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EM_ampliseq_analysis

Analysis scripts for EM ampliseq manuscript

Contacts

Alexander Predeus, 2016-2018, predeus at gmail dot com

Publication

A. V. Predeus, E. S. Vashukova, A. S. Glotov, M. M. Danilova, N. S. Osinovskaya, O. V. Malysheva, N. Yu. Shved, N. Ganbarli, M. I. Yarmolinskaya, T. E. Ivashchenko, V. S. Baranov, Next-Generation Sequencing of Matched Ectopic and Eutopic Endometrium Identifies Novel Endometriosis-Related Genes, Russian Journal of Genetics, 2018, 54(11), 1358–1365.

Processing of raw reads

Raw reads generated by AmpliSeq/IonTorrent program are available via GEO database GSE99949.

Reads were processed using rnaquant pipeline. Resulting matrix of gene expression (raw read counts) was analyzed in R using DESeq2 and fgsea.