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2026-03-04_切片染色方法.qmd
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62 lines (41 loc) · 1.77 KB
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---
title: 切片染色方法
subtitle: 几个组织切片多重免疫染色的方法
date: 2026-03-06
toc-depth: 4
toc-expand: true
lang: en
---
直接展示其原理图，不做过多描述。
## 1. IMC: imaging mass cytometry
> coupled immunohistochemical and immunocytochemical methods with high-resolution laser ablation to CyTOF mass cytometry [@Giesen2014].
<center>
{width="100%"}
</center>
## 2. CODEX: CO-Detection by indEXing
> Multiplexed DNA-tagged antibody staining [@Goltsev2018].
<center>
{width="100%"}
</center>
## 3. t-CyCIF: tissue-based cyclic immunofluorescence
> conventional low-plex fluorescence images are repeatedly collected from the same sample and then assembled into a high-dimensional representation [@Lin2018].
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{width="100%"}
</center>
## 4. 4i: iterative indirect immunofluorescence imaging
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{width="100%"}
![From [@Gut2018]](images/2026-03-04_science_2018_4i_legend.jpg){width="100%"}
</center>
## 5. CODA: quantitative 3D reconstruction of large tissues at cellular resolution
> CODA maps whole organs and organisms at single-cell resolution [@Kiemen2022], [@denis-wirtz2026].
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{width="100%"}
</center>
## 6. iCLAP: integrable Co-detection of Low-Abundant Proteins
> enables sensitive and highly multiplexed protein detection within the same FFPE tissue section [@Wu2026].
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{width="100%"}
</center>
[给我买杯茶🍵](给我买杯茶.qmd)
## References