diff --git a/.changeset/popular-knives-guess.md b/.changeset/popular-knives-guess.md
new file mode 100644
index 00000000000..09612f60a32
--- /dev/null
+++ b/.changeset/popular-knives-guess.md
@@ -0,0 +1,5 @@
+---
+'@shopify/polaris': patch
+---
+
+Update DropZone so it does not error on dragenter events for glb/gltf files
diff --git a/polaris-react/src/components/DropZone/DropZone.tsx b/polaris-react/src/components/DropZone/DropZone.tsx
index 25ed2d3ac32..cc88554da1a 100755
--- a/polaris-react/src/components/DropZone/DropZone.tsx
+++ b/polaris-react/src/components/DropZone/DropZone.tsx
@@ -45,7 +45,14 @@ export interface DropZoneProps {
   labelHidden?: boolean;
   /** ID for file input */
   id?: string;
-  /** Allowed file types */
+  /** Allowed file MIME types. Note that model subtypes such as model/gltf-binary
+   * are not recognized by most browsers. In this case, and for other unsupported
+   * MIME types, you can use file extensions in the accept string instead, for
+   * example '.glb', but note that this will disable file validation on drag events,
+   * since the file name is not known until the file is dropped. It is valid to mix
+   * MIME types and extensions in the accept string.
+   * @example 'image/jpeg,video/*'
+   */
   accept?: string;
   /**
    * Whether is a file or an image
@@ -188,16 +195,25 @@ export const DropZone: React.FunctionComponent<DropZoneProps> & {
   const i18n = useI18n();
 
   const getValidatedFiles = useCallback(
-    (files: File[] | DataTransferItem[]) => {
+    (event: DropZoneEvent) => {
+      const files = getDataTransferFiles(event);
       const acceptedFiles: File[] = [];
       const rejectedFiles: File[] = [];
 
-      Array.from(files as File[]).forEach((file: File) => {
-        !fileAccepted(file, accept) ||
-        (customValidator && !customValidator(file))
-          ? rejectedFiles.push(file)
-          : acceptedFiles.push(file);
-      });
+      // File names and extensions are only known on drop events, so if the
+      // 'accept' prop includes any file extensions, we skip validation on
+      // non-drop events (such as dragenter and dragover) and validate
+      // once the user drops the file and the file name is known.
+      if (event.type !== 'drop' && accept?.includes('.')) {
+        acceptedFiles.push(...(files as File[]));
+      } else {
+        Array.from(files as File[]).forEach((file: File) => {
+          !fileAccepted(file, accept) ||
+          (customValidator && !customValidator(file))
+            ? rejectedFiles.push(file)
+            : acceptedFiles.push(file);
+        });
+      }
 
       if (!allowMultiple) {
         acceptedFiles.splice(1, acceptedFiles.length);
@@ -214,9 +230,7 @@ export const DropZone: React.FunctionComponent<DropZoneProps> & {
       stopEvent(event);
       if (disabled) return;
 
-      const fileList = getDataTransferFiles(event);
-
-      const {files, acceptedFiles, rejectedFiles} = getValidatedFiles(fileList);
+      const {files, acceptedFiles, rejectedFiles} = getValidatedFiles(event);
 
       dragTargets.current = [];
 
@@ -239,15 +253,13 @@ export const DropZone: React.FunctionComponent<DropZoneProps> & {
       stopEvent(event);
       if (disabled) return;
 
-      const fileList = getDataTransferFiles(event);
-
       if (event.target && !dragTargets.current.includes(event.target)) {
         dragTargets.current.push(event.target);
       }
 
       if (dragging) return;
 
-      const {rejectedFiles} = getValidatedFiles(fileList);
+      const {rejectedFiles} = getValidatedFiles(event);
 
       setDragging(true);
       setInternalError(rejectedFiles.length > 0);
diff --git a/polaris-react/src/components/DropZone/tests/DropZone.test.tsx b/polaris-react/src/components/DropZone/tests/DropZone.test.tsx
index 6a25e998107..bc4a4ae93c4 100755
--- a/polaris-react/src/components/DropZone/tests/DropZone.test.tsx
+++ b/polaris-react/src/components/DropZone/tests/DropZone.test.tsx
@@ -90,6 +90,37 @@ describe('<DropZone />', () => {
     expect(spy).toHaveBeenCalledWith(files, acceptedFiles, rejectedFiles);
   });
 
+  it('calls the onDrop callback with files, acceptedFiles, and rejectedFiles when the accept prop is an extension', () => {
+    const dropZone = mountWithApp(<DropZone onDrop={spy} accept=".jpg" />);
+    const testFiles = [{type: 'image/jpeg', name: 'cat.jpg'}];
+    fireEvent({wrapper: dropZone, testFiles});
+    expect(spy).toHaveBeenCalledWith(testFiles, testFiles, []);
+  });
+
+  it('calls the onDrop callback with files, acceptedFiles, and rejectedFiles when the mime type is empty', () => {
+    const dropZone = mountWithApp(<DropZone onDrop={spy} accept=".glb" />);
+    const testFiles = [{type: '', name: 'cat.glb'}];
+    fireEvent({wrapper: dropZone, testFiles});
+    expect(spy).toHaveBeenCalledWith(testFiles, testFiles, []);
+  });
+
+  it('calls the onDrop callback with files, acceptedFiles, and rejectedFiles when the accept prop is a combination of mime types and extensions', () => {
+    const dropZone = mountWithApp(
+      <DropZone onDrop={spy} accept="image/jpeg,.glb" />,
+    );
+    const testFiles = [
+      {type: 'image/jpeg', name: 'cat.jpg'},
+      {type: '', name: 'cat.glb'},
+      {type: 'image/png', name: 'cat.png'},
+    ];
+    fireEvent({wrapper: dropZone, testFiles});
+    expect(spy).toHaveBeenCalledWith(
+      testFiles,
+      [testFiles[0], testFiles[1]],
+      [testFiles[2]],
+    );
+  });
+
   it('calls the onDropAccepted callback with acceptedFiles when it accepts only jpeg', () => {
     const dropZone = mountWithApp(
       <DropZone onDropAccepted={spy} accept="image/jpeg" />,
@@ -413,6 +444,20 @@ describe('<DropZone />', () => {
         children: errorOverlayText,
       });
     });
+
+    it('does not render an error on dragenter event when file extensions are accepted', () => {
+      const dropZone = mountWithApp(
+        <DropZone
+          errorOverlayText={errorOverlayText}
+          accept=".glb"
+          variableHeight
+        />,
+      );
+      fireEvent({wrapper: dropZone, eventType: 'dragenter'});
+      expect(dropZone).not.toContainReactComponent(Text, {
+        children: errorOverlayText,
+      });
+    });
   });
 
   describe('onFileDialogClose', () => {