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v1.24 (2025.12)
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0Install.sh

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wget -c http://ftp.ensemblgenomes.org/pub/plants/release-51/fasta/arabidopsis_thaliana/dna/Arabidopsis_thaliana.TAIR10.dna.toplevel.fa.gz
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mv Arabidopsis_thaliana.TAIR10.dna.toplevel.fa.gz tair10.fa.gz
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# unzip and index, 3 minutes (解压,建索引,120M 3分钟)
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gunzip tair10.fa.gz`
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gunzip tair10.fa.gz
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time bowtie2-build -f tair10.fa tair10 --threads 4
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cd ${db}
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tar xvzf uniref100.KO.1.dmnd.tar.gz
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#指定数据库
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export CHECKM2DB="/data/meta/db/checkm2/CheckM2_database/uniref100.KO.1.dmnd"
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export CHECKM2DB="${db}/checkm2/CheckM2_database/uniref100.KO.1.dmnd"
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# 测试
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checkm2 testrun
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2StatPlot.sh

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# Cititon(引文): Bai, et al. 2025. EasyMetagenome: A User‐Friendly and Flexible Pipeline for Shotgun Metagenomic Analysis in Microbiome Research. iMeta 4: e70001. https://doi.org/10.1002/imt2.70001
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# Set work directory(设置工作目录)
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wd=/d/meta/result
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wd=/d/Download/github/EasyMetagenome/result
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sd=/d/Download/github/EasyMicrobiome/script
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PATH=$PATH:$sd/../win:$sd
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cd $wd

result/metaphlan4/Cancer_sparcc.txt

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result/metaphlan4/Class.txt

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result/metaphlan4/Family.txt

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