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Release version 26.1.99 for --secondary
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README.md

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@@ -21,7 +21,7 @@ Please refer to our [official pbbioconda page](https://github.com/PacificBioscie
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for information on Installation, Support, License, Copyright, and Disclaimer.
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## Latest Version
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Version **26.1.0**: [Full changelog here](#full-changelog)
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Version **26.1.99**: [Full changelog here](#full-changelog)
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## Usage
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_pbmm2_ offers following tools
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- long cigars for tag `CG` with `-L`
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- `X/=` cigars instead of `M` with `--eqx`
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- no overlapping query intervals with [repeated matches trimming](README.md#what-is-repeated-matches-trimming)
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- no secondary alignments are produced with `--secondary=no`
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- no secondary alignments are produced per default (overridable with `--secondary`)
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### What sequence identity filters does _pbmm2_ offer?
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The idea of removing spurious or low-quality alignments is straightforward,
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Per default, unmapped reads are omitted. You can add them to the output BAM file
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with `--unmapped`.
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### Can I output secondary alignments?
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Use `--secondary` to enable secondary alignment output. Secondary alignments
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are independent alternate mappings that skip repeated matches trimming and
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do not participate in SA tag generation.
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Use `--max-secondary-alns N` to retain at most N secondary alignments prior
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to filtering (default: 5). This option is only effective with `--secondary`.
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### Can I output at maximum the N best alignments per read?
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Use `-N, --best-n`. If set to `0`, default, maximum filtering is disabled.
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## Full Changelog
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* 26.1.99
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* Add `--secondary`
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* 26.1.0
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* Update `ISOSEQ` preset parameters
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* Add `AS` Alignment score tag

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