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lines changed Original file line number Diff line number Diff line change @@ -15,7 +15,7 @@ task trim_rmdup_subsamp {
1515 Int cpu = 16
1616 Int disk_size_gb = 100
1717
18- String docker = "quay.io/broadinstitute/viral-assemble :2.5.21.0 "
18+ String docker = "quay.io/broadinstitute/viral-core :2.5.21"
1919 }
2020
2121 parameter_meta {
@@ -36,17 +36,14 @@ task trim_rmdup_subsamp {
3636 command <<<
3737 set -ex o pipefail
3838 assembly.py --version | tee VERSION
39- #BAM ->FASTQ-> OutBam? https://github.com/broadinstitute/viral-assemble:2.5.21.0
4039 assembly.py trim_rmdup_subsamp \
41- "~{inBam}" \
42- "~{clipDb}" \
43- "$(pwd)/ outbam.bam" \
44- ~{'--n_reads=' + n_reads }
40+ "~{inBam}" \
41+ "~{clipDb}" \
42+ outbam.bam \
43+ ~{'--n_reads=' + n_reads }
4544
46-
47- #samtools [OutBam -> FASTA]
4845 #-f 4 (f = include only) (4 = unmapped reads) https://broadinstitute.github.io/picard/explain-flags.html
49- samtools fasta "$(pwd)/ outbam.bam" > "~{bam_basename}.fasta"
46+ samtools fasta outbam.bam > "~{bam_basename}.fasta"
5047 >>>
5148
5249 output {
@@ -58,7 +55,6 @@ task trim_rmdup_subsamp {
5855 memory : machine_mem_gb + "GB"
5956 cpu : cpu
6057 disks : "local-disk " + disk_size_gb + " LOCAL"
61-
6258 dx_instance_type : "n2-highmem-4"
6359 }
6460}
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