@@ -21,7 +21,7 @@ Please refer to our [official pbbioconda page](https://github.com/PacificBioscie
2121for information on Installation, Support, License, Copyright, and Disclaimer.
2222
2323## Latest Version
24- Version ** 26.1.0 ** : [ Full changelog here] ( #full-changelog )
24+ Version ** 26.1.99 ** : [ Full changelog here] ( #full-changelog )
2525
2626## Usage
2727_ pbmm2_ offers following tools
@@ -210,7 +210,7 @@ minimap2 parameters:
210210 - long cigars for tag ` CG ` with ` -L `
211211 - ` X/= ` cigars instead of ` M ` with ` --eqx `
212212 - no overlapping query intervals with [ repeated matches trimming] ( README.md#what-is-repeated-matches-trimming )
213- - no secondary alignments are produced with ` --secondary=no `
213+ - no secondary alignments are produced per default (overridable with ` --secondary ` )
214214
215215### What sequence identity filters does _ pbmm2_ offer?
216216The idea of removing spurious or low-quality alignments is straightforward,
@@ -397,6 +397,13 @@ Yes, `--strip` removes following extraneous tags if the input is BAM,
397397Per default, unmapped reads are omitted. You can add them to the output BAM file
398398with ` --unmapped ` .
399399
400+ ### Can I output secondary alignments?
401+ Use ` --secondary ` to enable secondary alignment output. Secondary alignments
402+ are independent alternate mappings that skip repeated matches trimming and
403+ do not participate in SA tag generation.
404+ Use ` --max-secondary-alns N ` to retain at most N secondary alignments prior
405+ to filtering (default: 5). This option is only effective with ` --secondary ` .
406+
400407### Can I output at maximum the N best alignments per read?
401408Use ` -N, --best-n ` . If set to ` 0 ` , default, maximum filtering is disabled.
402409
@@ -420,6 +427,9 @@ and in the same orientation, which are the only fields that `samtools sort` uses
420427
421428## Full Changelog
422429
430+ * 26.1.99
431+ * Add ` --secondary `
432+
423433* 26.1.0
424434 * Update ` ISOSEQ ` preset parameters
425435 * Add ` AS ` Alignment score tag
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