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lines changed Original file line number Diff line number Diff line change @@ -62,13 +62,13 @@ be concatenated using the `-` delimiter and placed in the given SAM record tag (
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default). Similarly, the sample barcode bases from the given read will be placed in the `BC`
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tag.
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- Metadata about the samples should be given as a headered metadata TSV file with at least the
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+ Metadata about the samples should be given as a headered metadata TSV file with at least the
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following two columns present:
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- 1. `sample_id` - the id of the sample or library.
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+ 1. `sample_id` - the id of the sample or library.
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2. `barcode` - the expected barcode sequence associated with the `sample_id`.
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- For reads containing multiple barcodes (such as dual-indexed reads), all barcodes should be
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+ For reads containing multiple barcodes (such as dual-indexed reads), all barcodes should be
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concatenated together in the order they are read and stored in the `barcode` field.
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The read structures will be used to extract the observed sample barcode, template bases, and
@@ -154,7 +154,7 @@ Options:
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[default: 2]
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-t, --threads <THREADS >
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- The number of threads to use. Cannot be less than 3
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+ The number of threads to use. Cannot be less than 5
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[default: 8]
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