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| 1 | +#------------------------------------------------------------ |
| 2 | +# Values for this lesson. |
| 3 | +#------------------------------------------------------------ |
| 4 | + |
| 5 | +# Which carpentry is this (swc, dc, lc, or cp)? < REPLACED with UNIMELB institutes > |
| 6 | +# uom: University of Melbourne |
| 7 | +# mb: Melbourne Bioinformatics |
| 8 | +# mig: Melbourne Integrative Genomics |
| 9 | +# wehi: Walter and Eliza Hall Institute |
| 10 | +carpentry: 'mb' |
| 11 | + |
| 12 | +# Alt-text description of the lesson. |
| 13 | +carpentry_description: 'Lesson Description' |
| 14 | + |
| 15 | +# Overall title for pages. |
| 16 | +title: 'Introduction to RNA-seq analysis' |
| 17 | + |
| 18 | +# Date the lesson was created (YYYY-MM-DD, this is empty by default) |
| 19 | +created: '2025-08-13' |
| 20 | + |
| 21 | +# Comma-separated list of keywords for the lesson |
| 22 | +keywords: RNA-seq, Biological replicates, Sequencing depth, Biological |
| 23 | + variability, Technical replicates, RNA quality, Enrichment method, |
| 24 | + Experimental design |
| 25 | + |
| 26 | +# Life cycle stage of the lesson |
| 27 | +# possible values: pre-alpha, alpha, beta, stable |
| 28 | +life_cycle: 'alpha' |
| 29 | + |
| 30 | +# License of the lesson |
| 31 | +license: 'CC-BY 4.0' |
| 32 | + |
| 33 | +# Link to the source repository for this lesson |
| 34 | +source: 'https://github.com/melbournebioinformatics/intro-to-rna-seq' |
| 35 | + |
| 36 | +# Default branch of your lesson |
| 37 | +branch: 'main' |
| 38 | + |
| 39 | +# Who to contact if there are any issues |
| 40 | +contact: 'jiayi.si.2[ at ]unimelb.edu.au' |
| 41 | + |
| 42 | +# Navigation ------------------------------------------------ |
| 43 | +# |
| 44 | +# Use the following menu items to specify the order of |
| 45 | +# individual pages in each dropdown section. Leave blank to |
| 46 | +# include all pages in the folder. |
| 47 | +# |
| 48 | +# Example ------------- |
| 49 | +# |
| 50 | +# episodes: |
| 51 | +# - introduction.md |
| 52 | +# - first-steps.md |
| 53 | +# |
| 54 | +# learners: |
| 55 | +# - setup.md |
| 56 | +# |
| 57 | +# instructors: |
| 58 | +# - instructor-notes.md |
| 59 | +# |
| 60 | +# profiles: |
| 61 | +# - one-learner.md |
| 62 | +# - another-learner.md |
| 63 | + |
| 64 | +# Order of episodes in your lesson |
| 65 | +episodes: |
| 66 | +- introduction.Rmd |
| 67 | +- what-is-RNA-seq.Rmd |
| 68 | +- why-is-a-good-experimental-design-vital.Rmd |
| 69 | +- terminology.Rmd |
| 70 | +- the-importance-of-replicates-to-estimate-variance.Rmd |
| 71 | +- how-many-replicates-and-how-many-reads-do-I-need.Rmd |
| 72 | +- sequencing-options-to-consider.Rmd |
| 73 | +- summary.Rmd |
| 74 | + |
| 75 | +# Information for Learners |
| 76 | +learners: |
| 77 | + |
| 78 | +# Information for Instructors |
| 79 | +instructors: |
| 80 | + |
| 81 | +# Learner Profiles |
| 82 | +profiles: |
| 83 | + |
| 84 | +# Customisation --------------------------------------------- |
| 85 | +# |
| 86 | +# This space below is where custom yaml items (e.g. pinning |
| 87 | +# sandpaper and varnish versions) should live |
| 88 | + |
| 89 | +# This is the UoM varnish, which is a custom version of the Carpentries varnish |
| 90 | +varnish: 'melbournebioinformatics/uom-varnish@main' |
| 91 | +url: 'melbournebioinformatics.github.io/uom-varnish' |
| 92 | + |
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