diff --git a/biahub/flat_field_correction.py b/biahub/flat_field_correction.py index 0695e3dc..996aa44f 100644 --- a/biahub/flat_field_correction.py +++ b/biahub/flat_field_correction.py @@ -247,7 +247,7 @@ def flat_field_correction_cli( monitor: bool = False, init_only: bool = False, ): - r"""Apply flat field correction across T and selected C axes. + """Apply flat field correction across T and selected C axes. \b SLURM fan-out of positions across a whole plate: @@ -260,7 +260,7 @@ def flat_field_correction_cli( \b In-process run of a single position (e.g. from a Nextflow worker): >>> biahub flat-field --cluster debug -i ./input.zarr/A/1/0 -c ./flat_field_params.yml -o ./output.zarr - """ + """ # noqa: D301 flat_field( input_position_dirpaths=input_position_dirpaths, config_filepath=config_filepath, diff --git a/biahub/virtual_stain.py b/biahub/virtual_stain.py index bba2fd4a..8210805e 100644 --- a/biahub/virtual_stain.py +++ b/biahub/virtual_stain.py @@ -1,441 +1,449 @@ -import os -import shutil -import subprocess +import time from pathlib import Path +from typing import Literal import click import numpy as np import submitit +import torch from iohub.ngff import open_ome_zarr from iohub.ngff.utils import create_empty_plate +from biahub.cli import utils from biahub.cli.monitor import monitor_jobs from biahub.cli.parsing import ( + cluster, + config_filepath, + init_only, input_position_dirpaths, - local, monitor, - num_processes, output_dirpath, - sbatch_filepath_predict, - sbatch_filepath_preprocess, + sbatch_filepath, sbatch_to_submitit, ) -from biahub.cli.utils import get_submitit_cluster +from biahub.cli.utils import ( + get_submitit_cluster, + resolve_ome_zarr_version, +) -def run_viscy_preprocess( - data_path: str, - num_workers: int = 32, - config_file: str = None, - path_viscy_env: Path = None, - verbose: bool = False, -): - """ - Run VisCy preprocess on a single FOV. +def build_predict_parser(): + """Build a jsonargparse parser that mirrors the ``viscy predict`` config. - Parameters - ---------- - data_path : str - Path to the FOV data. - num_workers : int - Number of workers to use. - config_file : str - Path to the VisCy config file. - path_viscy_env : Path - Path to the VisCy environment. - verbose : bool - Whether to print verbose output. + The model and data blocks are registered as subclass arguments of VisCy's + own ``VSUNet`` and ``HCSDataModule`` classes, so the configuration is + validated against VisCy's actual class signatures -- the same machinery the + ``viscy predict`` CLI uses. biahub therefore keeps no copy of the model + schema that could fall out of date when VisCy changes upstream. + + The biahub-specific orchestration knobs (``sliding_window_step``, + ``device``, ``output_ome_zarr_version``) and the top-level ``ckpt_path`` + are added as plain arguments. ``data.init_args.data_path`` is intentionally + left out of the config file -- biahub injects the position path per job. """ - cmd = ( - "module load anaconda && " - f"conda activate {path_viscy_env} && " - f"viscy preprocess " - f"--data_path {data_path} " - f"--num_workers {num_workers} " + import jsonargparse + + from cytoland.engine import VSUNet + from viscy_data.hcs import HCSDataModule + + parser = jsonargparse.ArgumentParser() + parser.add_argument("--config", action="config") + parser.add_subclass_arguments(VSUNet, "model") + parser.add_subclass_arguments(HCSDataModule, "data") + parser.add_argument("--ckpt_path", type=str, required=True) + parser.add_argument("--sliding_window_step", type=int, default=1) + parser.add_argument("--device", type=str, default="cuda") + # Output OME-Zarr version: None preserves the input store's version. + parser.add_argument( + "--output_ome_zarr_version", + type=Literal["0.4", "0.5"] | None, + default=None, ) - if config_file: - cmd += f" -c {config_file}" - else: - cmd += "--channel_names -1 --block_size 32" - if verbose: - click.echo(f"Preprocess FOV: {'/'.join(Path(data_path).parts[-3:])}") - click.echo(f"Command: {cmd}") - subprocess.run(cmd, shell=True, check=True, executable="/bin/bash") - - -def run_viscy_predict( - data_path: str, - config_file: str, - output_store: str, - log_dir: str, - path_viscy_env: Path = None, - verbose: bool = False, -): - """ - Run VisCy predict on a single FOV. + return parser + + +def load_predict_config(config_filepath: Path, data_path: Path): + """Validate a VisCy predict config and inject the per-position ``data_path``. Parameters ---------- - data_path : str - Path to the FOV data. - config_file : str - Path to the VisCy config file. - output_store : str - Path to the output store. - log_dir : str - Path to the log directory. - path_viscy_env : Path - Path to the VisCy environment. - verbose : bool - Whether to print verbose output. - + config_filepath : Path + Path to the VisCy-style predict YAML config. + data_path : Path + Position path injected as ``data.init_args.data_path`` (biahub supplies + this per job; the first position is used for submit-time validation). + + Returns + ------- + tuple + ``(parser, cfg)`` where ``cfg`` is the validated config namespace. """ - os.chdir(log_dir) - # Compose the shell command - cmd = ( - "module load anaconda && " - f"conda activate {path_viscy_env} && " - "viscy predict " - f'-c "{config_file}" ' - f'--data.init_args.data_path "{data_path}" ' - f"--trainer.callbacks+=viscy.translation.predict_writer.HCSPredictionWriter " - f'--trainer.callbacks.output_store "{output_store}" ' - f'--trainer.default_root_dir "{log_dir}"' + parser = build_predict_parser() + cfg = parser.parse_args( + [ + "--config", + str(config_filepath), + "--data.init_args.data_path", + str(data_path), + ] ) - if verbose: - click.echo(f"Predict FOV: {'/'.join(Path(data_path).parts[-3:])}") - click.echo(f"Command: {cmd}") - subprocess.run(cmd, shell=True, check=True, executable="/bin/bash") + return parser, cfg -def combine_fov_zarrs_to_plate( - fovs: list[Path], - temp_dir: Path, - output_dirpath: Path, - cleanup: bool = True, +def virtual_stain_position( + config_filepath: Path, + input_position_path: Path, + output_position_path: Path, ): - """ - Combine VisCy-predicted FOV Zarrs (in temp) into a single HCS plate Zarr by moving. + """Run cytoland virtual staining on a single position, looping over time. + + Inference is GPU-bound, so timepoints are processed serially in a ``for`` + loop rather than in parallel. Each timepoint volume is normalized with the + config's ``NormalizeSampled`` transforms (using the precomputed statistics + in the store) and passed through + ``AugmentedPredictionVSUNet.predict_sliding_windows`` -- the same linear + feathering blend used by the ``viscy predict`` ``HCSPredictionWriter``. + + Normalization and test-time augmentation are delegated to VisCy + (``NormalizeSampled`` / ``read_norm_meta`` / ``with_rotation_tta``) so the + behavior stays in sync with ``viscy predict``. Parameters ---------- - fovs : list of Path - Original FOV paths (used to extract B/1/000000). - temp_dir : Path - Directory containing the individual .zarr folders (each named like B_1_000000.zarr). - output_dirpath : Path - The plate-level HCS Zarr to merge into. - cleanup : bool - Whether to delete the moved files afterwards. Default True. + config_filepath : Path + Path to the VisCy-style predict config. + input_position_path : Path + Input position (label-free source) to virtually stain. + output_position_path : Path + Output position to write predictions into. """ - for fov in fovs: - row, col, pos = fov.parts[-3:] - nested_fov_path = temp_dir / f"{row}_{col}_{pos}.zarr" / row / col / pos + from cytoland.engine import AugmentedPredictionVSUNet + from monai.transforms import Compose + from viscy_data import read_norm_meta + + position_name = "/".join(Path(input_position_path).parts[-3:]) + position_start = time.perf_counter() + parser, cfg = load_predict_config(config_filepath, input_position_path) + + device = torch.device( + cfg.device if (cfg.device == "cpu" or torch.cuda.is_available()) else "cpu" + ) + click.echo(f"[{position_name}] Starting virtual staining on device '{device}'") + + # Instantiate the VSUNet and the data module from VisCy's own classes. + # Route the top-level ckpt_path into the model's init args so VSUNet loads + # the weights itself: VSUNet.__init__ extracts the Lightning checkpoint's + # "state_dict" exactly as `viscy predict` does. Delegating keeps biahub free + # of both the model schema and any assumption about the checkpoint layout. + cfg.model.init_args.ckpt_path = str(cfg.ckpt_path) + instances = parser.instantiate(cfg) + vsunet = instances.model + vsunet.eval().to(device) + click.echo(f"[{position_name}] Loaded checkpoint: {cfg.ckpt_path}") + + # Test-time augmentation: reuse VisCy's rotation-TTA helper, which is + # correct for non-square FOVs. Defaults follow the config's model flags. + use_tta = bool(getattr(cfg.model.init_args, "test_time_augmentations", False)) + tta_type = getattr(cfg.model.init_args, "tta_type", "mean") + reduction = "median" if tta_type == "median" else "mean" + if use_tta: + predictor = AugmentedPredictionVSUNet.with_rotation_tta( + vsunet.model, reduction=reduction + ) + click.echo( + f"[{position_name}] Test-time augmentation: on ({reduction} over 4 rotations)" + ) + else: + predictor = AugmentedPredictionVSUNet(model=vsunet.model) + click.echo(f"[{position_name}] Test-time augmentation: off") + predictor = predictor.eval().to(device) + + # Normalization: reuse the configured NormalizeSampled transforms directly. + normalize = Compose(instances.data.normalizations) + + source_channel = cfg.data.init_args.source_channel + source_channel = source_channel[0] if isinstance(source_channel, list) else source_channel + target_channels = cfg.data.init_args.target_channel + target_channels = ( + [target_channels] if isinstance(target_channels, str) else target_channels + ) + out_channel = len(target_channels) + step = cfg.sliding_window_step + + with open_ome_zarr(str(input_position_path), mode="r") as input_dataset: + T, _, Z, Y, X = input_dataset.data.shape + channel_index = input_dataset.channel_names.index(source_channel) + # Precomputed statistics written by `viscy preprocess`. + norm_meta = read_norm_meta(input_dataset) + if norm_meta is None or source_channel not in norm_meta: + raise RuntimeError( + f"Normalization statistics for channel '{source_channel}' were not " + f"found in the input store. Run `viscy preprocess` on the input " + f"dataset before virtual staining." + ) + + # Depth of the Z-window fed to the model. In VisCy's own + # `predict_sliding_windows`, `model.out_stack_depth` is the input window + # depth: each window is `x[:, :, start:start + out_stack_depth]`. We + # mirror that here purely to log the window count (the value matches + # `len(range(0, Z - out_stack_depth + 1, step))` exactly). "?" when the + # model exposes no such attribute, and 0 when the volume is too shallow + # for even one window (predict_sliding_windows then raises). + z_window_depth = getattr(vsunet.model, "out_stack_depth", None) + n_windows = max(0, (Z - z_window_depth) // step + 1) if z_window_depth else "?" + click.echo( + f"[{position_name}] {T} timepoints, volume (Z,Y,X)=({Z},{Y},{X}), " + f"'{source_channel}' -> {target_channels}, " + f"{n_windows} sliding windows/timepoint (step={step})" + ) + + with open_ome_zarr(str(output_position_path), mode="r+") as output_dataset: + for t in range(T): + t_start = time.perf_counter() + # (1, 1, Z, Y, X) source volume for this timepoint + volume = np.asarray( + input_dataset.data[t : t + 1, channel_index : channel_index + 1] + ) + source = torch.from_numpy(volume).float().to(device) + # Apply VisCy's configured normalization via a sample dict. + sample = normalize({source_channel: source, "norm_meta": norm_meta}) + source = sample[source_channel] + with torch.inference_mode(): + prediction = predictor.predict_sliding_windows( + source, out_channel=out_channel, step=step + ) + output_dataset.data[t] = prediction[0].cpu().numpy() + click.echo( + f"[{position_name}] timepoint {t + 1}/{T} done " + f"({time.perf_counter() - t_start:.1f}s)" + ) + + click.echo( + f"[{position_name}] Completed {T} timepoints in " + f"{time.perf_counter() - position_start:.1f}s -> {output_position_path}" + ) - if not nested_fov_path.exists(): - print(f"Skipping missing: {nested_fov_path}") - continue - dest_path = output_dirpath / row / col / pos +def _init_output_plate( + input_position_dirpaths: list[Path], + output_dirpath: Path, + target_channels: list[str], + output_ome_zarr_version: str | None = None, +) -> tuple[int, int, int, int, int]: + """Create (or extend) the empty virtual-stain output plate. - if dest_path.exists(): - shutil.rmtree(dest_path) + The output mirrors the input geometry but contains only the predicted + virtual-stain channels. ``create_empty_plate`` is idempotent, so this is + safe to call from both the orchestrator and per-position runs. - print(f"Moving {nested_fov_path} → {dest_path}") - shutil.move(str(nested_fov_path), str(dest_path)) + ``output_ome_zarr_version`` dictates the output store's OME-Zarr version; + when None the input store's version is preserved. - # Optionally remove the full temp zarr folder if it's now empty - if cleanup: - try: - shutil.rmtree(temp_dir) - except Exception as e: - print(f"Could not remove {temp_dir}: {e}") + Returns the input ``(T, C, Z, Y, X)`` shape. + """ + with open_ome_zarr(str(input_position_dirpaths[0]), mode="r") as input_dataset: + input_shape = input_dataset.data.shape + scale = input_dataset.scale + T, C, Z, Y, X = input_shape + + create_empty_plate( + store_path=output_dirpath, + position_keys=[Path(p).parts[-3:] for p in input_position_dirpaths], + channel_names=target_channels, + shape=(T, len(target_channels), Z, Y, X), + scale=scale, + version=resolve_ome_zarr_version(input_position_dirpaths[0], output_ome_zarr_version), + ) - print(f"Combined all FOVs into {output_dirpath}") + return (T, C, Z, Y, X) def virtual_stain( input_position_dirpaths: list[str], + config_filepath: Path, output_dirpath: str, - predict_config_filepath: str, - path_viscy_env: str, - preprocess_config_filepath: str = None, - sbatch_filepath_preprocess: str = None, - sbatch_filepath_predict: str = None, - run_mode: str = "all", - num_processes: int = 32, - local: bool = False, + sbatch_filepath: str = None, + cluster: str = "slurm", monitor: bool = True, - verbose: bool = True, + init_only: bool = False, ): - """ - Run VisCy virtual stain on a plate. + """Run cytoland virtual staining on a plate, one GPU job per position. + + Opens the input store, creates an output store containing only the + predicted virtual-stain channels, and dispatches one job per position. Each + job runs ``virtual_stain_position``, which loops over timepoints on a single + GPU. Parameters ---------- - input_position_dirpaths : List[str] - List of paths to the input position directories. + input_position_dirpaths : list[str] + Input position directory paths. + config_filepath : Path + Path to the VisCy-style predict config. output_dirpath : str - Path to the output directory. - predict_config_filepath : str - Path to the VisCy predict config file. - preprocess_config_filepath : str - Path to the VisCy preprocess config file. - path_viscy_env : str - Path to the VisCy environment. - sbatch_filepath_preprocess : str - Path to the VisCy preprocess sbatch file. - sbatch_filepath_predict : str - Path to the VisCy predict sbatch file. - run_mode : str - Which VisCy stage(s) to run. - num_processes : int - Number of processes to use. - local : bool - Whether to run locally. - monitor : bool - + Output plate path. + sbatch_filepath : str, optional + SBATCH file overriding default SLURM parameters. + cluster : str, optional + Execution cluster: 'slurm' submits to a Slurm cluster, 'local' runs jobs + as subprocesses on this machine, 'debug' runs jobs in-process in the + foreground. + monitor : bool, optional + Monitor submitted jobs. + init_only : bool, optional + Only initialize the output store and exit; skip per-position processing. """ + # The 'local' cluster launches every position as a concurrent subprocess + # (submitit's LocalExecutor ignores `slurm_array_parallelism`, so there is no + # concurrency cap) and biahub does not pin per-job GPUs, so all jobs target + # cuda:0. With more than one position that oversubscribes a single GPU. Use + # 'slurm' for multi-GPU parallelism or 'debug' to run positions one at a time. + if cluster == "local" and len(input_position_dirpaths) > 1: + raise ValueError( + f"cluster='local' cannot run {len(input_position_dirpaths)} positions: " + "local jobs run concurrently without a parallelism cap and all target " + "the same GPU. Use cluster='slurm' for multi-GPU parallelism, or " + "cluster='debug' to run positions sequentially on this machine." + ) + output_dirpath = Path(output_dirpath) + config_filepath = Path(config_filepath) slurm_out_path = output_dirpath.parent / "slurm_output" - cluster = get_submitit_cluster(local) - - job_ids_preprocess = [] - slurm_dependency = None - path_viscy_env = Path(path_viscy_env) - - if run_mode in ["all", "preprocess"]: - slurm_args_preprocess = { - "slurm_job_name": "VS_preprocess", - "slurm_mem_per_cpu": "8G", - "slurm_cpus_per_task": num_processes, - "slurm_array_parallelism": 1, - "slurm_time": 8 * 60, - "slurm_partition": "cpu", - } - - if sbatch_filepath_preprocess: - slurm_args_preprocess.update(sbatch_to_submitit(sbatch_filepath_preprocess)) - - executor = submitit.AutoExecutor(folder=slurm_out_path / "preprocess", cluster=cluster) - executor.update_parameters(**slurm_args_preprocess) - click.echo(f"Submitting preprocess job with: {slurm_args_preprocess}") - - plate_path = Path(input_position_dirpaths[0]).parents[2] - with executor.batch(): - job = executor.submit( - run_viscy_preprocess, - data_path=str(plate_path), - num_workers=num_processes, - config_file=preprocess_config_filepath, - path_viscy_env=path_viscy_env, - verbose=verbose, - ) - job_ids_preprocess.append(job) - - job_ids = [ - job.job_id for job in job_ids_preprocess - ] # Access job IDs after batch submission - - log_path = Path(slurm_out_path / "preprocess" / "submitit_jobs_ids.log") - log_path.parent.mkdir(parents=True, exist_ok=True) - with log_path.open("w") as log_file: - log_file.write("\n".join(job_ids)) - - slurm_dependency = f"afterok:{job.job_id}" - - job_ids_predict = [] - job_ids_combine = [] - - if run_mode in ["all", "predict"]: - slurm_args_predict = { - "slurm_job_name": "VS_predict", - "slurm_mem_per_cpu": "16G", - "slurm_cpus_per_task": 32, - "slurm_array_parallelism": 100, - "slurm_time": 8 * 60, - "slurm_partition": "gpu", - "slurm_gres": "gpu:1", - "slurm_constraint": "a100|a6000|a40", - "slurm_use_srun": False, - } - - if slurm_dependency: - slurm_args_predict["slurm_dependency"] = slurm_dependency - - if sbatch_filepath_predict: - slurm_args_predict.update(sbatch_to_submitit(sbatch_filepath_predict)) - - executor = submitit.AutoExecutor(folder=slurm_out_path / "predict", cluster=cluster) - executor.update_parameters(**slurm_args_predict) - click.echo(f"Submitting predict jobs with: {slurm_args_predict}") - config_file = str(Path(predict_config_filepath).resolve()) - fovs = [] - - with executor.batch(): - for input_position_path in input_position_dirpaths: - fov = Path(*input_position_path.parts[-3:]) - fovs.append(fov) - log_dir = (output_dirpath.parent / "logs" / "_".join(fov.parts)).resolve() - os.makedirs(log_dir, exist_ok=True) - data_path = str(Path(input_position_path).resolve()) - - output_fov_path = ( - output_dirpath.parent / "temp" / f"{'_'.join(fov.parts)}.zarr" - ) - output_store = str(Path(output_fov_path).resolve()) - - job = executor.submit( - run_viscy_predict, - data_path=data_path, - config_file=config_file, - output_store=output_store, - log_dir=log_dir, - path_viscy_env=path_viscy_env, - verbose=verbose, - ) - job_ids_predict.append(job) - - job_ids = [ - job.job_id for job in job_ids_predict - ] # Access job IDs after batch submission - - log_path = Path(slurm_out_path / "predict" / "submitit_jobs_ids.log") - log_path.parent.mkdir(parents=True, exist_ok=True) - with log_path.open("w") as log_file: - log_file.write("\n".join(job_ids)) - - with open_ome_zarr(input_position_dirpaths[0]) as dataset: - T, C, Z, Y, X = dataset.data.shape - channel_names = dataset.channel_names - scale = dataset.scale - - output_metadata = { - "shape": (T, len(channel_names), Z, Y, X), - "chunks": None, - "scale": scale, - "channel_names": channel_names, - "dtype": np.float32, - } - - create_empty_plate( - store_path=output_dirpath, - position_keys=[p.parts[-3:] for p in input_position_dirpaths], - **output_metadata, - ) + # Validate the config against VisCy's schema up front (using the first + # position for data_path) and read the predicted channel names. + _, cfg = load_predict_config(config_filepath, input_position_dirpaths[0]) + target_channels = cfg.data.init_args.target_channel + target_channels = ( + [target_channels] if isinstance(target_channels, str) else target_channels + ) - slurm_args_combine = { - "slurm_job_name": "VS_combine", - "slurm_mem_per_cpu": "8G", - "slurm_cpus_per_task": num_processes, - "slurm_array_parallelism": 1, - "slurm_time": 8 * 60, - "slurm_partition": "cpu", - "slurm_dependency": f"afterok:{':'.join([str(job.job_id) for job in job_ids_predict])}", - } - - executor = submitit.AutoExecutor(folder=slurm_out_path / "combine", cluster=cluster) - executor.update_parameters(**slurm_args_combine) - click.echo(f"Submitting combine job with: {slurm_args_combine}") - - plate_path = Path(input_position_dirpaths[0]).parents[2] - - with executor.batch(): - job = executor.submit( - combine_fov_zarrs_to_plate, - fovs=fovs, - temp_dir=output_dirpath.parent / "temp", - output_dirpath=output_dirpath, - cleanup=True, + input_shape = _init_output_plate( + input_position_dirpaths, + output_dirpath, + target_channels, + cfg.output_ome_zarr_version, + ) + + # Timepoints are processed sequentially on a single GPU, so resource needs + # are independent of dataset size. + num_cpus, gb_ram = 16, 64 + click.echo(f"RESOURCES:{num_cpus} {gb_ram}") + + if init_only: + click.echo(f"Initialized {output_dirpath} ({len(input_position_dirpaths)} positions)") + return + + output_position_paths = utils.get_output_paths(input_position_dirpaths, output_dirpath) + + T, Z = input_shape[0], input_shape[2] + # Generous wall-clock budget (minutes), assuming median TTA (4 rotations). + # Each timepoint runs ~Z sliding windows along Z. Measured ~1.4 s/window + # with TTA on this model; budget ~5 s/window (~3-4x margin for slower GPUs, + # larger FOVs, and compute-bound runs) with a 60-minute floor. + seconds_per_window = 5 + slurm_time = int(np.ceil(max(60, T * Z * seconds_per_window / 60))) + # Prepare SLURM arguments + slurm_args = { + "slurm_job_name": "virtual-stain", + "slurm_gres": "gpu:1", + "slurm_mem_per_cpu": f"{gb_ram}G", + "slurm_cpus_per_task": num_cpus, + "slurm_array_parallelism": 20, # process up to 20 positions at a time + "slurm_time": slurm_time, + "slurm_partition": "gpu", + } + + # Override defaults if sbatch_filepath is provided + if sbatch_filepath: + slurm_args.update(sbatch_to_submitit(sbatch_filepath)) + + resolved_cluster = get_submitit_cluster(cluster=cluster) + click.echo(f"Preparing jobs on cluster='{resolved_cluster}': {slurm_args}") + executor = submitit.AutoExecutor(folder=slurm_out_path, cluster=resolved_cluster) + executor.update_parameters(**slurm_args) + + click.echo("Submitting jobs...") + jobs = [] + with submitit.helpers.clean_env(), executor.batch(): + for input_position_path, output_position_path in zip( + input_position_dirpaths, output_position_paths, strict=True + ): + jobs.append( + executor.submit( + virtual_stain_position, + config_filepath, + input_position_path, + output_position_path, + ) ) - job_ids_combine.append(job) - job_ids = [ - job.job_id for job in job_ids_combine - ] # Access job IDs after batch submission + job_ids = [job.job_id for job in jobs] # Access job IDs after batch submission + + slurm_out_path.mkdir(parents=True, exist_ok=True) + log_path = Path(slurm_out_path / "submitit_jobs_ids.log") + with log_path.open("w") as log_file: + log_file.write("\n".join(job_ids)) - log_path = Path(slurm_out_path / "combine" / "submitit_jobs_ids.log") - log_path.parent.mkdir(parents=True, exist_ok=True) - with log_path.open("w") as log_file: - log_file.write("\n".join(job_ids)) + # submitit's DebugExecutor is lazy: .submit() wraps the callable in a + # DebugJob but execution only happens when .wait()/.done()/.result() is + # called. Run each one in the foreground and stream progress; monitor's + # async polling UI is pointless against synchronous in-process jobs. + if resolved_cluster == "debug": + for job, path in zip(jobs, input_position_dirpaths, strict=True): + job.wait() + click.echo(f"Virtual staining complete: {path}") + return if monitor: - job_ids = [ - job.job_id for job in job_ids_predict + job_ids_preprocess + job_ids_combine - ] - monitor_jobs(job_ids, input_position_dirpaths) + monitor_jobs(jobs, input_position_dirpaths) @click.command("virtual-stain") @input_position_dirpaths() +@config_filepath() @output_dirpath() -@sbatch_filepath_preprocess() -@sbatch_filepath_predict() -@num_processes() -@local() +@sbatch_filepath() +@cluster() @monitor() -@click.option("--verbose", is_flag=True, default=False, help="Verbose output.") -@click.option( - "--path-viscy-env", - required=True, - help="Conda environment with VisCy installed.", -) -@click.option( - "--preprocess-config-filepath", - type=str, - help="Path to the VisCy preprocess config file.", -) -@click.option( - "--predict-config-filepath", - type=str, - help="Path to the VisCy predict config file.", -) -@click.option( - "--run-mode", - type=click.Choice(["all", "preprocess", "predict"]), - default="all", - help="Which VisCy stage(s) to run.", -) +@init_only() def virtual_stain_cli( input_position_dirpaths: list[str], + config_filepath: Path, output_dirpath: str, - predict_config_filepath: str, - path_viscy_env: str, - preprocess_config_filepath: str = None, - run_mode: str = "all", - num_processes: int = 32, - sbatch_filepath_preprocess: str = None, - sbatch_filepath_predict: str = None, - local: bool = False, - monitor: bool = True, - verbose: bool = True, + sbatch_filepath: str = None, + cluster: str = "slurm", + monitor: bool = False, + init_only: bool = False, ): - """Run VisCy virtual staining on a zarr plate from dedicated python environment. - - >>> biahub virtual-stain \ - --input-position-dirpaths path.zarr/*/*/* \ - --output-dirpath output.zarr \ - --predict-config-filepath predict.yml \ - --preprocess-config-filepath preprocess.yml \ - --path-viscy-env /path/to/viscy/env \ - --run-mode all - """ + """Virtually stain a label-free dataset using a cytoland (VisCy) model. + + Runs cytoland's ``predict_sliding_windows`` per position, looping over + timepoints on a single GPU. The config is a ``viscy predict`` YAML and is + validated against VisCy's own model/data classes. + + \b + SLURM fan-out of positions across a whole plate: + >>> biahub virtual-stain -i ./input.zarr/*/*/* -c ./virtual_stain_params.yml -o ./output.zarr + + \b + Initialize the output plate only (e.g. before running per-position Nextflow workers): + >>> biahub virtual-stain --init -i ./input.zarr/*/*/* -c ./virtual_stain_params.yml -o ./output.zarr + + \b + In-process run of a single position (e.g. from a Nextflow worker): + >>> biahub virtual-stain --cluster debug -i ./input.zarr/A/1/0 -c ./virtual_stain_params.yml -o ./output.zarr + """ # noqa: D301 virtual_stain( input_position_dirpaths=input_position_dirpaths, + config_filepath=config_filepath, output_dirpath=output_dirpath, - predict_config_filepath=predict_config_filepath, - preprocess_config_filepath=preprocess_config_filepath, - sbatch_filepath_preprocess=sbatch_filepath_preprocess, - sbatch_filepath_predict=sbatch_filepath_predict, - path_viscy_env=path_viscy_env, - run_mode=run_mode, - num_processes=num_processes, - local=local, + sbatch_filepath=sbatch_filepath, + cluster=cluster, monitor=monitor, - verbose=verbose, + init_only=init_only, ) diff --git a/pyproject.toml b/pyproject.toml index 1a61abe2..39a7bdbb 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -52,10 +52,7 @@ track = ["ultrack>=0.7"] gui = ["napari[pyqt6]", "PyQt6>=6.5,<6.8", "napari-animation"] -# TODO: resolved to v0.3.2 which is not compatible with iohub 0.3.0a7 -# bring bach once we sort out iohub dependencies -# stain = ["viscy"] -stain = [] +stain = ["cytoland"] build = ["build", "twine"] @@ -84,6 +81,9 @@ biahub = "biahub.cli.main:cli" index-strategy = "unsafe-best-match" [tool.uv.sources] +# Pin cytoland to the commit from VisCy PR #465 (non-square TTA and helpers). +# Revert to the PyPI release once that PR is merged and published. +cytoland = { git = "https://github.com/mehta-lab/VisCy", subdirectory = "applications/cytoland", rev = "7fb3bc1e78c0e0bd26e1845caba860f76223ba02" } # Pin iohub to the branch that stores per-step provenance as top-level # zattrs keys (e.g. biahub-flat_field, biahub-deskew). Revert to the PyPI # release once those changes are merged and published. diff --git a/settings/example_virtual_stain_settings.yml b/settings/example_virtual_stain_settings.yml new file mode 100644 index 00000000..206763aa --- /dev/null +++ b/settings/example_virtual_stain_settings.yml @@ -0,0 +1,44 @@ +# Virtual staining (cytoland / VisCy) predict config for `biahub virtual-stain`. +# This is a `viscy predict` config and is validated against VisCy's own +# VSUNet / HCSDataModule classes. `data.init_args.data_path` is injected by +# biahub per position, so it is omitted here. +model: + class_path: cytoland.engine.VSUNet + init_args: + architecture: fcmae + model_config: + in_channels: 1 + out_channels: 2 + in_stack_depth: 15 + encoder_blocks: [3, 3, 9, 3] + dims: [96, 192, 384, 768] + decoder_conv_blocks: 2 + stem_kernel_size: [5, 4, 4] + pretraining: false + test_time_augmentations: true + tta_type: median + +data: + class_path: viscy_data.hcs.HCSDataModule + init_args: + source_channel: Phase3D + target_channel: [nuclei, membrane] + z_window_size: 15 # must match model in_stack_depth + # No effect on biahub: predict_sliding_windows processes one z-window at a + # time. Kept for parity with `viscy predict` configs. + batch_size: 1 + normalizations: + - class_path: viscy_transforms.NormalizeSampled + init_args: + keys: [Phase3D] + level: fov_statistics + subtrahend: median + divisor: iqr + +ckpt_path: /hpc/projects/comp.micro/virtual_staining/models/fcmae-cyto3d-sensor/vscyto3d-logs/hek-a549-ipsc-finetune/checkpoints/epoch=83-step=14532-loss=0.492.ckpt + +# Optional biahub orchestration knobs (defaults shown). +sliding_window_step: 1 # Z step for the sliding window; 1 = maximum overlap. +device: cuda # torch device for inference. +# Output OME-Zarr version: null preserves the input store's version; "0.4" or "0.5" overrides. +output_ome_zarr_version: null diff --git a/tests/test_example_settings.py b/tests/test_example_settings.py index f3e48fef..e0f12453 100644 --- a/tests/test_example_settings.py +++ b/tests/test_example_settings.py @@ -55,6 +55,12 @@ ("example_flat_field_settings.yml", FlatFieldCorrectionSettings), ] +# Example configs validated against VisCy's own classes via jsonargparse rather +# than a biahub pydantic Settings model (virtual staining keeps no model schema). +jsonargparse_settings_files = [ + "example_virtual_stain_settings.yml", +] + try: import cellpose # noqa: F401 @@ -62,17 +68,38 @@ except ImportError: cellpose_available = False +try: + import cytoland # noqa: F401 + + cytoland_available = True +except ImportError: + cytoland_available = False + def test_all_example_settings_tested(): num_settings_files = len( list(settings_files_dir.glob("*.yml")) + list(settings_files_dir.glob("*.yaml")) ) - assert num_settings_files == len(example_settings_params), ( + num_tested = len(example_settings_params) + len(jsonargparse_settings_files) + assert num_settings_files == num_tested, ( "Not all example settings files are tested. " - f"Found {num_settings_files} files, but {len(example_settings_params)} are tested in test_example_settings." + f"Found {num_settings_files} files, but {num_tested} are tested in test_example_settings." ) +@pytest.mark.parametrize("path", jsonargparse_settings_files) +def test_example_jsonargparse_settings(path): + # These configs are validated against VisCy's VSUNet/HCSDataModule classes, + # which require the optional `stain` extra (cytoland). + if not cytoland_available: + pytest.skip("cytoland not installed; skipping VisCy-config validation.") + + from biahub.virtual_stain import load_predict_config + + # data_path is injected per position at runtime; any placeholder validates. + load_predict_config(settings_files_dir / path, Path("/placeholder.zarr/A/1/0")) + + @pytest.mark.parametrize("path,settings_cls", example_settings_params) def test_example_settings(path, settings_cls): # Skip test if cellpose isn't installed and we're testing SegmentationSettings diff --git a/uv.lock b/uv.lock index 26a5ec62..d50bb4ac 100644 --- a/uv.lock +++ b/uv.lock @@ -2,18 +2,30 @@ version = 1 revision = 3 requires-python = ">=3.12" resolution-markers = [ - "python_full_version >= '3.14' and sys_platform == 'win32'", - "python_full_version >= '3.14' and sys_platform == 'emscripten'", - "python_full_version >= '3.14' and sys_platform == 'darwin'", - "python_full_version >= '3.14' and sys_platform != 'darwin' and sys_platform != 'emscripten' and sys_platform != 'win32'", - "python_full_version == '3.13.*' and sys_platform == 'win32'", - "python_full_version < '3.13' and sys_platform == 'win32'", - "python_full_version == '3.13.*' and sys_platform == 'emscripten'", - "python_full_version < '3.13' and sys_platform == 'emscripten'", - "python_full_version == '3.13.*' and sys_platform == 'darwin'", - "python_full_version < '3.13' and sys_platform == 'darwin'", - "python_full_version == '3.13.*' and sys_platform != 'darwin' and sys_platform != 'emscripten' and sys_platform != 'win32'", - "python_full_version < '3.13' and sys_platform != 'darwin' and sys_platform != 'emscripten' and sys_platform != 'win32'", + "python_full_version >= '3.14' and platform_machine != 's390x' and sys_platform == 'win32'", + "python_full_version >= '3.14' and platform_machine == 's390x' and sys_platform == 'win32'", + "python_full_version >= '3.14' and platform_machine != 's390x' and sys_platform == 'emscripten'", + "python_full_version >= '3.14' and platform_machine == 's390x' and sys_platform == 'emscripten'", + "python_full_version >= '3.14' and platform_machine != 's390x' and sys_platform == 'darwin'", + "python_full_version >= '3.14' and platform_machine == 's390x' and sys_platform == 'darwin'", + "python_full_version >= '3.14' and platform_machine != 's390x' and sys_platform != 'darwin' and sys_platform != 'emscripten' and sys_platform != 'win32'", + "python_full_version >= '3.14' and platform_machine == 's390x' and sys_platform != 'darwin' and sys_platform != 'emscripten' and sys_platform != 'win32'", + "python_full_version == '3.13.*' and platform_machine != 's390x' and sys_platform == 'win32'", + "python_full_version == '3.13.*' and platform_machine == 's390x' and sys_platform == 'win32'", + "python_full_version < '3.13' and platform_machine != 's390x' and sys_platform == 'win32'", + "python_full_version < '3.13' and platform_machine == 's390x' and sys_platform == 'win32'", + "python_full_version == '3.13.*' and platform_machine != 's390x' and sys_platform == 'emscripten'", + "python_full_version == '3.13.*' and platform_machine == 's390x' and sys_platform == 'emscripten'", + "python_full_version < '3.13' and platform_machine != 's390x' and sys_platform == 'emscripten'", + "python_full_version < '3.13' and platform_machine == 's390x' and sys_platform == 'emscripten'", + "python_full_version == '3.13.*' and platform_machine != 's390x' and sys_platform == 'darwin'", + "python_full_version == '3.13.*' and platform_machine == 's390x' and sys_platform == 'darwin'", + "python_full_version < '3.13' and platform_machine != 's390x' and sys_platform == 'darwin'", + "python_full_version < '3.13' and platform_machine == 's390x' and sys_platform == 'darwin'", + "python_full_version == '3.13.*' and platform_machine != 's390x' and sys_platform != 'darwin' and sys_platform != 'emscripten' and sys_platform != 'win32'", + "python_full_version == '3.13.*' and platform_machine == 's390x' and sys_platform != 'darwin' and sys_platform != 'emscripten' and sys_platform != 'win32'", + "python_full_version < '3.13' and platform_machine != 's390x' and sys_platform != 'darwin' and sys_platform != 'emscripten' and sys_platform != 'win32'", + "python_full_version < '3.13' and platform_machine == 's390x' and sys_platform != 'darwin' and sys_platform != 'emscripten' and sys_platform != 'win32'", ] [[package]] @@ -179,7 +191,7 @@ wheels = [ [[package]] name = "antspyx" -version = "0.6.3" +version = "0.6.1" source = { registry = "https://pypi.org/simple" } 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