You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Copy file name to clipboardexpand all lines: api/docs/v2/basic_commands/liquids.rst
+28-1
Original file line number
Diff line number
Diff line change
@@ -127,13 +127,40 @@ Push Out After Dispense
127
127
128
128
The optional ``push_out`` parameter of ``dispense()`` helps ensure all liquid leaves the tip. Use ``push_out`` for applications that require moving the pipette plunger lower than the default, without performing a full :ref:`blow out <blow-out>`.
129
129
130
-
For example, this dispense action moves the plunger the equivalent of an additional 5 µL beyond where it would stop if ``push_out`` was set to zero or omitted::
130
+
Flex pipettes include a ``push_out`` by default for any dispense that completely empties the attached pipette tip. The default ``push_out`` volume depends on your pipette and tip combination.
131
+
132
+
.. list-table::
133
+
:header-rows: 1
134
+
* - **Pipette**
135
+
- **Tip**
136
+
- **``push_out`` Volume (µL)**
137
+
* - 1- and 8-channel (50 µL)
138
+
-
139
+
* 50
140
+
-
141
+
* Default: 2
142
+
* Low-volume mode: 7
143
+
* - 1-, 8-, and 96-channel (1000 µL)
144
+
-
145
+
* 50
146
+
* 200
147
+
* 1000
148
+
-
149
+
* 7
150
+
* 5
151
+
* 20
152
+
153
+
OT-2 pipettes do not include a ``push_out`` by default.
154
+
155
+
You can change the ``push_out`` volume for any :py:meth:`~.InstrumentContext.dispense` command. For example, this dispense action moves the ``flex_1channel_1000`` pipette plunger the equivalent of an additional 5 µL beyond where it would stop when dispensing 100 µL of liquid.
131
156
132
157
pipette.pick_up_tip()
133
158
pipette.aspirate(100, plate["A1"])
134
159
pipette.dispense(100, plate["B1"], push_out=5)
135
160
pipette.drop_tip()
136
161
162
+
To disable ``push_out`` during a dispense action, set ``push_out=0``.
0 commit comments