NTR: phospholactate phosphatase

[dragon-ai-agent]

Shadow ticket of geneontology/go-ontology#31119

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Please provide as much information as you can:

• Suggested term label:
  phospholactate phosphatase

• Definition (free text)
  Catalysis of the reaction: (2S)-2-phospholactate + H2O = (S)-lactate + phosphate

• Reference, in format PMID:#######
  PMID:27294321

• Gene product name and ID to be annotated to this term
  mouse Pgp (UniProt Q8CHP8)
  yeast PHO13 (UniProt P19881)

• Parent term(s)
  GO:0016791 (phosphatase activity)

• Children terms (if applicable) Should any existing terms that should be moved underneath this new proposed term?

• Synonyms (please specify, EXACT, BROAD, NARROW or RELATED)

• Cross-references
  Rhea:86559 (not yet public)

• Any other information

[cmungall]

@dragon-ai-agent please analyze and implement

[dragon-ai-agent]

🤖 Working on it...

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[dragon-ai-agent]

Implementation Complete

I've analyzed the request and created a new GO term for phospholactate phosphatase activity.

New Term Created: GO:7770035

Property	Value
Name	2-phospholactate phosphatase activity
Definition	Catalysis of the reaction: (2S)-2-phospholactate + H2O = (S)-lactate + phosphate.
Parent	GO:0016791 (phosphatase activity)
References	PMID:27294321, RHEA:86559
Synonyms	phospholactate phosphatase activity (EXACT), (2S)-2-phospholactate phosphohydrolase activity (RELATED)

Analysis Summary

Based on the research of PMID:27294321:

• The enzyme catalyzing this reaction is phosphoglycolate phosphatase (PGP), which has broad substrate specificity
• PGP acts as a "metabolite repair enzyme" that removes toxic glycolytic side products including 2-phospho-L-lactate
• The gene products mentioned (mouse Pgp/UniProt Q8CHP8 and yeast PHO13/UniProt P19881) can be annotated to this new term

Pull Request

PR #21 created with this implementation.

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🤖 Generated by @dragon-ai-agent

• Model: claude-opus-4-5-20251101
• Agent harness: claude-code
• Triggered by: @cmungall
• Run: View workflow run

[cmungall]

@dragon-ai-agent please do this again. I closed the first PR as it was accidentally using old agent instructions

[dragon-ai-agent]

🤖 Working on it...

Follow along: View workflow run

— @dragon-ai-agent

[dragon-ai-agent]

Implementation Complete

I've re-implemented the phospholactate phosphatase activity term with the updated agent instructions.

New Term Created: GO:7770035

Property	Value
Name	phospholactate phosphatase activity
Definition	Catalysis of the reaction: (2S)-2-phospholactate + H2O = (S)-lactate + phosphate.
Parent	GO:0016791 (phosphatase activity)
Reference	PMID:27294321
Synonyms	2-phospholactate phosphatase activity (EXACT), (2S)-2-phospholactate phosphohydrolase activity (RELATED)

Research Summary

Based on PMID:27294321 ("A conserved phosphatase destroys toxic glycolytic side products in mammals and yeast"):

• 2-phospho-L-lactate is produced as a side product of pyruvate kinase
• It inhibits the enzyme producing fructose 2,6-bisphosphate, blocking glycolysis
• Phosphoglycolate phosphatase (PGP) removes this toxic metabolite
• This metabolite repair function is conserved in mammals and yeast

The gene products mentioned (mouse Pgp/UniProt Q8CHP8 and yeast PHO13/UniProt P19881) can be annotated to this new term.

Notes

• RHEA:86559 could not be validated (you noted it was "not yet public"), so it was omitted from the xrefs
• All validation checks pass (make travis_build succeeds)

Pull Request

PR #25: #25

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🤖 Generated by @dragon-ai-agent

• Model: claude-opus-4-5-20251101
• Agent harness: claude-code
• Triggered by: @cmungall
• Run: View workflow run