Grammatical / typo errors in Trimming and Filtering

[anniewest]

In the Running Trimmomatic section, the following text contains typo and grammatical errors:

We will also use a sliding window of size 4 that will remove bases if their phred score is below 20 (like in our example above). We will also discard any reads that do not have at least 25 bases remaining after this trimming step. Three additional pieces of code are also added to the end of the ILLUMINACLIP step. These three additional numbers (2:40:15) tell Trimmimatic how to handle sequence matches to the Nextera adapters. A detailed explanation of how they work is advanced for this particular lesson. For now we will use these numbers as a default and recognize they are needed to for Trimmomatic to run properly.

This could be revised to:

We will use a sliding window of size 4 that will remove bases if their phred score is below 20 (like in our example above). We will also discard any reads that do not have at least 25 bases remaining after this trimming step. An additional piece of code comprising of three numbers is also added to the end of the ILLUMINACLIP step. These three additional numbers (2:40:15) tell Trimmimatic how to handle sequence matches to the Nextera adapters. A detailed explanation of how they work is too advanced for this particular lesson, but please read the manual if you'd like more information regarding this step. For now, we will use these default numbers selected for this particular workshop and recognize they are needed for Trimmomatic to process our sequences appropriately.