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test(analyses snapshots): partial tip protocols
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‎analyses-snapshot-testing/files/protocols/Flex_S_2_20_96_None_COLUMN_HappyPath.py

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from opentrons.protocol_api import COLUMN
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metadata = {
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"protocolName": "96Channel COLUMN Happy Path",
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"description": "96 channel pipette and a COLUMN partial tip configuration.",
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}
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requirements = {
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"robotType": "Flex",
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"apiLevel": "2.20",
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}
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def run(protocol):
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trash = protocol.load_trash_bin("A3") # must load trash bin
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partial_tip_rack = protocol.load_labware(
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load_name="opentrons_flex_96_tiprack_1000ul",
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label="Partial Tip Rack",
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location="B2",
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)
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pipette = protocol.load_instrument(instrument_name="flex_96channel_1000")
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# Which nozzle to use on the pipette
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# Think of the nozzles from above like you are looking down on a 96-well plate
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# start="A1" Means the West most nozzle column will pickup from the East most column of the tip rack
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# start="A12" Means the East most nozzle column will pickup from the West most column of the tip rack
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pipette.configure_nozzle_layout(
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style=COLUMN,
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start="A1",
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tip_racks=[partial_tip_rack],
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)
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source_labware = protocol.load_labware(
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load_name="nest_96_wellplate_100ul_pcr_full_skirt",
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label="Liquid Transfer - Source Labware",
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location="C2",
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)
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destination_labware = protocol.load_labware(
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load_name="nest_96_wellplate_100ul_pcr_full_skirt",
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label="Liquid Transfer - Destination Labware",
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location="C3",
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)
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volume = 10 # Default volume for actions that require it
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#############################
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# Pipette do work
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# consolidate does not work with the COLUMN configuration
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# consolidate only has a single well as a destination
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# transfer the row
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pipette.transfer(volume, source_labware["A1"], destination_labware["A1"])
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# distribute does not work with the COLUMN configuration
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# distribute only has a single well as a source
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pipette.pick_up_tip()
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pipette.touch_tip(source_labware["A2"])
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pipette.drop_tip()
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pipette.pick_up_tip()
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pipette.home()
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pipette.drop_tip()
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pipette.pick_up_tip()
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well = source_labware["A3"]
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# directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate
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pipette.move_to(well.bottom(z=2))
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pipette.mix(10, 10)
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pipette.move_to(well.top(z=5))
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pipette.blow_out()
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pipette.prepare_to_aspirate()
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pipette.move_to(well.bottom(z=2))
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pipette.aspirate(10, well.bottom(z=2))
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pipette.dispense(10)
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pipette.drop_tip()
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#############################
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# Change the pipette configuration
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pipette.configure_nozzle_layout(
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style=COLUMN,
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start="A12", # Which nozzle to start with
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tip_racks=[partial_tip_rack],
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)
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#############################
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# Pipette do work
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# consolidate does not work with the COLUMN configuration
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# consolidate only has a single well as a destination
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# transfer the row
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pipette.transfer(volume, source_labware["A5"], destination_labware["A5"])
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# distribute does not work with the COLUMN configuration
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# distribute only has a single well as a source
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pipette.pick_up_tip()
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pipette.touch_tip(source_labware["A6"])
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pipette.drop_tip()
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pipette.pick_up_tip()
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pipette.home()
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pipette.drop_tip()
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pipette.pick_up_tip()
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well = source_labware["A7"]
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# directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate
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pipette.move_to(well.bottom(z=2))
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pipette.mix(10, 10)
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pipette.move_to(well.top(z=5))
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pipette.blow_out()
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pipette.prepare_to_aspirate()
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pipette.move_to(well.bottom(z=2))
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pipette.aspirate(10, well.bottom(z=2))
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pipette.dispense(10)
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pipette.drop_tip()

‎analyses-snapshot-testing/files/protocols/Flex_S_2_20_96_None_PARTIAL_COLUMN_HappyPath.py

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from opentrons.protocol_api import PARTIAL_COLUMN
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metadata = {
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"protocolName": "96Channel PARTIAL_COLUMN Happy Path",
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"description": "96 channel pipette and a PARTIAL_COLUMN partial tip configuration.",
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}
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requirements = {
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"robotType": "Flex",
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"apiLevel": "2.20",
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}
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def run(protocol):
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trash = protocol.load_trash_bin("A3") # must load trash bin
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partial_tip_rack = protocol.load_labware(
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load_name="opentrons_flex_96_tiprack_1000ul",
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label="Partial Tip Rack",
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location="D2",
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)
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pipette = protocol.load_instrument(instrument_name="flex_96channel_1000")
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# Which nozzle to use on the pipette
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# Think of the nozzles from above like you are looking down on a 96-well plate
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# Picking up partial columns with the backmost nozzles is currently not supported. Setting
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# ``style=PARTIAL_COLUMN`` and either ``start="A1"`` or ``start="A12"`` will raise an error.
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# H1 means pipette overhang NE
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# H12 means pipette overhang NW
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pipette.configure_nozzle_layout(
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style=PARTIAL_COLUMN,
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start="H1",
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end="D1", # pick up the first 5
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tip_racks=[partial_tip_rack],
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)
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source_labware = protocol.load_labware(
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load_name="nest_96_wellplate_100ul_pcr_full_skirt",
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label="Liquid Transfer - Source Labware",
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location="C2",
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)
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destination_labware = protocol.load_labware(
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load_name="nest_96_wellplate_100ul_pcr_full_skirt",
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label="Liquid Transfer - Destination Labware",
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location="C3",
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)
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volume = 10 # Default volume for actions that require it
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#############################
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# Pipette do work
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# consolidate does not work with the PARTIAL_COLUMN configuration
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# consolidate only has a single well as a destination
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# transfer the row
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pipette.transfer(volume, source_labware["D1"], destination_labware["D1"])
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# distribute does not work with the PARTIAL_COLUMN configuration
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# distribute only has a single well as a source
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pipette.pick_up_tip()
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pipette.touch_tip(source_labware["D2"])
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pipette.drop_tip()
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pipette.pick_up_tip()
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pipette.home()
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pipette.drop_tip()
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pipette.pick_up_tip()
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well = source_labware["D3"]
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# directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate
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pipette.move_to(well.bottom(z=2))
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pipette.mix(10, 10)
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pipette.move_to(well.top(z=5))
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pipette.blow_out()
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pipette.prepare_to_aspirate()
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pipette.move_to(well.bottom(z=2))
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pipette.aspirate(10, well.bottom(z=2))
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pipette.dispense(10)
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pipette.drop_tip()
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#############################
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# Change the pipette configuration
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pipette.configure_nozzle_layout(
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style=PARTIAL_COLUMN,
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start="H1", # Which nozzle to start with
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end="G1", # 2 - the remaining 2 in the column
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tip_racks=[partial_tip_rack],
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)
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#############################
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# Pipette do work
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# consolidate does not work with the PARTIAL_COLUMN configuration
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# consolidate only has a single well as a destination
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# transfer the row
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pipette.transfer(volume, source_labware["B5"], destination_labware["B5"])
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# distribute does not work with the PARTIAL_COLUMN configuration
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# distribute only has a single well as a source
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pipette.pick_up_tip()
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pipette.touch_tip(source_labware["B6"])
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pipette.drop_tip()
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pipette.pick_up_tip()
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pipette.home()
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pipette.drop_tip()
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pipette.pick_up_tip()
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well = source_labware["B7"]
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# directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate
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pipette.move_to(well.bottom(z=2))
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pipette.mix(10, 10)
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pipette.move_to(well.top(z=5))
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pipette.blow_out()
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pipette.prepare_to_aspirate()
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pipette.move_to(well.bottom(z=2))
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pipette.aspirate(10, well.bottom(z=2))
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pipette.dispense(10)
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pipette.drop_tip()

‎analyses-snapshot-testing/files/protocols/Flex_S_2_20_96_None_ROW_HappyPath.py

Lines changed: 121 additions & 0 deletions
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from opentrons.protocol_api import ROW
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metadata = {
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"protocolName": "96Channel ROW Happy Path",
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"description": "96 channel pipette and a ROW partial tip configuration.",
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}
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requirements = {
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"robotType": "Flex",
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"apiLevel": "2.20",
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}
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def run(protocol):
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trash = protocol.load_trash_bin("A3") # must load trash bin
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partial_tip_rack = protocol.load_labware(
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load_name="opentrons_flex_96_tiprack_1000ul",
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label="Partial Tip Rack",
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location="B1",
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)
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pipette = protocol.load_instrument(instrument_name="flex_96channel_1000")
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# Which nozzle to use on the pipette
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# Think of the nozzles from above like you are looking down on a 96-well plate
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# start="A1" Means the North most nozzle row will pickup from the South most row of the tip rack
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# start="H1" Means the South most nozzle row will pickup from the North most row of the tip rack
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pipette.configure_nozzle_layout(
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style=ROW,
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start="A1",
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tip_racks=[partial_tip_rack],
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)
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source_labware = protocol.load_labware(
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load_name="nest_96_wellplate_100ul_pcr_full_skirt",
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label="Liquid Transfer - Source Labware",
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location="C2",
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)
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destination_labware = protocol.load_labware(
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load_name="nest_96_wellplate_100ul_pcr_full_skirt",
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label="Liquid Transfer - Destination Labware",
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location="C3",
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)
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volume = 10 # Default volume for actions that require it
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#############################
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# Pipette do work
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# consolidate is does not work with the ROW configuration
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# consolidate only has a single well as a destination
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# transfer the row
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pipette.transfer(volume, source_labware["A1"], destination_labware["A1"])
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# distribute does not work with the ROW configuration
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# distribute only has a single well as a source
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pipette.pick_up_tip()
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pipette.touch_tip(source_labware["B1"])
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pipette.drop_tip()
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pipette.pick_up_tip()
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pipette.home()
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pipette.drop_tip()
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pipette.pick_up_tip()
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well = source_labware["D1"]
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# directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate
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pipette.move_to(well.bottom(z=2))
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pipette.mix(10, 10)
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pipette.move_to(well.top(z=5))
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pipette.blow_out()
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pipette.prepare_to_aspirate()
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pipette.move_to(well.bottom(z=2))
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pipette.aspirate(10, well.bottom(z=2))
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pipette.dispense(10)
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pipette.drop_tip()
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#############################
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# Change the pipette configuration
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pipette.configure_nozzle_layout(
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style=ROW,
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start="H1", # Which nozzle to start with
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tip_racks=[partial_tip_rack],
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)
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#############################
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# Pipette do work
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# consolidate is does not work with the ROW configuration
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# consolidate only has a single well as a destination
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# transfer the row
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pipette.transfer(volume, source_labware["E1"], destination_labware["E1"])
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# distribute does not work with the ROW configuration
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# distribute only has a single well as a source
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pipette.pick_up_tip()
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pipette.touch_tip(source_labware["F1"])
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pipette.drop_tip()
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pipette.pick_up_tip()
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pipette.home()
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pipette.drop_tip()
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pipette.pick_up_tip()
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well = source_labware["G1"]
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# directly from docs http://sandbox.docs.opentrons.com/edge/v2/new_protocol_api.html#opentrons.protocol_api.InstrumentContext.prepare_to_aspirate
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pipette.move_to(well.bottom(z=2))
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pipette.mix(10, 10)
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pipette.move_to(well.top(z=5))
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pipette.blow_out()
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pipette.prepare_to_aspirate()
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pipette.move_to(well.bottom(z=2))
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pipette.aspirate(10, well.bottom(z=2))
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pipette.dispense(10)
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pipette.drop_tip()

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