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README.md

piranha container

Main tool : piranha

Additional tools installed via micromamba:
List of packages in environment: "/opt/conda/envs/piranha"

  Name                                   Version       Build                         Channel    
──────────────────────────────────────────────────────────────────────────────────────────────────
  Mako                                   1.2.0         pypi_0                        pypi       
  _openmp_mutex                          5.1           52_gnu                        pkgs/main  
  alsa-lib                               1.2.16.1      hb03c661_0                    conda-forge
  appdirs                                1.4.4         pyhd8ed1ab_1                  conda-forge
  argparse-dataclass                     2.0.0         pyhd8ed1ab_1                  conda-forge
  attrs                                  26.1.0        pyhcf101f3_0                  conda-forge
  aws-c-auth                             0.10.1        ha62d5e7_3                    conda-forge
  aws-c-cal                              0.9.13        h2c9d079_1                    conda-forge
  aws-c-common                           0.12.6        hb03c661_0                    conda-forge
  aws-c-compression                      0.3.2         h8b1a151_0                    conda-forge
  aws-c-http                             0.10.13       h4bacb7b_0                    conda-forge
  aws-c-io                               0.26.3        hb18f61d_2                    conda-forge
  aws-c-s3                               0.12.0        h1b28b03_1                    pkgs/main  
  aws-c-sdkutils                         0.2.4         h8b1a151_4                    conda-forge
  aws-checksums                          0.2.10        h8b1a151_0                    conda-forge
  backports.zstd                         1.6.0         py312h90b7ffd_0               conda-forge
  tabix                                  1.11          hdfd78af_0                    bioconda   
  tabixpp                                1.1.2         hbefcdb2_4                    bioconda   
  tabulate                               0.10.0        pyhcf101f3_0                  conda-forge
  tar                                    1.35          h3b78370_0                    conda-forge
  tenacity                               9.1.4         pyhcf101f3_0                  conda-forge
  tensordict                             0.1.2         pyh6074d0b_0                  conda-forge
  throttler                              1.2.2         pyhd8ed1ab_0                  conda-forge
  tk                                     8.6.13        noxft_h366c992_103            conda-forge
  toml                                   0.10.2        pyhcf101f3_3                  conda-forge
  tqdm                                   4.68.3        pyh8f84b5b_0                  conda-forge
  traitlets                              5.15.1        pyhcf101f3_0                  conda-forge
  typing-extensions                      4.15.0        h396c80c_0                    conda-forge
  typing_extensions                      4.15.0        pyhcf101f3_0                  conda-forge
  tzdata                                 2025c         hc9c84f9_1                    conda-forge
  urllib3                                2.7.0         pyhd8ed1ab_0                  conda-forge
  vcflib                                 1.0.15        h3fa9d83_1                    bioconda   
  wget                                   1.25.0        h653f8fd_1                    conda-forge
  wheel                                  0.47.0        pyhd8ed1ab_0                  conda-forge
  wrapt                                  1.17.0        py312h5eee18b_0               pkgs/main  
  wurlitzer                              3.1.1         pyhd8ed1ab_1                  conda-forge
  xorg-libice                            1.1.2         hb9d3cd8_0                    conda-forge
  xorg-libsm                             1.2.6         he73a12e_0                    conda-forge
  xorg-libx11                            1.8.13        he1eb515_0                    conda-forge
  xorg-libxau                            1.0.12        hb03c661_1                    conda-forge
  xorg-libxdmcp                          1.1.5         hb03c661_1                    conda-forge
  xorg-libxext                           1.3.7         hb03c661_0                    conda-forge
  xorg-libxfixes                         6.0.2         hb03c661_0                    conda-forge
  xorg-libxi                             1.8.3         hb03c661_0                    conda-forge
  xorg-libxrandr                         1.5.5         hb03c661_0                    conda-forge
  xorg-libxrender                        0.9.12        hb9d3cd8_0                    conda-forge
  xorg-libxt                             1.3.1         hb9d3cd8_0                    conda-forge
  xorg-libxtst                           1.2.5         hb9d3cd8_3                    conda-forge
  yaml                                   0.2.5         h280c20c_3                    conda-forge
  yte                                    1.9.4         pyhd8ed1ab_0                  conda-forge
  zlib                                   1.3.2         h25fd6f3_2                    conda-forge
  zstd                                   1.5.7         hb78ec9c_6                    conda-forge

Full documentation: https://github.com/polio-nanopore/piranha

Poliovirus Investigation Resource Automating Nanopore Haplotype Analysis.

piranha is a tool in development as part of the Poliovirus Sequencing Consortium. It runs an automated analysis pipeline for sequencing VP1 regions of the poliovirus or non-polio enterovirus genome (whole genome analysis to be implemented soon) and produces an interactive report alongside the consensus data.

Example Usage

# run Piranha on a directory of FASTQs (Guppy output)
piranha -i /piranha/test/pak_run/demultiplexed --verbose -b piranha/test/pak_run/barcodes01.csv -t 2 2>&1 | tee piranha.log