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AlphaFold Experimental Overlay

Open the experimental 4HHB tetramer and an AlphaFold hemoglobin alpha chain in ChimeraX, preserve a full-assembly view, then move into a chain-level overlay near the heme site and export the comparison.

  • App: chimerax
  • Difficulty: deep-dive
  • Estimated time: 8 minutes
  • Voice mode: push_to_talk
  • Last verified: 2026-04-04

Sample Data

  • Human deoxy hemoglobin tetramer: ./examples/data/local/4hhb.pdb
  • AlphaFold hemoglobin alpha chain: ./examples/data/local/af-p69905.pdb

What Success Looks Like

  • The experimental tetramer and AlphaFold chain are both open in ChimeraX.
  • The AlphaFold chain is aligned to experimental chain A.
  • Named views exist for the assembly overview, the overlay focus, and the exploded comparison.
  • A polished overlay export is written successfully.