Allowing multiple pools per flowcell or Pools of pools

[charlesgregory]

My lab performs a large amount of targeted high-throughput sequencing for several other labs. Currently data management of our requests is less than ideal and your LIMS is wonderful and would be a great fit for our lab. Our library prep usually follows a similar workflow as the model you have used for Parkour. I was wondering if it would be possible to allow multiple pools per lane on a flowcell or allow pools of pools. Our current workflow involves pooling several samples (~12 at a time) together, performing a targeted capture, then pooling those pools after capture together (~ 6 pools ~72 samples total) for loading a lane of the flowcell. Allowing multiple pools per lane would allow us to just represent the second pooling as being all the pools in the lane. Allowing pools of pools could also work but I imagine it would be more difficult.

[dpryan79]

Am I correct in assuming that the capture step with the small pools is performed after library prepartion is complete (i.e., barcodes have been added)? I think the easiest implementation of this would be to allow making pools of pools (a "water park"?) and allowing loading of either those or regular pools on flow cells. A pool of pools would facilitate checking barcode compatibility (assuming you're not doing inline sample barcodes for this).

I'll chat with @anatskiy about this when he gets in. He needs to finish his thesis before implementing new features, but if our sequencing facility could find this useful then perhaps I can attempt implementing it.

[charlesgregory]

Yes we perform library prep with dual indexed adapters and then capture is performed.