Hello,
I'm wondering if anyone can assist with some issues I'm having re: locus assignment.
Some of my forward primers include ambiguous nucleotides (K, W, R, etc.) and some of the expected STR motifs are combinations of nucleotide strings (e.g. AC + TG). I have not been able to get CHIIMP to successfully assign sequences to any locus that features either or both of these characteristics - are these not features that CHIIMP can parse, or am I maybe just passing the information in the incorrect format in my locus attribute file?

I appreciate any assistance!