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README.md

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[![DOI](https://zenodo.org/badge/DOI/10.5281/zenodo.3367200.svg)](https://doi.org/10.5281/zenodo.3367200)
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# `immunarch` - An R Package for Painless Analysis of Large-Scale Immune Repertoire Data
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# `immunarch` - An R Package for Painless Bioinformatics Analysis of T-cell and B-cell Immune Repertoire Data
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- [Introduction](#introduction)
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- [Installation](#installation)
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## Installation
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In order to install `immunarch` execute the following R command:
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In order to install `immunarch` execute the following R commands:
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```r
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install.packages("immunarch")
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install.packages("devtools") # skip this if you already installed devtools
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devtools::install_url("https://github.com/immunomind/immunarch/raw/master/immunarch.tar.gz")
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```
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That's it, you can start using `immunarch` now! See the [Quick Start](#quick-start) section below to dive into immune repertoire data analysis. If you run in any trouble with installation, take a look at the [Installation Troubleshooting](#installation-troubleshooting) section below.
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That's it, you can start using `immunarch` now! See the [Quick Start](#quick-start) section below to dive into immune repertoire data analysis. If you run in any trouble with installation, take a look at the [Installation Troubleshooting](https://immunarch.com/articles/v1_introduction.html#installation-troubleshooting) section.
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Note that there are quite a lot of dependencies to install with the package because it installs all the widely-used packages for data analysis and visualisation. You got both the AIRR data analysis framework and Data Science package eco-system with only one command!
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You can find the list of releases of `immunarch` here: https://github.com/immunomind/immunarch/releases
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<!--
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### Pre-release version installation
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Since releasing on CRAN is limited to one release per one-two months, you can install the latest pre-release version with bleeding edge features and optimisations directly from a code repository. In order to install the latest pre-release version, you need to execute only two commands:
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install.packages("devtools") # skip this if you already installed devtools
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devtools::install_url("https://github.com/immunomind/immunarch/raw/master/immunarch.tar.gz")
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```
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-->
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You can find the list of releases of `immunarch` here: https://github.com/immunomind/immunarch/releases
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## Features
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+ Most methods are incorporated in a couple of main functions with clear naming---no more remembering tens and tens of functions with obscure names. For details see [link](https://immunarch.com/articles/v3_basic_analysis.html);
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+ Repertoire overlap analysis *(common indices including overlap coefficient, Jaccard index and Morisita's overlap index)*. Tutorial is available [here](https://immunarch.com/articles/v4_overlap.html);
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+ Repertoire overlap analysis *(common indices including overlap coefficient, Jaccard index and Morisita's overlap index)*. Tutorial is available [here](https://immunarch.com/articles/web_only/v4_overlap.html);
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+ Gene usage estimation *(correlation, Jensen-Shannon Divergence, clustering)*. Tutorial is available [here](https://immunarch.com/articles/v5_gene_usage.html);
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+ Gene usage estimation *(correlation, Jensen-Shannon Divergence, clustering)*. Tutorial is available [here](https://immunarch.com/articles/web_only/v5_gene_usage.html);
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+ Diversity evaluation *(ecological diversity index, Gini index, inverse Simpson index, rarefaction analysis)*. Tutorial is available [here](https://immunarch.com/articles/v6_diversity.html);
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+ Diversity evaluation *(ecological diversity index, Gini index, inverse Simpson index, rarefaction analysis)*. Tutorial is available [here](https://immunarch.com/articles/web_only/v6_diversity.html);
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+ Tracking of clonotypes across time points, widely used in vaccination and cancer immunology domains. Tutorial is available [here](https://immunarch.com/articles/v8_tracking.html);
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+ Tracking of clonotypes across time points, widely used in vaccination and cancer immunology domains. Tutorial is available [here](https://immunarch.com/articles/web_only/v8_tracking.html);
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+ Kmer distribution measures and statistics. Tutorial is available [here](https://immunarch.com/articles/v9_kmers.html);
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+ Kmer distribution measures and statistics. Tutorial is available [here](https://immunarch.com/articles/web_only/v9_kmers.html);
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+ Coming in the next releases: CDR3 amino acid physical and chemical properties assessment, mutation networks.
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+ Built-in tool `FixVis` makes your plots publication-ready: easily change font sizes, text angles, titles, legends and many more with clear-cut GUI;
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+ Tutorial is available [here](https://immunarch.com/articles/v7_fixvis.html).
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+ Tutorial is available [here](https://immunarch.com/articles/web_only/v7_fixvis.html).
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## Quick start
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The package is freely distributed under the Apache v2 license. You can read more about it [here](https://tldrlegal.com/license/apache-license-2.0-(apache-2.0)).
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Additionally, we provide an annual subscription that includes next services:
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If you are interested in our commercial-grade platform for biomarker discovery and AIRR analysis, contact us via [email protected]
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<!--
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- Package modifications and feature implementations are issued promptly;
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- Use *immunarch* team expertise in your projects;
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- Priority email and call support;
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- If you need license other than the current, contact us.
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Contact us at [email protected] for more information.
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-->

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