small Genome size

[jhcaddisfly]

Hi,
I was using MGSE to estimate genome size as follows:

python MGSE.py --bam taxon.sort.bam (Illumina reads mapped to genome) --out test --busco /run_busco/full_table_busco.tsv

But this revealed a very small genome size (half the size of other approaches such as Flow Cytometry and GenomeScope). I also tried python MGSE.py --bam taxon.sort.bam (Illumina reads mapped to genome) --out test --all but the genome size was even smaller.

Do you have any explanation for this observation?

Best,

Jacqueline