diff --git a/src/System.Windows.Forms/System/Windows/Forms/ToolTip/ToolTip.cs b/src/System.Windows.Forms/System/Windows/Forms/ToolTip/ToolTip.cs
index 0078140bf83..4bdbb8da80d 100644
--- a/src/System.Windows.Forms/System/Windows/Forms/ToolTip/ToolTip.cs
+++ b/src/System.Windows.Forms/System/Windows/Forms/ToolTip/ToolTip.cs
@@ -718,6 +718,10 @@ private unsafe void CreateHandle()
             {
                 PInvoke.SetWindowTheme(HWND, string.Empty, string.Empty);
             }
+            else if (Application.IsDarkModeEnabled)
+            {
+                PInvoke.SetWindowTheme(HWND, $"{Control.DarkModeIdentifier}_{Control.ExplorerThemeIdentifier}", null);
+            }
         }
 
         // If in OwnerDraw mode, we don't want the default border.