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Hi there,
When I am using octopus v0.7.4 to call germline SV from 1 bam file without forest model and seq_error_model, it throws an error:
[2024-04-11 17:06:33] <INFO> ------------------------------------------------------------------------
[2024-04-11 17:06:33] <INFO> octopus v0.7.4
[2024-04-11 17:06:33] <INFO> Copyright (c) 2015-2021 University of Oxford
[2024-04-11 17:06:33] <INFO> ------------------------------------------------------------------------
[2024-04-11 17:06:36] <EROR> A user error has occurred:
[2024-04-11 17:06:36] <EROR>
[2024-04-11 17:06:36] <EROR> The region Chr1:0-76456565 is after Chr19:0-51544995 in reference
[2024-04-11 17:06:36] <EROR> index.
[2024-04-11 17:06:36] <EROR>
[2024-04-11 17:06:36] <EROR> To help resolve this error ensure the region range format is <lhs> to
[2024-04-11 17:06:36] <EROR> <rhs> where lhs occurs before rhs in the reference index.
[2024-04-11 17:06:36] <INFO> ------------------------------------------------------------------------
octopus --version says
octopus version 0.7.4
Target: x86_64 Linux 5.11.0-1028-azure
SIMD extension: AVX512
Compiler: GNU 10.3.0
Boost: 1_74what I knew is I am using a non-human genome, and the seq names aren't sorted, my command is as follows:
octopus -R /mnt/caller/ref/genome.fasta -I /mnt/caller/rgbam/230.bam -T Chr1 to Chr19 --threads 1 -o 230.vcf.gz
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