| pmid | 10415337 | |||
|---|---|---|---|---|
| title | Identification of a novel gene encoding a p53-associated protein. | |||
| authors |
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| journal | Gene | |||
| year | 1999 | |||
| full_text_available | false | |||
| doi | 10.1016/s0378-1119(99)00203-6 |
Authors: Zhou R, Wen H, Ao SZ Journal: Gene (1999) DOI: 10.1016/s0378-1119(99)00203-6
- Gene. 1999 Jul 22;235(1-2):93-101. doi: 10.1016/s0378-1119(99)00203-6.
Identification of a novel gene encoding a p53-associated protein.
Zhou R(1), Wen H, Ao SZ.
Author information: (1)State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, Chinese Academy of Science, Shanghai 200031, People's Republic of China.
p53 exerts important physiological functions in cell-cycle control, gene regulation, cell differentiation, apoptosis and tumor suppression by interacting with many cellular proteins. Using the yeast two-hybrid system, we screened a HeLa cDNA library and identified a novel gene encoding a p53-binding protein (p53BP3). The full-length cDNA of p53BP3 was isolated from a HeLalambdagt10 cDNA library. This predicted protein was composed of 815 amino acids. Sequence analysis indicated that p53BP3 contained two bipartite nuclear localization signals and was confirmed to be a nuclear protein. FISH mapping results showed that this novel gene was located at human chromosome 12, region p11.2-p12.1. Northern blot analysis suggested that p53BP3 was broadly expressed in human tissues. A further study showed that p53BP3 had a homologue in mouse.
DOI: 10.1016/s0378-1119(99)00203-6 PMID: 10415337 [Indexed for MEDLINE]