diff --git a/packages/react-native/Libraries/Components/ScrollView/__tests__/ScrollView-viewCulling-itest.js b/packages/react-native/Libraries/Components/ScrollView/__tests__/ScrollView-viewCulling-itest.js
index 6bfbe37cc2527c..640dd5eee11483 100644
--- a/packages/react-native/Libraries/Components/ScrollView/__tests__/ScrollView-viewCulling-itest.js
+++ b/packages/react-native/Libraries/Components/ScrollView/__tests__/ScrollView-viewCulling-itest.js
@@ -1244,6 +1244,122 @@ describe('reparenting', () => {
     ]);
   });
 
+  test('flattening grandparent ', () => {
+    const root = Fantom.createRoot({viewportWidth: 100, viewportHeight: 100});
+
+    Fantom.runTask(() => {
+      root.render(
+        <ScrollView style={{height: 100, width: 100}}>
+          <View // grandparent
+            style={{
+              marginTop: 70,
+              opacity: 0, // opacity 0 - can't be flattened
+            }}>
+            <View // parent
+              nativeID="parent"
+              style={{height: 10, width: 10, marginTop: 10}}>
+              <View // child
+                nativeID="child"
+                style={{height: 5, width: 5, marginTop: 5}}
+              />
+            </View>
+          </View>
+        </ScrollView>,
+      );
+    });
+
+    expect(root.takeMountingManagerLogs()).toContain(
+      'Insert {type: "View", parentNativeID: "parent", index: 0, nativeID: "child"}',
+    );
+
+    // Flatten grandparent by changing opacity to default value.
+    Fantom.runTask(() => {
+      root.render(
+        <ScrollView style={{height: 100, width: 100}}>
+          <View // grandparent
+            style={{
+              marginTop: 70,
+            }}>
+            <View // parent
+              nativeID="parent"
+              style={{height: 10, width: 11, marginTop: 10}}>
+              <View // child
+                nativeID="child"
+                style={{height: 5, width: 5, marginTop: 5}}
+              />
+            </View>
+          </View>
+        </ScrollView>,
+      );
+    });
+
+    expect(root.takeMountingManagerLogs()).toEqual([
+      'Update {type: "View", nativeID: "parent"}',
+      'Remove {type: "View", parentNativeID: (N/A), index: 0, nativeID: "parent"}',
+      'Remove {type: "View", parentNativeID: (N/A), index: 0, nativeID: (N/A)}',
+      'Delete {type: "View", nativeID: (N/A)}',
+      'Insert {type: "View", parentNativeID: (N/A), index: 0, nativeID: "parent"}',
+    ]);
+  });
+
+  test('unflattening grandparent', () => {
+    const root = Fantom.createRoot({viewportWidth: 100, viewportHeight: 100});
+
+    Fantom.runTask(() => {
+      root.render(
+        <ScrollView style={{height: 100, width: 100}}>
+          <View // grandparent
+            style={{
+              marginTop: 70,
+            }}>
+            <View // parent
+              nativeID={'parent'}
+              style={{height: 10, width: 10, marginTop: 10}}>
+              <View // child
+                nativeID="child"
+                style={{height: 5, width: 5, marginTop: 5}}
+              />
+            </View>
+          </View>
+        </ScrollView>,
+      );
+    });
+
+    expect(root.takeMountingManagerLogs()).toContain(
+      'Insert {type: "View", parentNativeID: "parent", index: 0, nativeID: "child"}',
+    );
+
+    // Unflatten grandparent by setting opacity to 0.
+    Fantom.runTask(() => {
+      root.render(
+        <ScrollView style={{height: 100, width: 100}}>
+          <View // grandparent
+            style={{
+              marginTop: 70,
+              opacity: 0, // opacity 0 - can't be flattened
+            }}>
+            <View // parent
+              nativeID={'parent'}
+              style={{height: 10, width: 11, marginTop: 10}}>
+              <View // child
+                nativeID="child"
+                style={{height: 5, width: 5, marginTop: 5}}
+              />
+            </View>
+          </View>
+        </ScrollView>,
+      );
+    });
+
+    expect(root.takeMountingManagerLogs()).toEqual([
+      'Update {type: "View", nativeID: "parent"}',
+      'Remove {type: "View", parentNativeID: (N/A), index: 0, nativeID: "parent"}',
+      'Create {type: "View", nativeID: (N/A)}',
+      'Insert {type: "View", parentNativeID: (N/A), index: 0, nativeID: (N/A)}',
+      'Insert {type: "View", parentNativeID: (N/A), index: 0, nativeID: "parent"}',
+    ]);
+  });
+
   test('parent-child flattening with child culled', () => {
     const root = Fantom.createRoot({viewportWidth: 100, viewportHeight: 100});
     const nodeRef = createRef<HostInstance>();
diff --git a/packages/react-native/ReactCommon/react/renderer/mounting/Differentiator.cpp b/packages/react-native/ReactCommon/react/renderer/mounting/Differentiator.cpp
index acd3c7073c3d19..6aa22194ad166c 100644
--- a/packages/react-native/ReactCommon/react/renderer/mounting/Differentiator.cpp
+++ b/packages/react-native/ReactCommon/react/renderer/mounting/Differentiator.cpp
@@ -165,6 +165,7 @@ static void calculateShadowViewMutationsFlattener(
     Tag parentTagForUpdate,
     TinyMap<Tag, ShadowViewNodePair*>* parentSubVisitedOtherNewNodes,
     TinyMap<Tag, ShadowViewNodePair*>* parentSubVisitedOtherOldNodes,
+    const CullingContext& cullingContextForUnvisitedOtherNodes,
     const CullingContext& cullingContext);
 
 /**
@@ -221,6 +222,7 @@ static void updateMatchedPairSubtrees(
           oldPair.shadowView.tag,
           nullptr,
           nullptr,
+          oldCullingContext,
           oldCullingContextCopy);
     }
     // Unflattening
@@ -255,6 +257,7 @@ static void updateMatchedPairSubtrees(
           parentTag,
           nullptr,
           nullptr,
+          newCullingContext,
           newCullingContextCopy);
 
       // If old nodes were not visited, we know that we can delete
@@ -420,6 +423,7 @@ static void calculateShadowViewMutationsFlattener(
     Tag parentTagForUpdate,
     TinyMap<Tag, ShadowViewNodePair*>* parentSubVisitedOtherNewNodes,
     TinyMap<Tag, ShadowViewNodePair*>* parentSubVisitedOtherOldNodes,
+    const CullingContext& cullingContextForUnvisitedOtherNodes,
     const CullingContext& cullingContext) {
   // Step 1: iterate through entire tree
   std::vector<ShadowViewNodePair*> treeChildren =
@@ -615,10 +619,14 @@ static void calculateShadowViewMutationsFlattener(
                 parentTagForUpdate));
       }
 
-      auto adjustedOldCullingContext =
-          cullingContext.adjustCullingContextIfNeeded(oldTreeNodePair);
-      auto adjustedNewCullingContext =
-          cullingContext.adjustCullingContextIfNeeded(newTreeNodePair);
+      auto adjustedOldCullingContext = reparentMode == ReparentMode::Flatten
+          ? cullingContext.adjustCullingContextIfNeeded(oldTreeNodePair)
+          : cullingContextForUnvisitedOtherNodes.adjustCullingContextIfNeeded(
+                oldTreeNodePair);
+      auto adjustedNewCullingContext = reparentMode == ReparentMode::Flatten
+          ? cullingContextForUnvisitedOtherNodes.adjustCullingContextIfNeeded(
+                newTreeNodePair)
+          : cullingContext.adjustCullingContextIfNeeded(newTreeNodePair);
 
       // Update children if appropriate.
       if (!oldTreeNodePair.flattened && !newTreeNodePair.flattened) {
@@ -670,6 +678,7 @@ static void calculateShadowViewMutationsFlattener(
                    : parentTag),
               subVisitedNewMap,
               subVisitedOldMap,
+              cullingContext,
               cullingContext.adjustCullingContextIfNeeded(treeChildPair));
         } else {
           // Get flattened nodes from either new or old tree
@@ -720,6 +729,7 @@ static void calculateShadowViewMutationsFlattener(
                 fixedParentTagForUpdate,
                 subVisitedNewMap,
                 subVisitedOldMap,
+                adjustedNewCullingContext,
                 adjustedNewCullingContext);
           } else {
             // Flatten parent, unflatten child
@@ -740,6 +750,8 @@ static void calculateShadowViewMutationsFlattener(
                 /* parentTagForUpdate */ fixedParentTagForUpdate,
                 /* parentSubVisitedOtherNewNodes */ subVisitedNewMap,
                 /* parentSubVisitedOtherOldNodes */ subVisitedOldMap,
+                /* cullingContextForUnvisitedOtherNodes */
+                adjustedOldCullingContext,
                 /* cullingContext */ adjustedOldCullingContext);
 
             // If old nodes were not visited, we know that we can delete them