Missing tutorial

[HeeeyNathan]

Dear Dr Macher,

I am a novice in the field of metabarcoding analyses, though I am actively working to improve. I came across the TaxonTableTools platform you created and I would like to begin using it to analyse my metabarcoding data in a similar fashion to your paper: "Fit for purpose? Evaluating benthic invertebrate DNA metabarcoding for ecological status class assessment in streams under the Water Framework Directive"

I have installed the platform and can launch it in my browser. However, I am struggling to understand how it works. I have watched your tutorial here for the first iteration of TaxonTableTools and I have downloaded the tutorial files, but when attempting to upload these files, it continuously tells me "Please check your TaXon table. It seems to be empty!".

I am using TaxonTableTools2 and I have not found an online tutorial to help me understand the workflow. Do you by chance have example files and/or an online tutorial link which I can follow?

Any help will be greatly appreciated.

Kind regards,
Dr Nathan Jay Baker

[OndroV]

Hi Nathan,

I attach a TaXon table in a TTT2-compatible format, made from the old tutorial files.

TTT2_TaXon_table_oldtutorial_20260416.xlsx

*attachment updated on 20260416 because TTT2 now requires the ID column to be named "Hash", and column Kingdom is now required for the functionality "Simplify Taxon Table".

Currently, almost all the modules throw warnings or stop with errors, but it's possible to copy our own data into the compatible TaXon table and get at least some nice stats and plots. For some features I can still use the old TTT 1.4.8, but after some software updates it started crashing in certain modules and I guess there would be issues installing it now.

I'm looking forward to a stable TTT2 as well. Fingers crossed, Till!
Best,
Ondrej

[HeeeyNathan]

Hi Ondrej,

Thanks a bunch for the updated file. Actually, I finally got it to work after I changed the column name in the read table to "unique_ID" and the column name in the taxonomy table to "unique ID", with the only difference being an underscore vs a space. Nonetheless, I appreciate the updated file you sent and will attempt to run everything again. I managed to get the first version of TTT work on my PC (after a ton of finicking) but then it crashed everytime I did anything slightly more laborious.

Thanks again!

Best,
Nathan