| pmid | 10873669 | |||||||
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| title | Identification of a novel beta-catenin-interacting protein. | |||||||
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| journal | Biochem Biophys Res Commun | |||||||
| year | 2000 | |||||||
| full_text_available | false | |||||||
| doi | 10.1006/bbrc.2000.3002 |
Authors: Kawajiri A, Itoh N, Fukata M, Nakagawa M, Yamaga M, Iwamatsu A, Kaibuchi K Journal: Biochem Biophys Res Commun (2000) DOI: 10.1006/bbrc.2000.3002
- Biochem Biophys Res Commun. 2000 Jul 5;273(2):712-7. doi: 10.1006/bbrc.2000.3002.
Identification of a novel beta-catenin-interacting protein.
Kawajiri A(1), Itoh N, Fukata M, Nakagawa M, Yamaga M, Iwamatsu A, Kaibuchi K.
Author information: (1)Division of Signal Transduction, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, 630-0101, Japan.
Cadherin is a well-known cell-cell adhesion molecule, and it binds to beta-catenin, which in turn binds to alpha-catenin. However, little is known about the regulatory mechanism underlying the cadherin-mediated cell-cell adhesion. Here we purified two novel beta-catenin-interacting proteins with molecular masses of 180 kDa (p180) and 150 kDa (p150) from bovine brain cytosol by using glutathione S-transferase (GST)-beta-catenin affinity column chromatography. Mass spectral analysis revealed p180 to be identical to KIAA0313 which has a putative Rap1 guanine nucleotide exchange factor (GEF) domain and p150 to be the same as KIAA0705 which has a high degree of sequence similarity to the synaptic scaffolding molecule (S-SCAM), which binds beta-catenin and KIAA0313 in the yeast two-hybrid system and overlay assay, respectively (Ide et al., Biochem. Biophys. Res. Commun. 256, 456-461, 1999; Ohtsuka et al., Biochem. Biophys. Res. Commun. 265, 38-44, 1999). beta-Catenin was coimmunoprecipitated with KIAA0313 in Madin-Darby canine kidney II (MDCKII) cells, bovine brain cytosol, and EL cells. KIAA0313 and beta-catenin were partly colocalized at sites of cell-cell contact in MDCKII cells. Taken together, our data suggest that KIAA0313 associates with beta-catenin through KIAA0705 in vivo at sites of cell-cell contact.
Copyright 2000 Academic Press.
DOI: 10.1006/bbrc.2000.3002 PMID: 10873669 [Indexed for MEDLINE]