diff --git a/.changelog/5092.yml b/.changelog/5092.yml
new file mode 100644
index 0000000000..7f9a320a72
--- /dev/null
+++ b/.changelog/5092.yml
@@ -0,0 +1,4 @@
+changes:
+- description: Added validation AG104 to ensure the agent color is a valid 6-digit RGB hex format.
+  type: feature
+pr_number: 5092
diff --git a/demisto_sdk/commands/validate/sdk_validation_config.toml b/demisto_sdk/commands/validate/sdk_validation_config.toml
index 17daf64ffc..8a699c145f 100644
--- a/demisto_sdk/commands/validate/sdk_validation_config.toml
+++ b/demisto_sdk/commands/validate/sdk_validation_config.toml
@@ -162,6 +162,7 @@ select = [
   "AG101",
   "AG102",
   "AG103",
+  "AG104",
   "DS100",
   "DS101",
   "DS105",
@@ -323,6 +324,7 @@ select = [
   "AG101",
   "AG102",
   "AG103",
+  "AG104",
   "PA100",
   "PA101",
   "PA102",
diff --git a/demisto_sdk/commands/validate/tests/AG_validators_test.py b/demisto_sdk/commands/validate/tests/AG_validators_test.py
index 71c7b01ba5..c8fc053f05 100644
--- a/demisto_sdk/commands/validate/tests/AG_validators_test.py
+++ b/demisto_sdk/commands/validate/tests/AG_validators_test.py
@@ -3,12 +3,16 @@
 from demisto_sdk.commands.content_graph.objects.agentix_action import AgentixAction
 from demisto_sdk.commands.content_graph.objects.agentix_agent import AgentixAgent
 from demisto_sdk.commands.content_graph.objects.script import Script
+from demisto_sdk.commands.validate.tests.test_tools import create_agentix_agent_object
 from demisto_sdk.commands.validate.validators.AG_validators.AG100_is_forbidden_content_item import (
     IsForbiddenContentItemValidator,
 )
 from demisto_sdk.commands.validate.validators.AG_validators.AG101_is_correct_mp import (
     IsCorrectMPValidator,
 )
+from demisto_sdk.commands.validate.validators.AG_validators.AG104_is_valid_rgb_color import (
+    IsValidColorValidator,
+)
 
 
 def test_is_forbidden_content_item():
@@ -215,3 +219,51 @@ def test_is_correct_marketplace():
     assert results[0].message == (
         "The following Agentix related content item 'test' should have only marketplace 'platform'."
     )
+
+
+def test_is_valid_color():
+    """
+    Given:
+    - Two AgentixAgent items, one with a valid color and one with an invalid color.
+
+    When:
+    - Calling the IsValidColorValidator obtain_invalid_content_items function.
+
+    Then:
+    - Make sure one failure is returned for the invalid color and the error message is correct.
+    """
+    content_items = [
+        create_agentix_agent_object(
+            paths=["color", "display"],
+            values=["#FF0000", "Valid Color Agent"],
+        ),
+        create_agentix_agent_object(
+            paths=["color", "display"],
+            values=["invalid_color", "Invalid Color Agent"],
+        ),
+        create_agentix_agent_object(
+            paths=["color", "display"],
+            values=["#12345G", "Invalid Hex Agent"],
+        ),
+        create_agentix_agent_object(
+            paths=["color", "display"],
+            values=["#FFF", "Short Hex Agent"],
+        ),
+    ]
+
+    results = IsValidColorValidator().obtain_invalid_content_items(content_items)
+
+    assert len(results) == 3
+    error_messages = [result.message for result in results]
+    assert (
+        "The Agentix-agent 'Invalid Color Agent' color 'invalid_color' is not a valid RGB hex color.\n"
+        "Please make sure that the color is a valid 6-digit hex color string, starting with '#'. For example: '#FFFFFF'."
+    ) in error_messages
+    assert (
+        "The Agentix-agent 'Invalid Hex Agent' color '#12345G' is not a valid RGB hex color.\n"
+        "Please make sure that the color is a valid 6-digit hex color string, starting with '#'. For example: '#FFFFFF'."
+    ) in error_messages
+    assert (
+        "The Agentix-agent 'Short Hex Agent' color '#FFF' is not a valid RGB hex color.\n"
+        "Please make sure that the color is a valid 6-digit hex color string, starting with '#'. For example: '#FFFFFF'."
+    ) in error_messages
diff --git a/demisto_sdk/commands/validate/validators/AG_validators/AG104_is_valid_rgb_color.py b/demisto_sdk/commands/validate/validators/AG_validators/AG104_is_valid_rgb_color.py
new file mode 100644
index 0000000000..c383a11fe2
--- /dev/null
+++ b/demisto_sdk/commands/validate/validators/AG_validators/AG104_is_valid_rgb_color.py
@@ -0,0 +1,45 @@
+from __future__ import annotations
+
+import re
+from typing import Iterable, List
+
+from demisto_sdk.commands.common.constants import GitStatuses
+from demisto_sdk.commands.content_graph.objects.agentix_agent import AgentixAgent
+from demisto_sdk.commands.validate.validators.base_validator import (
+    BaseValidator,
+    ValidationResult,
+)
+
+ContentTypes = AgentixAgent
+
+
+class IsValidColorValidator(BaseValidator[ContentTypes]):
+    error_code = "AG104"
+    description = "Validate that the Agentix-agent color is a valid RGB hex color."
+    rationale = "The color field must be a valid RGB hex color string to be displayed correctly in the UI."
+    error_message = (
+        "The Agentix-agent '{0}' color '{1}' is not a valid RGB hex color.\n"
+        "Please make sure that the color is a valid 6-digit hex color string, starting with '#'. For example: '#FFFFFF'."
+    )
+    related_field = "color"
+    is_auto_fixable = False
+
+    def obtain_invalid_content_items(
+        self, content_items: Iterable[ContentTypes]
+    ) -> List[ValidationResult]:
+        return [
+            ValidationResult(
+                validator=self,
+                message=self.error_message.format(
+                    content_item.display_name,
+                    content_item.color,
+                ),
+                content_object=content_item,
+            )
+            for content_item in content_items
+            if not self.is_valid_color(content_item.color)
+        ]
+
+    def is_valid_color(self, color: str) -> bool:
+        """Checks if a string is a valid hex color."""
+        return bool(re.match(r"^#[0-9a-fA-F]{6}$", color))