Chromium Next GEM Single Cell ATAC Reagent Kits v1.1, 10X genomics, users guide.
This step synthesizes top single strands.
Notes: scATAC-seq sequencing constructs don't have UMI sequence because it's a DNA sequencing methods, not like RNA sequencing that contain many RNA transcripts to differentiate.
P7 and a sample index are added during library construction via PCR. The final libraries contain the P5 and P7 sequences used in Illumina® bridge amplification.
