diff --git a/setup.py b/setup.py
index 0afb8106..413cb734 100644
--- a/setup.py
+++ b/setup.py
@@ -46,13 +46,6 @@ def run_tests(self):
     "PyYAML",
     "wrapt",
     "yarl",
-    # Support for urllib3 >=2 needs Python >=3.10
-    # so we need to block urllib3 >=2 for Python <3.10 for now.
-    # Note that vcrpy would work fine without any urllib3 around,
-    # so this block and the dependency can be dropped at some point
-    # in the future. For more Details:
-    # https://github.com/kevin1024/vcrpy/pull/699#issuecomment-1551439663
-    "urllib3 <2; python_version <'3.10'",
 ]
 
 tests_require = [
diff --git a/tox.ini b/tox.ini
index 62035131..848c2351 100644
--- a/tox.ini
+++ b/tox.ini
@@ -4,7 +4,7 @@ envlist =
   cov-clean,
   lint,
   {py38,py39,py310,py311,py312}-{requests-urllib3-1,httplib2,urllib3-1,tornado4,boto3,aiohttp,httpx},
-  {py310,py311,py312}-{requests-urllib3-2,urllib3-2},
+  {py38,py39,py310,py311,py312}-{requests-urllib3-2,urllib3-2},
   {pypy3}-{requests-urllib3-1,httplib2,urllib3-1,tornado4,boto3},
   #{py310}-httpx019,
   cov-report