I only got a few regulons after running ctx.

[antss8]

I created a .feather file for non-model species. The .tbl file contains over 2000 TFs. In the end, after running the pyscenic ctx process, I only got 144 regulons.

However, these regulons did not contain any cell-specific TF-targets that have been reported, but their co-expression information could be found in the GRN results.

Is this due to an issue during the construction of the.feather file, or is it because my promoter sequence file did not cover the control area (I only selected 2000bp upstream of the promoter as the control area)?

[antss8]

Among them, there were duplicates in the TFs. After deleting the duplicates, only 73 remained.