Bio_informatics_science_technology_image_beyond_1/2026-02-03_keep-climbing.qmd at main · iamhealthy/Bio_informatics_science_technology_image_beyond_1 · GitHub

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---
title: Keep climbing
subtitle: Alex Honnold徒手无保护攀爬台北101
date: 2026-02-03
toc-depth: 4
toc-expand: true
lang: en
---

## 1. Alex Honnold徒手无保护攀爬台北101的一个瞬间

Alex Honnold在2026-01-24日徒手无保护攀爬了台北101大楼（ropeless climb of Taipei 101）。下面是一个攀爬的瞬间。

<center>
![图源：<https://www.netflix.com/tudum/articles/how-to-watch-alex-honnold-skyscraper-climb-live-netflix>](images/56_Alex_Honold.jpg){width="100%"}
</center>

## 2. 通过QuPath进行color deconvolution（颜色解卷积）

### 2.1 设定图片格式为Brightfield H&E时

把图片通过QuPath打开，并color deconvolution后，发现Eosin通道的图片貌似有些[艺术感]{.mark}。

<img src="images/56_Alex_Honold_eosin.png" style="width:100%; border:1px solid gray">

这是对应的groovy script [@KW_eosin2026]：

```
// QuPath v0.6.0

import qupath.lib.images.servers.TransformedServerBuilder
setImageType('BRIGHTFIELD_H_E')
setColorDeconvolutionStains('{"Name" : "H&E default", "Stain 1" : "Hematoxylin", "Values 1" : "0.6511078257574492 0.7011930431234068 0.29049426072255424", "Stain 2" : "Eosin", "Values 2" : "0.21589893562087106 0.8011960501132093 0.5580972485873467", "Background" : " 255 255 255"}');

// 1 for Hematoxylin, 2 for Eosin, 3 for Residual
var stainNumber = 2

var server = getCurrentServer()
var imageData = getCurrentImageData()

var stains = imageData.getColorDeconvolutionStains()
// Scale factors: 0.0, 1.0, 0.0 for Eosin
// Scale factors: 1.0, 0.0, 0.0 if for Hematoxylin
// the exported image might look slightly darker than in the QuPath viewer,
// since the viewer applies brightness/contrast adjustments.
// You can tweak the scale factor (e.g. 0.85 or other values instead of 1.0) if you want to lighten it.
var extractedServer = new TransformedServerBuilder(server)
        .stainNormalize(stains, stains, 0.0, 1.0, 0.0)
        .build()

def name = getCurrentImageNameWithoutExtension()
def fileName = "${name}_eosin.png"

// Set the folder to save images
def folder = 'path\\to\\your\\folder'
def outputFile = new File(buildFilePath(folder, fileName))

// Write the full image (only possible if it isn't too large!)
writeImage(extractedServer, "${outputFile}")

println "${outputFile} saved!"
```

### 2.2 设定图片格式为Brightfield H-DAB时

DAB通道的图片貌似亦有些[艺术感]{.mark}。

<image src="images/56_Alex_Honold_dab.png" style="width:100%; border:1px solid gray">

对应的groovy script和`2.1`部分类似，只需将：

```
setImageType('BRIGHTFIELD_H_E')
setColorDeconvolutionStains('{"Name" : "H&E default", "Stain 1" : "Hematoxylin", "Values 1" : "0.6511078257574492 0.7011930431234068 0.29049426072255424", "Stain 2" : "Eosin", "Values 2" : "0.21589893562087106 0.8011960501132093 0.5580972485873467", "Background" : " 255 255 255"}');

some codes

def fileName = "${name}_eosin.png"
```

更改为：

```
setImageType('BRIGHTFIELD_H_DAB')
setColorDeconvolutionStains('{"Name" : "H-DAB default", "Stain 1" : "Hematoxylin", "Values 1" : "0.6511078257574492 0.7011930431234068 0.29049426072255424", "Stain 2" : "DAB", "Values 2" : "0.26916687204956063 0.5682411743268502 0.777593185920953", "Background" : " 255 255 255"}');

some codes

def fileName = "${name}_dab.png"
```

[给我买杯茶🍵](给我买杯茶.qmd)

## References