Merge pull request #31 from ressy/release-0.0.9

ressy · web-flow · commit 9fab52a46b70 · 2021-07-20T10:47:17.000-04:00
Release 0.0.9
diff --git a/CHANGELOG.md b/CHANGELOG.md
@@ -1,5 +1,25 @@
 # Changelog
 
+## 0.0.9 - 2021-07-20
+
+### Added
+
+ * `--outdir` argument to set output directory other than current working
+   directory ([#24])
+ * `--no-collapse` argument (and updates to `request` function) to disable
+   automatic combining of results across batched submissions ([#25])
+
+### Fixed
+
+ * Empty config files now result in the usual error message about required
+   options ([#30])
+ * All command-line options now match V-QUEST option names ([#28])
+
+[#30]: https://github.com/ressy/vquest/pull/30
+[#28]: https://github.com/ressy/vquest/pull/28
+[#25]: https://github.com/ressy/vquest/pull/25
+[#24]: https://github.com/ressy/vquest/pull/24
+
 ## 0.0.8 - 2021-07-13
 
 ### Fixed
@@ -33,7 +53,7 @@
 
 ### Added
 
- * `--align` argument (via `airr_to_fasta` function) for exraction of sequence
+ * `--align` argument (via `airr_to_fasta` function) for extraction of sequence
    alignment FASTA from AIRR results ([#1])
  * Error messages sent by the server are now raised as an exception containing
    the server-provided message(s) ([#7])
diff --git a/README.md b/README.md
@@ -4,7 +4,7 @@
 
 [IMGT](http://imgt.org)'s [V-QUEST](http://www.imgt.org/IMGT_vquest/analysis)
 is only available via a web interface.  This Python package automates V-QUEST
-usage by submitting request data like the web form does.  Curently only the
+usage by submitting request data like the web form does.  Currently only the
 "Download AIRR formatted results" option is supported.
 
 Example command-line usage, with rhesus sequences in seqs.fasta:
@@ -13,7 +13,7 @@ Example command-line usage, with rhesus sequences in seqs.fasta:
     vquest --species rhesus-monkey --receptorOrLocusType IG --fileSequences seqs.fasta
 
 The output is saved to `Parameters.txt` and `vquest_airr.tsv` (the files
-V-QUEST provides in a zip archive) in the working directory.
+V-QUEST provides in a zip archive) in the working directory by default.
 
 Or with `--align` to automatically extract the alignment as FASTA:
 
@@ -33,14 +33,14 @@ Here the output is a dictionary of filenames to contents.
 
 The only required options are species, receptorOrLocusType, and either
 fileSequences or sequences (to provide sequences directly as text).  Options
-can be given via command-line arguemnts or one or more YAML configuration
+can be given via command-line arguments or one or more YAML configuration
 files.  See [data/defaults.yml](data/defaults.yml) and `./vquest.py --help` for
 details.
 
 The web form will only accept 50 sequences at a time, so the sequences given
-here are grouped into chunks of 50, submitted, and the results combined.  A
-delay (default 1 second) is used between submissions to avoid being impolite to
-the server.
+here are grouped into chunks of 50, submitted, and (by default) the results
+automatically combined.  A delay (default 1 second) is used between submissions
+to avoid being impolite to the server.
 
  * V-QUEST: <http://www.imgt.org/IMGT_vquest/analysis>
  * V-QUEST docs: <http://www.imgt.org/IMGT_vquest/user_guide#intro>
diff --git a/test_vquest/data/test_vquest/TestVquestCustom/config.yml b/test_vquest/data/test_vquest/TestVquestCustom/config.yml
@@ -1,6 +1,6 @@
 species: rhesus-monkey
 receptorOrLocusType: IG
-v_regionsearchindel: true
+V_REGIONsearchIndel: true
 sequences: |
   >IGKV2-ACR*02
   GACATTGTGATGACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCAGCCTCCATCTCCTGCAGGTCTAGTCA
diff --git a/test_vquest/data/test_vquest/TestVquestCustom/config_inline.yml b/test_vquest/data/test_vquest/TestVquestCustom/config_inline.yml
@@ -0,0 +1,11 @@
+species: rhesus-monkey
+receptorOrLocusType: IG
+V_REGIONsearchIndel: true
+resultType: excel
+xv_outputtype: 3
+sequences: |
+  >IGKV2-ACR*02
+  GACATTGTGATGACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCAGCCTCCATCTCCTGCAGGTCTAGTCA
+  GAGCCTCTTGGATAGTGACGGGTACACCTGTTTGGACTGGTACCTGCAGAAGCCAGGCCAGTCTCCACAGCTCCTGATCT
+  ATGAGGTTTCCAACCGGGTCTCTGGAGTCCCTGACAGGTTCAGTGGCAGTGGGTCAGNCACTGATTTCACACTGAAAATC
+  AGCCGGGTGGAAGCTGAGGATGTTGGGGTGTATTACTGTATGCAAAGTATAGAGTTTCCTCC
diff --git a/test_vquest/data/test_vquest/TestVquestCustom/expected/Parameters.txt b/test_vquest/data/test_vquest/TestVquestCustom/expected/Parameters.txt
@@ -0,0 +1,12 @@
+Date	Wed Dec 02 19:18:14 CET 2020	
+IMGT/V-QUEST program version	3.5.21	
+IMGT/V-QUEST reference directory release	202049-2	
+Species	Macaca mulatta	
+Receptor type or locus	IG	
+IMGT/V-QUEST reference directory set	F+ORF+ in-frame P	
+Search for insertions and deletions	yes	
+Nb of nucleotides to add (or exclude) in 3' of the V-REGION for the evaluation of the alignment score	0	
+Nb of nucleotides to exclude in 5' of the V-REGION for the evaluation of the nb of mutations	0	
+Analysis of scFv	no	
+Number of submitted sequences	1	
+
diff --git a/test_vquest/data/test_vquest/TestVquestCustom/expected/vquest_airr.tsv b/test_vquest/data/test_vquest/TestVquestCustom/expected/vquest_airr.tsv
@@ -0,0 +1,2 @@
+sequence_id	sequence	sequence_aa	rev_comp	productive	complete_vdj	vj_in_frame	stop_codon	locus	v_call	d_call	j_call	c_call	sequence_alignment	sequence_alignment_aa	germline_alignment	germline_alignment_aa	junction	junction_aa	np1	np1_aa	np2	np2_aa	cdr1	cdr1_aa	cdr2	cdr2_aa	cdr3	cdr3_aa	fwr1	fwr1_aa	fwr2	fwr2_aa	fwr3	fwr3_aa	fwr4	fwr4_aa	v_score	v_identity	v_support	v_cigar	d_score	d_identity	d_support	d_cigar	j_score	j_identity	j_support	j_cigar	c_score	c_identity	c_support	c_cigar	v_sequence_start	v_sequence_end	v_germline_start	v_germline_end	v_alignment_start	v_alignment_end	d_sequence_start	d_sequence_end	d_germline_start	d_germline_end	d_alignment_start	d_alignment_end	j_sequence_start	j_sequence_end	j_germline_start	j_germline_end	j_alignment_start	j_alignment_end	cdr1_start	cdr1_end	cdr2_start	cdr2_end	cdr3_start	cdr3_end	fwr1_start	fwr1_end	fwr2_start	fwr2_end	fwr3_start	fwr3_end	fwr4_start	fwr4_end	v_sequence_alignment	v_sequence_alignment_aa	d_sequence_alignment	d_sequence_alignment_aa	j_sequence_alignment	j_sequence_alignment_aa	c_sequence_alignment	c_sequence_alignment_aa	v_germline_alignment	v_germline_alignment_aa	d_germline_alignment	d_germline_alignment_aa	j_germline_alignment	j_germline_alignment_aa	c_germline_alignment	c_germline_alignment_aa	junction_length	junction_aa_length	np1_length	np2_length	n1_length	n2_length	p3v_length	p5d_length	p3d_length	p5j_length	consensus_count	duplicate_count	cell_id	clone_id	rearrangement_id	repertoire_id	rearrangement_set_id	sequence_analysis_category	d_number	5prime_trimmed_n_nb	3prime_trimmed_n_nb	insertions	deletions	junction_decryption
+IGKV2-ACR*02	gacattgtgatgacccagactccactctccctgcccgtcacccctggagagccagcctccatctcctgcaggtctagtcagagcctcttggatagtgacgggtacacctgtttggactggtacctgcagaagccaggccagtctccacagctcctgatctatgaggtttccaaccgggtctctggagtccctgacaggttcagtggcagtgggtcagncactgatttcacactgaaaatcagccgggtggaagctgaggatgttggggtgtattactgtatgcaaagtatagagtttcctcc		F	F				IGK	Macmul IGKV2S20*01 F				gacattgtgatgacccagactccactctccctgcccgtcacccctggagagccagcctccatctcctgcaggtctagtcagagcctcttggatagt...gacgggtacacctgtttggactggtacctgcagaagccaggccagtctccacagctcctgatctatgaggtt.....................tccaaccgggtctctggagtccct...gacaggttcagtggcagtggg......tcagncactgatttcacactgaaaatcagccgggtggaagctgaggatgttggggtgtattactgtatgcaaagtatagagtttcctcc	DIVMTQTPLSLPVTPGEPASISCRSSQSLLDS.DGYTCLDWYLQKPGQSPQLLIYEV.......SNRVSGVP.DRFSGSG..SXTDFTLKISRVEAEDVGVYYCMQSIEFP	gatattgtgatgacccagactccactctccctgccagtcacccctggagagccggcctccatctcctgcaggtctagtcagagcctcttggatagtgaggatggaaacacctatttggaatggtacctgcagaagccaggccagtctccacagcccttgatttatgaggtt.....................tccaaccgggcctctggagtccca...gacaggttcagtggcagtggg......tcagacactgatttcacactgaaaatcagcagagtggaggctgaggatgttggggtttattactgcatgcaaggtatagagtatcctcc	DIVMTQTPLSLPVTPGEPASISCRSSQSLLDSEDGNTYLEWYLQKPGQSPQPLIYEV.......SNRASGVP.DRFSGSG..SDTDFTLKISRVEAEDVGVYYCMQGIEYP							cagagcctcttggatagtgacgggtacacctgt	QSLLDSDGYTC	gaggtttcc	EVS	atgcaaagtatagagtttcctcc	MQSIEFP	gacattgtgatgacccagactccactctccctgcccgtcacccctggagagccagcctccatctcctgcaggtctagt	DIVMTQTPLSLPVTPGEPASISCRSS	ttggactggtacctgcagaagccaggccagtctccacagctcctgatctat	LDWYLQKPGQSPQLLIY	aaccgggtctctggagtccctgacaggttcagtggcagtgggtcagncactgatttcacactgaaaatcagccgggtggaagctgaggatgttggggtgtattactgt	NRVSGVPDRFSGSGSXTDFTLKISRVEAEDVGVYYC			1294	93.20		2=1X32=1X17=1X42=3D2=1X2=2X6=1X6=1X34=1X1=1X4=1X19=1X12=1X25=1M25=1X1=1X5=1X17=1X8=1X6=1X9=1X6=													1	302	1	335	1	335													79	111	163	171	280	302	1	78	112	162	172	279			gacattgtgatgacccagactccactctccctgcccgtcacccctggagagccagcctccatctcctgcaggtctagtcagagcctcttggatagt...gacgggtacacctgtttggactggtacctgcagaagccaggccagtctccacagctcctgatctatgaggtt.....................tccaaccgggtctctggagtccct...gacaggttcagtggcagtggg......tcagncactgatttcacactgaaaatcagccgggtggaagctgaggatgttggggtgtattactgtatgcaaagtatagagtttcctcc	DIVMTQTPLSLPVTPGEPASISCRSSQSLLDS.DGYTCLDWYLQKPGQSPQLLIYEV.......SNRVSGVP.DRFSGSG..SXTDFTLKISRVEAEDVGVYYCMQSIEFP							gatattgtgatgacccagactccactctccctgccagtcacccctggagagccggcctccatctcctgcaggtctagtcagagcctcttggatagtgaggatggaaacacctatttggaatggtacctgcagaagccaggccagtctccacagcccttgatttatgaggtt.....................tccaaccgggcctctggagtccca...gacaggttcagtggcagtggg......tcagacactgatttcacactgaaaatcagcagagtggaggctgaggatgttggggtttattactgcatgcaaggtatagagtatcctcc	DIVMTQTPLSLPVTPGEPASISCRSSQSLLDSEDGNTYLEWYLQKPGQSPQPLIYEV.......SNRASGVP.DRFSGSG..SDTDFTLKISRVEAEDVGVYYCMQGIEYP									0		0		0	0	0	0								2 (indelcorr)	0	0	0		in CDR1-IMGT, from codon 33 of V-REGION: 3 nucleotides (from position 97 in the user submitted sequence), (do not cause frameshift)	
diff --git a/test_vquest/data/test_vquest/TestVquestEmpty/config.yml b/test_vquest/data/test_vquest/TestVquestEmpty/config.yml
diff --git a/test_vquest/data/test_vquest/TestVquestInvalid/config.yml b/test_vquest/data/test_vquest/TestVquestInvalid/config.yml
@@ -0,0 +1,8 @@
+species: rhesus-monkey
+receptorOrLocusType: antibody # not valid!
+sequences: |
+  >IGKV2-ACR*02
+  GACATTGTGATGACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCAGCCTCCATCTCCTGCAGGTCTAGTCA
+  GAGCCTCTTGGATAGTGACGGGTACACCTGTTTGGACTGGTACCTGCAGAAGCCAGGCCAGTCTCCACAGCTCCTGATCT
+  ATGAGGTTTCCAACCGGGTCTCTGGAGTCCCTGACAGGTTCAGTGGCAGTGGGTCAGNCACTGATTTCACACTGAAAATC
+  AGCCGGGTGGAAGCTGAGGATGTTGGGGTGTATTACTGTATGCAAAGTATAGAGTTTCCTCC
diff --git a/test_vquest/data/test_vquest/TestVquestInvalid/config_inline.yml b/test_vquest/data/test_vquest/TestVquestInvalid/config_inline.yml
@@ -0,0 +1,10 @@
+species: rhesus-monkey
+receptorOrLocusType: antibody # not valid!
+resultType: excel
+xv_outputtype: 3
+sequences: |
+  >IGKV2-ACR*02
+  GACATTGTGATGACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCAGCCTCCATCTCCTGCAGGTCTAGTCA
+  GAGCCTCTTGGATAGTGACGGGTACACCTGTTTGGACTGGTACCTGCAGAAGCCAGGCCAGTCTCCACAGCTCCTGATCT
+  ATGAGGTTTCCAACCGGGTCTCTGGAGTCCCTGACAGGTTCAGTGGCAGTGGGTCAGNCACTGATTTCACACTGAAAATC
+  AGCCGGGTGGAAGCTGAGGATGTTGGGGTGTATTACTGTATGCAAAGTATAGAGTTTCCTCC
diff --git a/test_vquest/data/test_vquest/TestVquestSimple/config_inline.yml b/test_vquest/data/test_vquest/TestVquestSimple/config_inline.yml
@@ -0,0 +1,10 @@
+species: rhesus-monkey
+receptorOrLocusType: IG
+resultType: excel
+xv_outputtype: 3
+sequences: |
+  >IGKV2-ACR*02
+  GACATTGTGATGACCCAGACTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCAGCCTCCATCTCCTGCAGGTCTAGTCA
+  GAGCCTCTTGGATAGTGACGGGTACACCTGTTTGGACTGGTACCTGCAGAAGCCAGGCCAGTCTCCACAGCTCCTGATCT
+  ATGAGGTTTCCAACCGGGTCTCTGGAGTCCCTGACAGGTTCAGTGGCAGTGGGTCAGNCACTGATTTCACACTGAAAATC
+  AGCCGGGTGGAAGCTGAGGATGTTGGGGTGTATTACTGTATGCAAAGTATAGAGTTTCCTCC
diff --git a/test_vquest/data/test_vquest/TestVquestSimple/expected/Parameters.txt b/test_vquest/data/test_vquest/TestVquestSimple/expected/Parameters.txt
@@ -0,0 +1,12 @@
+Date	Tue Dec 01 22:08:11 CET 2020	
+IMGT/V-QUEST program version	3.5.21	
+IMGT/V-QUEST reference directory release	202049-2	
+Species	Macaca mulatta	
+Receptor type or locus	IG	
+IMGT/V-QUEST reference directory set	F+ORF+ in-frame P	
+Search for insertions and deletions	no	
+Nb of nucleotides to add (or exclude) in 3' of the V-REGION for the evaluation of the alignment score	0	
+Nb of nucleotides to exclude in 5' of the V-REGION for the evaluation of the nb of mutations	0	
+Analysis of scFv	no	
+Number of submitted sequences	1	
+
diff --git a/test_vquest/data/test_vquest/TestVquestSimple/expected/vquest_airr.tsv b/test_vquest/data/test_vquest/TestVquestSimple/expected/vquest_airr.tsv
@@ -0,0 +1,2 @@
+sequence_id	sequence	sequence_aa	rev_comp	productive	complete_vdj	vj_in_frame	stop_codon	locus	v_call	d_call	j_call	c_call	sequence_alignment	sequence_alignment_aa	germline_alignment	germline_alignment_aa	junction	junction_aa	np1	np1_aa	np2	np2_aa	cdr1	cdr1_aa	cdr2	cdr2_aa	cdr3	cdr3_aa	fwr1	fwr1_aa	fwr2	fwr2_aa	fwr3	fwr3_aa	fwr4	fwr4_aa	v_score	v_identity	v_support	v_cigar	d_score	d_identity	d_support	d_cigar	j_score	j_identity	j_support	j_cigar	c_score	c_identity	c_support	c_cigar	v_sequence_start	v_sequence_end	v_germline_start	v_germline_end	v_alignment_start	v_alignment_end	d_sequence_start	d_sequence_end	d_germline_start	d_germline_end	d_alignment_start	d_alignment_end	j_sequence_start	j_sequence_end	j_germline_start	j_germline_end	j_alignment_start	j_alignment_end	cdr1_start	cdr1_end	cdr2_start	cdr2_end	cdr3_start	cdr3_end	fwr1_start	fwr1_end	fwr2_start	fwr2_end	fwr3_start	fwr3_end	fwr4_start	fwr4_end	v_sequence_alignment	v_sequence_alignment_aa	d_sequence_alignment	d_sequence_alignment_aa	j_sequence_alignment	j_sequence_alignment_aa	c_sequence_alignment	c_sequence_alignment_aa	v_germline_alignment	v_germline_alignment_aa	d_germline_alignment	d_germline_alignment_aa	j_germline_alignment	j_germline_alignment_aa	c_germline_alignment	c_germline_alignment_aa	junction_length	junction_aa_length	np1_length	np2_length	n1_length	n2_length	p3v_length	p5d_length	p3d_length	p5j_length	consensus_count	duplicate_count	cell_id	clone_id	rearrangement_id	repertoire_id	rearrangement_set_id	sequence_analysis_category	d_number	5prime_trimmed_n_nb	3prime_trimmed_n_nb	insertions	deletions	junction_decryption
+IGKV2-ACR*02	gacattgtgatgacccagactccactctccctgcccgtcacccctggagagccagcctccatctcctgcaggtctagtcagagcctcttggatagtgacgggtacacctgtttggactggtacctgcagaagccaggccagtctccacagctcctgatctatgaggtttccaaccgggtctctggagtccctgacaggttcagtggcagtgggtcagncactgatttcacactgaaaatcagccgggtggaagctgaggatgttggggtgtattactgtatgcaaagtatagagtttcctcc		F	F				IGK	Macmul IGKV2S20*01 F				gacattgtgatgacccagactccactctccctgcccgtcacccctggagagccagcctccatctcctgcaggtctagtcagagcctcttggatagt...gacgggtacacctgtttggactggtacctgcagaagccaggccagtctccacagctcctgatctatgaggtt.....................tccaaccgggtctctggagtccct...gacaggttcagtggcagtggg......tcagncactgatttcacactgaaaatcagccgggtggaagctgaggatgttggggtgtattactgtatgcaaagtatagagtttcctcc	DIVMTQTPLSLPVTPGEPASISCRSSQSLLDS.DGYTCLDWYLQKPGQSPQLLIYEV.......SNRVSGVP.DRFSGSG..SXTDFTLKISRVEAEDVGVYYCMQSIEFP	gatattgtgatgacccagactccactctccctgccagtcacccctggagagccggcctccatctcctgcaggtctagtcagagcctcttggatagtgaggatggaaacacctatttggaatggtacctgcagaagccaggccagtctccacagcccttgatttatgaggtt.....................tccaaccgggcctctggagtccca...gacaggttcagtggcagtggg......tcagacactgatttcacactgaaaatcagcagagtggaggctgaggatgttggggtttattactgcatgcaaggtatagagtatcctcc	DIVMTQTPLSLPVTPGEPASISCRSSQSLLDSEDGNTYLEWYLQKPGQSPQPLIYEV.......SNRASGVP.DRFSGSG..SDTDFTLKISRVEAEDVGVYYCMQGIEYP							cagagcctcttggatagtgacgggtacacctgt	QSLLDSDGYTC	gaggtttcc	EVS	atgcaaagtatagagtttcctcc	MQSIEFP	gacattgtgatgacccagactccactctccctgcccgtcacccctggagagccagcctccatctcctgcaggtctagt	DIVMTQTPLSLPVTPGEPASISCRSS	ttggactggtacctgcagaagccaggccagtctccacagctcctgatctat	LDWYLQKPGQSPQLLIY	aaccgggtctctggagtccctgacaggttcagtggcagtgggtcagncactgatttcacactgaaaatcagccgggtggaagctgaggatgttggggtgtattactgt	NRVSGVPDRFSGSGSXTDFTLKISRVEAEDVGVYYC			1294	93.20		2=1X32=1X17=1X42=3D2=1X2=2X6=1X6=1X34=1X1=1X4=1X19=1X12=1X25=1M25=1X1=1X5=1X17=1X8=1X6=1X9=1X6=													1	302	1	335	1	335													79	111	163	171	280	302	1	78	112	162	172	279			gacattgtgatgacccagactccactctccctgcccgtcacccctggagagccagcctccatctcctgcaggtctagtcagagcctcttggatagt...gacgggtacacctgtttggactggtacctgcagaagccaggccagtctccacagctcctgatctatgaggtt.....................tccaaccgggtctctggagtccct...gacaggttcagtggcagtggg......tcagncactgatttcacactgaaaatcagccgggtggaagctgaggatgttggggtgtattactgtatgcaaagtatagagtttcctcc	DIVMTQTPLSLPVTPGEPASISCRSSQSLLDS.DGYTCLDWYLQKPGQSPQLLIYEV.......SNRVSGVP.DRFSGSG..SXTDFTLKISRVEAEDVGVYYCMQSIEFP							gatattgtgatgacccagactccactctccctgccagtcacccctggagagccggcctccatctcctgcaggtctagtcagagcctcttggatagtgaggatggaaacacctatttggaatggtacctgcagaagccaggccagtctccacagcccttgatttatgaggtt.....................tccaaccgggcctctggagtccca...gacaggttcagtggcagtggg......tcagacactgatttcacactgaaaatcagcagagtggaggctgaggatgttggggtttattactgcatgcaaggtatagagtatcctcc	DIVMTQTPLSLPVTPGEPASISCRSSQSLLDSEDGNTYLEWYLQKPGQSPQPLIYEV.......SNRASGVP.DRFSGSG..SDTDFTLKISRVEAEDVGVYYCMQGIEYP									0		0		0	0	0	0								1 (noindelsearch)	0	0	0			
diff --git a/test_vquest/test_util.py b/test_vquest/test_util.py
@@ -24,3 +24,38 @@ def test_chunker(self):
         for chunk in util.chunker(range(5), 5):
             chunks.append(chunk)
         self.assertEqual([[0, 1, 2, 3, 4]], chunks)
+
+class TestUnzip(unittest.TestCase):
+    """Basic test of the unzip helper."""
+
+    def test_unzip(self):
+        """Test that binary ZIP data with an empty file can be extracted."""
+        self.assertEqual(
+            util.unzip(bytes.fromhex(
+                "504b03040a0000000000ab6c"
+                "ef5200000000000000000000"
+                "000008001c00746573742e64"
+                "617455540900035272f06052"
+                "72f06075780b000104e90300"
+                "0004e9030000504b01021e03"
+                "0a0000000000ab6cef520000"
+                "000000000000000000000800"
+                "18000000000000000000b481"
+                "00000000746573742e646174"
+                "55540500035272f06075780b"
+                "000104e903000004e9030000"
+                "504b05060000000001000100"
+                "4e000000420000000000")),
+            {"test.dat": b""})
+
+class TestAirrToFasta(unittest.TestCase):
+    """Basic test of the airr_to_fastas helper."""
+
+    def test_airr_to_fasta(self):
+        """Test that FASTA is generated from AIRR TSV."""
+        expected = ">1\nACTG\n>2\nCGTA\n"
+        observed = util.airr_to_fasta(
+            "sequence_id\tsequence\tsequence_alignment\n"
+            "1\tACTG\tACTG\n"
+            "2\t\tCGTA\n")
+        self.assertEqual(observed, expected)
diff --git a/test_vquest/test_vquest.py b/test_vquest/test_vquest.py
diff --git a/vquest/__main__.py b/vquest/__main__.py
diff --git a/vquest/data/options.yml b/vquest/data/options.yml
diff --git a/vquest/request.py b/vquest/request.py
diff --git a/vquest/version.py b/vquest/version.py

Original file line number	Diff line number	Diff line change
`@@ -0,0 +1,2 @@`
	`1`	+sequence_id sequence sequence_aa rev_comp productive complete_vdj vj_in_frame stop_codon locus v_call d_call j_call c_call sequence_alignment sequence_alignment_aa germline_alignment germline_alignment_aa junction junction_aa np1 np1_aa np2 np2_aa cdr1 cdr1_aa cdr2 cdr2_aa cdr3 cdr3_aa fwr1 fwr1_aa fwr2 fwr2_aa fwr3 fwr3_aa fwr4 fwr4_aa v_score v_identity v_support v_cigar d_score d_identity d_support d_cigar j_score j_identity j_support j_cigar c_score c_identity c_support c_cigar v_sequence_start v_sequence_end v_germline_start v_germline_end v_alignment_start v_alignment_end d_sequence_start d_sequence_end d_germline_start d_germline_end d_alignment_start d_alignment_end j_sequence_start j_sequence_end j_germline_start j_germline_end j_alignment_start j_alignment_end cdr1_start cdr1_end cdr2_start cdr2_end cdr3_start cdr3_end fwr1_start fwr1_end fwr2_start fwr2_end fwr3_start fwr3_end fwr4_start fwr4_end v_sequence_alignment v_sequence_alignment_aa d_sequence_alignment d_sequence_alignment_aa j_sequence_alignment j_sequence_alignment_aa c_sequence_alignment c_sequence_alignment_aa v_germline_alignment v_germline_alignment_aa d_germline_alignment d_germline_alignment_aa j_germline_alignment j_germline_alignment_aa c_germline_alignment c_germline_alignment_aa junction_length junction_aa_length np1_length np2_length n1_length n2_length p3v_length p5d_length p3d_length p5j_length consensus_count duplicate_count cell_id clone_id rearrangement_id repertoire_id rearrangement_set_id sequence_analysis_category d_number 5prime_trimmed_n_nb 3prime_trimmed_n_nb insertions deletions junction_decryption
	`2`	+IGKV2-ACR02 gacattgtgatgacccagactccactctccctgcccgtcacccctggagagccagcctccatctcctgcaggtctagtcagagcctcttggatagtgacgggtacacctgtttggactggtacctgcagaagccaggccagtctccacagctcctgatctatgaggtttccaaccgggtctctggagtccctgacaggttcagtggcagtgggtcagncactgatttcacactgaaaatcagccgggtggaagctgaggatgttggggtgtattactgtatgcaaagtatagagtttcctcc F F IGK Macmul IGKV2S2001 F gacattgtgatgacccagactccactctccctgcccgtcacccctggagagccagcctccatctcctgcaggtctagtcagagcctcttggatagt...gacgggtacacctgtttggactggtacctgcagaagccaggccagtctccacagctcctgatctatgaggtt.....................tccaaccgggtctctggagtccct...gacaggttcagtggcagtggg......tcagncactgatttcacactgaaaatcagccgggtggaagctgaggatgttggggtgtattactgtatgcaaagtatagagtttcctcc DIVMTQTPLSLPVTPGEPASISCRSSQSLLDS.DGYTCLDWYLQKPGQSPQLLIYEV.......SNRVSGVP.DRFSGSG..SXTDFTLKISRVEAEDVGVYYCMQSIEFP gatattgtgatgacccagactccactctccctgccagtcacccctggagagccggcctccatctcctgcaggtctagtcagagcctcttggatagtgaggatggaaacacctatttggaatggtacctgcagaagccaggccagtctccacagcccttgatttatgaggtt.....................tccaaccgggcctctggagtccca...gacaggttcagtggcagtggg......tcagacactgatttcacactgaaaatcagcagagtggaggctgaggatgttggggtttattactgcatgcaaggtatagagtatcctcc DIVMTQTPLSLPVTPGEPASISCRSSQSLLDSEDGNTYLEWYLQKPGQSPQPLIYEV.......SNRASGVP.DRFSGSG..SDTDFTLKISRVEAEDVGVYYCMQGIEYP cagagcctcttggatagtgacgggtacacctgt QSLLDSDGYTC gaggtttcc EVS atgcaaagtatagagtttcctcc MQSIEFP gacattgtgatgacccagactccactctccctgcccgtcacccctggagagccagcctccatctcctgcaggtctagt DIVMTQTPLSLPVTPGEPASISCRSS ttggactggtacctgcagaagccaggccagtctccacagctcctgatctat LDWYLQKPGQSPQLLIY aaccgggtctctggagtccctgacaggttcagtggcagtgggtcagncactgatttcacactgaaaatcagccgggtggaagctgaggatgttggggtgtattactgt NRVSGVPDRFSGSGSXTDFTLKISRVEAEDVGVYYC 1294 93.20 2=1X32=1X17=1X42=3D2=1X2=2X6=1X6=1X34=1X1=1X4=1X19=1X12=1X25=1M25=1X1=1X5=1X17=1X8=1X6=1X9=1X6= 1 302 1 335 1 335 79 111 163 171 280 302 1 78 112 162 172 279 gacattgtgatgacccagactccactctccctgcccgtcacccctggagagccagcctccatctcctgcaggtctagtcagagcctcttggatagt...gacgggtacacctgtttggactggtacctgcagaagccaggccagtctccacagctcctgatctatgaggtt.....................tccaaccgggtctctggagtccct...gacaggttcagtggcagtggg......tcagncactgatttcacactgaaaatcagccgggtggaagctgaggatgttggggtgtattactgtatgcaaagtatagagtttcctcc DIVMTQTPLSLPVTPGEPASISCRSSQSLLDS.DGYTCLDWYLQKPGQSPQLLIYEV.......SNRVSGVP.DRFSGSG..SXTDFTLKISRVEAEDVGVYYCMQSIEFP gatattgtgatgacccagactccactctccctgccagtcacccctggagagccggcctccatctcctgcaggtctagtcagagcctcttggatagtgaggatggaaacacctatttggaatggtacctgcagaagccaggccagtctccacagcccttgatttatgaggtt.....................tccaaccgggcctctggagtccca...gacaggttcagtggcagtggg......tcagacactgatttcacactgaaaatcagcagagtggaggctgaggatgttggggtttattactgcatgcaaggtatagagtatcctcc DIVMTQTPLSLPVTPGEPASISCRSSQSLLDSEDGNTYLEWYLQKPGQSPQPLIYEV.......SNRASGVP.DRFSGSG..SDTDFTLKISRVEAEDVGVYYCMQGIEYP 0 0 0 0 0 0 2 (indelcorr) 0 0 0 in CDR1-IMGT, from codon 33 of V-REGION: 3 nucleotides (from position 97 in the user submitted sequence), (do not cause frameshift)