Full documentation: http://www.microbesonline.org/fasttree/
FastTree infers approximately-maximum-likelihood phylogenetic trees from alignments of nucleotide or protein sequences.
FastTree can handle alignments with up to a million of sequences in a reasonable amount of time and memory.
FastTree accepts alignments in fasta or phylip interleaved formats
FastTree protein_alignment > tree
FastTree < protein_alignment > tree
FastTree -out tree protein_alignment
FastTree -nt nucleotide_alignment > tree
FastTree -nt -gtr < nucleotide_alignment > tree
FastTree < nucleotide_alignment > treeExample command-line input for basic FastTree phylogeny
FastTree -log fasttree_test_log -nt sample.fasta > fasttree_test.nwkFor more information, see http://www.microbesonline.org/fasttree/