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element_interface/suite2p_trigger.py

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@@ -6,15 +6,13 @@
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def motion_correction_suite2p(ops, db):
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"""Performs motion correction (i.e. registration) using the Suite2p package.
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Args:
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ops (dict): ops dictionary can be obtained by using `suite2p.default_ops()`
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function. It contains all options and default values used
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to perform preprocessing. ops['do_registration'] should be
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set to 1.
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db (dict): dictionary that includes paths pointing towards the input
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data, and path to store outputs
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Returns:
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motion_correction_ops (dict): Returns a dictionary that includes x and y shifts.
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A subset of the ops dictionary returned from `suite2p.run_s2p()` that is required for the segmentation step.
@@ -79,7 +77,6 @@ def motion_correction_suite2p(ops, db):
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def segmentation_suite2p(motion_correction_ops, db):
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"""Performs cell segmentation (i.e. roi detection) using Suite2p package.
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Args:
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motion_correction_ops (dict): options dictionary. Requirements:
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- x and y shifts
@@ -89,7 +86,6 @@ def segmentation_suite2p(motion_correction_ops, db):
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- spikedetect=False
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db (dict): dictionary that includes paths pointing towards the input
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data, and path to store outputs
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Returns:
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segmentation_ops (dict): A subset of the ops dictionary returned from `suite2p.run_s2p()` that is required for the deconvolution step.
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data.bin: Binary file if the one created during motion correction is deleted. If delete_bin=True, the binary file is deleted after processing.
@@ -141,7 +137,6 @@ def deconvolution_suite2p(segmentation_ops, db):
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"""Performs deconvolution using the Suite2p package for single plane tiff files.
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The code to run deconvolution separately can be found here
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</https://suite2p.readthedocs.io/en/latest/deconvolution.html>.
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Args:
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segmentation_ops (dict): options dictionary. Requirements:
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- baseline - setting that describes how to compute the baseline of each trace
@@ -156,7 +151,6 @@ def deconvolution_suite2p(segmentation_ops, db):
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- two_step_registration=False
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- roidetect=False
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- spikedetect=True
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Returns:
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spks.npy: Updates the file with an array of deconvolved traces
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"""

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